Using phylogenetic probes for quantification of stable isotope labeling and microbial community analysis

ABSTRACT

Herein is described methods for a high-sensitivity means to measure the incorporation of stable isotope labeled substrates into RNA following stable isotope probing experiments (SIP). RNA is hybridized to a set of probes such as phylogenetic microarrays and isotope incorporation is quantified such as by secondary ion mass spectrometer imaging (NanoSIMS).

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims priority to U.S. Provisional Patent Application No. 61/302,535, filed on Feb. 8, 2010 and to U.S. Provisional Patent Application No. 61/302,827 filed on Feb. 9, 2010, both of which are hereby incorporated by reference. This application is related to concurrently filed U.S. patent application Ser. No. 13/023,468, filed on Feb. 8, 2011, entitled “Devices, Methods and Systems for Targeted Detection,” which claims priority to these same U.S. Provisional Patent applications and is hereby incorporated by reference in its entirety.

STATEMENT OF GOVERNMENTAL SUPPORT

This invention was made in part by the US DOE Office of Biological and Environmental Research Genomic Sciences research program and the LLNL Laboratory Directed Research and Development (LDRD) program with government support under Contract No. DE-AC02-05CH11231 and under Contract DE-AC52-07NA27344 awarded by the U.S. Department of Energy. The government has certain rights in the invention.

REFERENCE TO SEQUENCE LISTING AND TABLES

This application hereby incorporates the attached sequence listing in computer readable form and the attached Table 1 showing the sequences SEQ ID NOS:1-2805.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates to methods of using probes and microarrays to measure multiple different stable isotopes in nucleic acids and identification and analysis of microbial communities.

2. Related Art

Identification of microorganisms responsible for specific metabolic processes remains a major challenge in environmental microbiology, one that requires the integration of multiple techniques.

Nucleic acid stable isotope probing (SIP) techniques (5, 6) are currently the most widely used means to directly connect specific substrate utilization to microbial identity, a grand challenge in the field of microbial ecology (7). For traditional SIP, natural microbial communities are incubated in the presence of a substrate enriched in a rare stable isotope (either ¹³C or ¹⁵N). The organisms, including their nucleic acids, incorporate the substrate and become isotopically enriched over time. Ultracentrifugation is used to separate isotopically enriched nucleic acids from lighter, unenriched nucleic acids for molecular analysis. In the past decade, these approaches have generated many advances in the understanding of microbial bioremediation, plant-microbe interactions and food web dynamics (8), yet they remain hindered by logistical drawbacks (9). These issues are intensified when working with density-gradient centrifugation of RNA, where the focus is on active organisms that are not necessarily replicating. Most notably, traditional DNA- and RNA-SIP isotope exposure risks fertilization effects by requiring high substrate concentrations in order to meet the sensitivity threshold of density gradient separation (in many systems>20% ¹³C DNA) (10) and is extremely difficult to perform with ¹⁵N labeled substrates (>40% ¹⁵N DNA required) (11). Other disadvantages include long exposure times (risking community cross-feeding), low-throughput (1-2 weeks lab processing time per sample batch), and incomplete quantification. Though related culture-independent approaches also have ideal qualities such as high sensitivity or in situ resolution (e.g. ¹³C-PLFA (12); EL FISH (13), FISH MAR (14), isotope arrays (15)), none combines high throughput, sensitivity, taxonomic resolution, and quantitative estimates of multiple stable isotope (¹⁵N and ¹³C) incorporation.

BRIEF SUMMARY OF THE INVENTION

The present invention provides a method for quantification of stable isotope labeling using phylogenetic probes.

In another aspect, the present invention comprises community analysis using such phylogenetic probes.

The methods described have the ability to track the update of carbon, nitrogen and oxygen in ribonucleic acids and providing insight into how microorganisms metabolize these elements. The methods as described can track the uptake of carbon and nitrogen simultaneously and also be applied to oxygen. There is no other known method that can track the uptake of carbon and nitrogen simultaneously.

A method for determination of stable isotope incorporation in a organism or a community of organisms comprising the steps of: (a) supplying an organism or said community of organisms with a stable isotope labeled substrate for a defined period of time; (b) extracting RNA from the organisms; (c) fragmenting said RNA; (d) labeling a fraction the fragmented RNA with a detectable label; (e) hybridizing the labeled RNA to a set of oligonucleotide probes; (f) detecting hybridization signal strength of labeled RNA hybridized to any of the oligonucleotide probes and identifying and selecting the hybridized oligonucleotide probes as a responsive set of probes; (g) hybridizing a fraction of unlabeled RNA to a second set of oligonucleotide probes comprising the responsive set of probes; (h) detecting the unlabeled RNA hybridized to the responsive set of probes to determine the stable isotope incorporation into the organism using spectrometry or spectroscopy.

In one embodiment, the organism is a bacterium, archaea, fungi, plant, arthropod, or nematode, or other eukaryote. In a specific embodiment, the organism is a bacterium.

In one embodiment, the stable-isotope labeled substrate is ³H, ¹³C ¹⁵N, and/or ¹⁸O.

Extraction of RNA can be carried out by physical and/or chemical cell lysis and affinity column purification. Fragmentation is generally carried out by using either enzymes or chemicals or heat or a combination of these. A fraction or aliquot of the RNA is then labeled with a fluorescent molecule or a non-fluorescent molecule. Fragmentation and labeling can occur in some embodiments concurrently.

In one embodiment, the set of oligonucleotide probes comprising an array of oligonucleotide probes attached to a substrate such as a microarray or chip. The labeled fragmented RNA can then be added to a hybridization solution and the hybridization solution contacted to the array of oligonucleotide probes to allow the labeled RNA to hybridize to the probes.

In one embodiment, the set of oligonucleotide probes comprising 16S rRNA phylogenetic oligonucleotide probes. The set of 16S rRNA phylogenetic probes further comprising probes from the 16S rRNA gene, 23S rRNA gene, 5S rRNA gene, 5.8S rRNA gene, 12S rRNA gene, 18S rRNA gene, 28S rRNA gene, gyrB gene, rpoB gene, fusA gene, recA gene, cox1 gene, nif13 gene, RNA molecules derived therefrom, or a combination thereof.

The array with the hybridized labeled RNA is imaged with a fluorescence scanner and fluorescence intensity measured for each probe feature and the detection of hybridization signal strength provides a determination of the genes present in a organism or genes and/or organisms present in the community of organisms. The detection of hybridization signal strength also provides a means for normalization of the isotope signals detected.

In one embodiment, the probes that hybridized to the labeled RNA are synthesized onto a second array of oligonucleotide probes comprising down-selected probes or responsive probes. The unlabeled RNA is hybridized to the second array hybridized unlabeled RNA are imaged with a with a secondary ion mass spectrometer and isotope ratios are measured for each probe feature.

The presently described methods provide high throughput, sensitivity, taxonomic resolution, and quantitative estimates of multiple stable isotope (¹⁵N and ¹³C) incorporation. In one embodiment, microbial identity and function are connected by isolating rRNA from individual taxa through hybridization to phylogenetic probes. In one embodiment, the probes are displayed on a substrate surface, such as a custom glass microarray. After hybridization, these probe features are then analyzed for isotope enrichment. In some embodiments, the probes are analyzed using analysis techniques including but not limited to, spectrometry, spectroscopy, and quantitative secondary ion mass spectrometry imaging.

Direct NanoSIMS analysis is made possible by implementing a new surface chemistry for synthesis of DNA on conductive material. With this approach, thousands of unique phylogenetic probes assaying hundreds of taxa can be quickly analyzed from a single sample.

The present methods may be used in applications such as the evaluation of how certain organisms metabolize cellulose and what enzymes they use to do this; evaluation of what organisms have the ability to degrade pollutants in an environmental sample such as oil using water samples from the recent Gulf oil spill; or a study of carbon sequestration.

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A and 1B. Hybridization of extracted RNA from a single bacterial species (Pseudomonas stutzeri) grown on ¹³C-glucose as the sole carbon source. Each spot (and data point) represents a distinct probe specific for Pseudomonas. FIG. 1: (A) fluorescence image and (B) NanoSIMS isotopic enrichment image montage of a microarray hybridized with RNA from a single bacterial strain (Pseudomonas stutzeri) grown on ¹³C glucose.

FIG. 2A is a graph that shows that fluorescence and ¹³C enrichment are positively correlated demonstrating successful detection of labeled RNA in FIG. 1. FIGS. 2B and 2C show Chip-SIP analysis of two strains with differential isotopic enrichment demonstrating clear separation of the two taxa; Vibrio cholerae (gray squares), Bacillus cereus (gray triangles), background (black diamond). HCE=hybridization-corrected enrichment. Each point represents an individual probe spot's fluorescence intensity value (a measure of fluorescence) plotted against its isotopic enrichment measured by NanoSIMS. Error bars are SEMs based on total ion counts.

FIG. 3 shows two array images of RNA enriched with 0.5% ¹³C successfully detected by phylogenetic probes.

FIG. 4A shows two array images of Pseudomonas stutzeri grown on 25% ¹⁵N ammonium, Bacillus cereus grown on natural abundance ammonium; RNA extracted, mixed in equal concentrations, hybridized on array with phylogenetic probes. FIG. 4B shows a graph of the fluorescence intensity and ¹³C enrichment for Pseudomonas stutzeri grown on 100% ¹³C glucose, Vibrio cholera grown on 20% ¹³C glucose (images are not shown). A graph on the bottom panel shows one-way ANOVA analysis which demonstrates that the method is semi-quantitative because one taxa is more enriched than the other.

FIG. 5. San Francisco Bay water collected at Berkeley pier, incubated with 200 uM ¹⁵N ammonium for 24 hours. FIG. 5A shows array images of a marine microbes array designed using ARB (Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar et al. 2004. Nucl. Acids Res. 32:1363-1371); each row represents a different taxon. FIG. 5B is a graph showing the ¹⁵N enrichment in various taxa over time.

FIG. 6 is a graph showing the ¹³C enrichment in various taxa after incubation. San Francisco Bay water collected at Berkeley pier, incubated with 50 μM ¹³C amino acids (98% ¹³C), 30 μM fatty acids (98% ¹³C), and 10 mg L-1 starch (10% ¹³C) for 12 hours. Additional probes for larger phylogenetic groups (bacteria, Rhodobacteriacea, Planctomycetes, Marine Group A) designed using ARB.

FIG. 7 shows substrate incorporation detected by chip-SIP for a SF Bay estuarine microbial community incubated with 200 μM¹⁵N ammonium and 50 μM¹³C glucose for 24 hrs; A) ¹⁵N ammonium and B) ¹³C glucose incorporation for 3 taxa within the bacterial family Rhodobacteriaceae; each point is derived from a single probe spot's isotopic enrichment value plotted against fluorescence (a measurement of hybridization); C) relationship between ammonium and glucose incorporation for 16 taxa from two bacterial families; HCE=hybridization-corrected enrichment; arrows indicate taxa plotted in A and B.

FIG. 8A shows a network map of Chip-SIP analysis of the uptake patterns of three organic substrates by different bacterial taxa in an estuary, identifying substrate specialists and generalists; the thicknesses of the lines are proportional to the substrate incorporation rates based on HCE calculations (Flavo=Flavobacteriaceae, Roseo=Roseobacter, MarGrpA=Marine Group A). FIG. 8B shows a heat map relationship between substrate incorporation (green=detected, black=not detected) and 16S rRNA phylogeny for a subset of the Gammaproteobacteria, indicating taxa where physiological traits match phylogeny (Alteromonadales and Vibrionaceae) and where they do not (Oleispira).

FIG. 9 shows the relationship between array fluorescence (a metric of RNA hybridization) and ¹³C/¹²C enrichment (analyzed by NanoSIMS) of RNA from Pseudomonas stutzeri cultures grown separately on two levels of ¹³C-glucose as a sole carbon source and hybridized to an indium tin oxide (ITO) microarray. Each point represents data from a single probe location on the array. The fluorescence:enrichment relationship (i.e. hybridization corrected enrichment, “HCE”) is both highly significant (see regression statistics) and different between RNA from cultures with 100% ¹³C (gray) versus 5% ¹³C enriched cultures (dark gray).

FIG. 10: Relative incorporation of ¹⁵N-ammonium and ¹³C-glucose detected by chipSIP for a natural estuarine microbial community from the San Francisco Bay. Units are the slope of permil isotope enrichment over fluorescence (HCE=hybridization corrected enrichment). Each point is the average of probe spots representing the identified phyla. Error bars represent the standard error of the slope calculation.

FIG. 11: Relative incorporation of amino acids and nucleic acids detected by chip-SIP for a natural estuarine microbial community from the San Francisco Bay. Units are the slope of permil enrichment over fluorescence (HCE=hybridization corrected enrichment).

FIG. 12: Relative incorporation of amino acids and fatty acids detected by chip-SIP for a natural estuarine microbial community. Units are the slope of permil enrichment over fluorescence (HCE=hybridization corrected enrichment).

FIG. 13: Relative incorporation of fatty acids and nucleic acids detected by chip-SIP for a natural estuarine microbial community. Units are the slope of permil enrichment over fluorescence (HCE=hybridization corrected enrichment).

DETAILED DESCRIPTION

Initial experiments utilized a single bacterial strain (Pseudomonas stutzeri) grown on ¹³C glucose as the sole carbon source to determine the feasibility of successful hybridization of extracted RNA on the microarray surface, and detection of ¹³C from the hybridized RNA. FIG. 1 shows images of arrays of hybridization of extracted RNA from a single bacterial species (Pseudomonas stutzeri) grown on ¹³C-glucose as the sole carbon source. Each spot (and data point) represents a distinct probe specific for Pseudomonas. The results show fluorescence (a measure of how much RNA is hybridized) and ¹³C enrichment are positively correlated, demonstrating successful detection of labeled RNA with the Phylochip probe array.

Referring now to FIG. 3, the limits of prior detection methods of isotopic enrichment are not seen using the present probes and using such analysis methods as nanoSIMS (nanoscale secondary ion mass spectrometry). Traditional SIP (Stable Isotope Probing) requires approximately 10 atom % isotopic enrichment for detection (Radajewski S, Ineson P, Parekh N R & Murrell J C 2000. Nature 403: 646-649). We have successfully detected hybridized RNA from Pseudomonas stutzeri grown in 0.5 atom % ¹³C glucose. RNA enriched with 0.5% ¹³C successfully detected.

FIG. 4 shows results of experiments with artificial mixed communities. Before testing the method in the environment, we mixed RNA from different bacterial strains grown on different levels of ¹³C or ¹⁵N to determine cross-hybridization potential. An experiment was carried out with a simple two-member community: Pseudomonas stutzeri grown on 25% ¹⁵N ammonium, Bacillus cereus grown on natural abundance ammonium; RNA extracted, mixed in equal concentrations, hybridized on array featuring Phylochip probes. Experiment 2: Pseudomonas stutzeri grown on 100% ¹³C glucose, Vibrio cholera grown on 20% ¹³C glucose (images are not shown). The results of these two experiments shows that unlabeled taxa do not show isotopic signal in NanoSIMS, and that the present method can potentially be semi-quantitative (e.g. one taxon is more enriched than another).

FIG. 5 shows the first trial of method with natural microbial communities: To apply the method to the environment, we designed a 16S rRNA and 18S rRNA microarray for common marine microbial taxa (bacteria, archaea, and protists) targeting specific phylotypes (approximately at the genus level). Estuarine samples were incubated in the presence of ¹⁵N ammonium and sampled over time. Application of the present method using the phylogenetic probes to samples collected in San Francisco Bay water collected at Berkeley pier, incubated with 200 uM ¹⁵N ammonium for 24 hours. Marine microbes array designed using ARB (Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar et al. 2004. Nucl. Acids Res. 32:1363-1371); each row represents a different taxon. FIGS. 5A and 5B show that different taxa incorporate ammonia at different rates. The microarray probes are found in the accompanying Sequence Listing and identified as SEQ ID NOS:1-2805.

Little is known about organic carbon incorporation patterns in marine and estuarine environments, partly because the dominant organisms are uncultured and cannot be directly interrogated in the laboratory. We used the Chip-SIP method to test whether different taxa incorporate amino acids, fatty acids, and starch for their carbon growth requirements.

FIG. 6 shows the use of Chip-SIP method to identify organic matter utilization in estuarine microbial communities in San Francisco Bay water collected at Berkeley pier. The samples were incubated with 50 μM ¹³C amino acids (98% ¹³C), 30 μM fatty acids (98% ¹³C), and 10 mg L-1 starch (10% ¹³C) for 12 hours. Additional probes for larger phylogenetic groups (bacteria, Rhodobacteriacea, Planctomycetes, Marine Group A) were designed using ARB. As shown in FIG. 6, different microbial taxa incorporated different substrates in situ. All tested substrates were incorporated by some bacteria. One taxon (acido4) appeared to be a generalist, while all other taxa demonstrated some degree of specificity in the substrates that were incorporated into biomass.

Thus, in one embodiment, the present invention provides methods for quantification of stable isotope labeling to observe and measure resource partitioning in microbial communities using phylogenetic probes. In one embodiment, the phylogenetic probes can be designed. In another embodiment, phylogenetic probes previously designed and provided in the previous applications hereby incorporated by reference can be used.

In one embodiment, such a method involves labeling microbial nucleic acids with stable isotope-labeled substrates (e.g, ¹³C-amino acids, cellulose or ¹⁵NH₄). Current methods for stable-isotope probing require large quantities of label to be incorporated into nucleic acids prior to density gradient separation (e.g. refs. Radajewski S, Meson P, Parekh N R & Murrell J C 2000. Nature 403: 646-649; Manefield M., Whiteley, A. S., Griffiths, R. I. and Bailey, M. J. 2002. Appl. Environ. Microbiol. 68:5367-73), however the necessary quantities of labeled substrate often impose a significant disturbance on system energy and metabolite flux. The presently described approach is to capture ribosomal RNA using sequence specific probes targeting 16S rRNA (Brodie, E. L., T. Z. DeSantis, D. C. Joyner, S. M. Baek, J. T. Larsen, G. L. Andersen, T. C. Hazen, P. M. Richardson, D. J. Herman, T. K. Tokunaga, J. M. M. Wan, and M. K. Firestone. 2006. Appl. Environ. Microbiol. 72:6288-6298), and the captured RNA is then analyzed for isotope ratios. Microarrays represent the highest-throughput approach for RNA capture; combining this with analysis methods allows isotope ratios to be determined for potentially hundreds of species within complex communities.

In some embodiments, the methods provides for a method comprising steps as the following described process. An organism or multiple organisms, such as a community of organisms, are supplied with a stable-isotope (e.g., ³H, ¹³C, ¹⁵N, ¹⁸O) labeled substrate for a defined period of time. RNA is extracted from the organisms or community organisms using any number of established procedures as is known in the art.

The organism RNA is fragmented using known fragmentation methods including use of enzymes, chemicals or heat or a combination of these. A first fraction or an aliquot of fragmented RNA is labeled with a fluorescent molecule or a non-fluorescently labeled molecule such as biotin. This can occur concurrently with fragmentation in some embodiments.

The labeled fraction of fragmented RNA is added to a hybridization solution and hybridized to a microarray slide. Weakly bound RNA can be removed from the microarray surface by washing in solutions of varying stringency. The RNA that is hybridized to the probes are then imaged to detect hybridization signal strength and thereby quantify the labeled RNA to determine the community organism composition and also to correct and normalize the isotope signals in the RNA bound to each probe.

Currently the organism composition and normalization of isotope signal occurs on a different device than the fluorescent detection of hybridization signal strength and measurement of isotope ratio or isotope incorporation. In such a case, the fluorescent detection provides a subset of responsive probes that correlate to the presence of a specific gene and/or an organism in the sample or the community. After this detection, the organisms are identified and a down-selected probe analysis is carried out. New probes to identify an organism can be designed, or the same probes from the larger set of oligonucleotide probes can be used. For example, in some instances, sequence information generated from reverse-transcribed RNA (cDNA) from the same samples is used to select unique regions for probe design. The down-selected set of new or responsive probes is then synthesized and arrayed onto a separate substrate. A reserved fraction of RNA is then hybridized to the down-selected set of probes and imaged whereby the determination of the isotope incorporation into the organism using spectrometry or spectroscopy.

If a separate device to determine the isotope incorporation into the organism is not required, then a separate set of down-selected probes does not need to be made, but the determination made directly on the RNA hybridized to the larger set of probes.

These steps are meant to provide a basic process and one having skill the art should understand that optimizations and variations to the method are contemplated.

Examples of organisms that can be used in the present methods include but are not limited to, prokaryotic and eukaryotic organisms such as bacteria, archaea, fungi, plants, arthropods, nematodes, avians, mammals, and other eukaryotes, or viruses and phage. In one embodiment, the organism, multiple organisms or a community of organisms is bacteria, archaea, fungi, plants, arthropods, or nematodes. For larger organisms, a cell or tissue sample may be obtained and the RNA extracted from the sample.

The RNA extracted from the organisms may be the total RNA including ribosomal, messenger, and transfer RNA or it may be a subset of the total RNA.

The organisms are supplied with amino acids, cellulose or other labeled substrate containing a stable-isotope. Examples of such stable isotopes include but are limited to ³H, ¹³C, ¹⁵N, and/or ¹⁸O. Examples of such labeled substrate include ¹³C-amino acids, cellulose or ¹⁵NH₄ labeled substrate.

The organisms are supplied the labeled substrate for a defined period of time, such as for several minutes, hours or days. In one embodiment, a microbial community is supplied a labeled substrate for a period of 12, 18, or 24 hours.

Extraction of RNA from the organisms are generally carried out using methods known in the art. Examples of RNA extraction methods for microbial communities are provided in the Examples. In one embodiment, physical and/or chemical cell lysis and affinity column purification is used to extract RNA from the organisms or the cell or tissue sample from the organisms.

Fragmentation of the RNA is often carried out using enzymes, chemicals or heat or any combination of these. A fraction or aliquot of the fragmented RNA is labeled with a fluorescent label for suitable detection or with a label having a known binding partner to which a detectable label can be attached. In another embodiment, the fragmented RNA is labeled with a fluorescent molecule such as Alexafluor 546. In some embodiments, the fragmented RNA is labeled with biotin to which a fluorescently labeled streptavidin can be bound.

After labeling a fraction of the RNA, hybridization of the fragmented labeled RNA to a set of oligonucleotide probes is carried out. The set of oligonucleotide probes is typically attached to a solid planar substrate or on a microarray slide. However, it is contemplated that the probes may be attached to spheres, or other beads or other types of substrates. The substrates often made of materials including but not limited to, silicon, glass, metals or semiconductor materials, polymers and plastics. The substrates may be coated with other metals or materials for specific properties. In one embodiment, the substrate is coated with indium tin oxide (ITO) to provide a conductive surface for NanoSIMS analysis. The oligonucleotide probes may be present in other analysis systems, including but not limited to bead or solution multiplex reaction platforms, or across multiple platforms, for example, Affymetrix GeneChip® Arrays, Illumina BeadChip® Arrays, Luminex xMAP® Technology, Agilent Two-Channel Arrays, MAGIChips (Analysis systems of Gel-immobilized Compounds) or the NanoString nCounter Analysis System. The Affymetrix (Santa Clara, Calif., USA) platform DNA arrays can have the oligonucleotide probes (approximately 25 mer) synthesized directly on the glass surface by a photolithography method at an approximate density of 10,000 molecules per μm² (Chee et al., Science (1996) 274:610-614). Spotted DNA arrays use oligonucleotides that are synthesized individually at a predefined concentration and are applied to a chemically activated glass surface.

The oligonucleotide probes are probes generally of lengths that range from a few nucleotides to hundreds of bases in length, but are typically from about 10 mer to 50 mer, about 15 mer to 40 mer, or about 20 mer to about 30 mer in length.

In one embodiment, the oligonucleotide probes is a set of phylogenetic probes. In another embodiment, the phylogenetic probes comprising 16S rRNA phylogenetic probes. In one embodiment, the set of 16S rRNA phylogenetic probes further comprising probes from the 16S rRNA gene, 23S rRNA gene, 5S rRNA gene, 5.8S rRNA gene, 12S rRNA gene, 18S rRNA gene, 28S rRNA gene, gyrB gene, rpoB gene, fusA gene, recA gene, cox1 gene, nif13 gene, RNA molecules derived therefrom, or a combination thereof.

Features of phylogenetic microarrays of the invention include the use of multiple oligonucleotide probes for every known category of prokaryotic organisms for high-confidence detection, and the pairing of at least one mismatch probe for every perfectly matched probe to minimize the effect of nonspecific hybridization. In some embodiments, each perfect match probe corresponds to 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, or more mismatch probes. These and other features, alone or in combination as described herein, make arrays of the invention extremely sensitive, allowing identification of very low levels of microorganisms.

Methods to design and select suitable probes and arrays for Chip-SIP analysis are described in detail in co-pending U.S. patent application Ser. No. 12/474,204, filed on May 28, 2009 published as US-2009-0291858-A1, and co-pending international application having application number PCT/US2010/040106, filed on Jun. 25, 2010, both of which are incorporated by reference in their entirety for all purposes.

In one embodiment, the 16s rRNA phylogenetic probes are provided on a microarray chip, such as the G2 Phylochip or the G3 Phylochip available from Phylotech, Inc. (Second Genome, Inc., San Francisco, Calif.) and Affymetrix (Santa Clara, Calif.).

Again, the RNA that is hybridized to the probes are then imaged to detect hybridization signal strength and thereby quantify the labeled RNA to determine the community organism composition and also to correct and normalize the isotope signals in the RNA bound to each probe.

In one embodiment, for analysis for microbial composition and normalization of isotope signals, microarrays hybridized with fluorescent/biotin labeled RNA are imaged with a fluorescence scanner and fluorescence intensity measured for each probe feature or “spot”. Arrays can be scanned using any suitable scanning device. Non-limiting examples of conventional microarray scanners include GeneChip Scanner 3000 or GeneArray Scanner, (Affymetrix, Santa Clara, Calif.); and ProScan Array (Perkin Elmer, Boston, Mass.); and can be equipped with lasers having resolutions of 10 pm or finer. The scanned image displays can be captured as a pixel image, saved, and analyzed by quantifying the pixel density (intensity) of each spot on the array using image quantification software (e.g., GeneChip Analysis system Analysis Suite, version 5.1 Affymetrix, Santa Clara, Calif.; and ImaGene 6.0, Biodiscovery Inc. Los Angeles, Calif., USA). For each probe, an individual signal value can be obtained through imaging parsing and conversion to xy-coordinates. Intensity summaries for each feature can be created and variance estimations among the pixels comprising a feature can be calculated.

With flow cytometry based detection systems, a representative fraction of microparticles in each sublot of microparticles can be examined. The individual sublots, also known as subsets, can be prepared so that microparticles within a sublot are relatively homogeneous, but differ in at least one distinguishing characteristic from microparticles in any other sublot. Therefore, the sublot to which a microparticle belongs can readily be determined from different sublots using conventional flow cytometry techniques as described in U.S. Pat. No. 6,449,562. Typically, a laser is shined on individual microparticles and at least three known classification parameter values measured: forward light scatter (C₁) which generally correlates with size and refractive index; side light scatter (C₂) which generally correlates with size; and fluorescent emission in at least one wavelength (C₃) which generally results from the presence of fluorochrome incorporated into the labeled target sequence. Because microparticles from different subsets differ in at least one of the above listed classification parameters, and the classification parameters for each subset are known, a microparticle's sublot identity can be verified during flow cytometric analysis of the pool of microparticles in a single assay step and in real-time. For each sublot of microparticles representing a particular probe, the intensity of the hybridization signal can be calculated along with signal variance estimations after performing background subtraction.

In one embodiment, responsive probe-sets are then identified based on a set criteria. See FIG. 4 For example, when using the Phylochip array of probes, the responsive probe sets are identified based on probability of probe intensities originating in the positive or background intensity distributions. High confidence subfamilies identified with expected 98.4% True Positive Rate and 2.4% False Positive Rate. Probes targeting most probable taxa in high confidence subfamilies are ranked based on quality criteria such as the lowest potential for cross-hybridization across network of putatively present taxa and the greatest difference between Perfect Match (PM) and Mismatch (MM) probe intensities. Ranked PM probes plus corresponding MM probes are synthesized onto an array and then hybridized to a reserved fraction of the RNA isolated from the organism or sample.

Various methods of mass spectrometry may be used in addition to detection using the present phylogenetic probes, such as nanoSIMS (nanoscale secondary ion mass spectrometry) or time-of-flight secondary ion mass spectrometry or other methods or means of spectrometry or spectroscopy. In other embodiments, the use of spectroscopic methods that may be employed include Raman spectroscopy or reflectance or absorbance spectroscopy. In one preferred embodiment, for analysis of isotope incorporation into organisms, microarrays hybridized with non-fluorescently labeled RNA are imaged with a secondary ion mass spectrometer, such as a SIMS or NanoSIMS device. In a specific embodiment, the NanoSIMS device is a NimbleGen MAS and the probe array is synthesized onto ITO-coated slides suitable for NanoSIMS analysis.

In some embodiments, sequence information generated from reverse-transcribed RNA (cDNA) from the same samples is used to select unique regions for probe design.

In another embodiment, the array of probes is synthesized on a substrate coated with Indium Tin Oxide (ITO) to provide a conductive surface for NanoSIMS analysis. For example, ranked PM probes plus corresponding MM probes are synthesized using the NimbleGen MAS on ITO-coated slides suitable for NanoSIMS analysis.

Current and future research will focus on the cellulose-degrading and N-fixing microorganisms found in the guts of the passalid beetle Odontotaenius disjunctus. This microbial community represents a naturally-selected highly-efficient lignocellulose degrading consortium, including Pichia stipitis, a yeast with high capacity for xylose fermentation (Nardi, J. B., C. M. Bee, L. A. Miller, N. H. Nguyen, S.-O. Suh, and M. Blackwell. 2006. Arthropod Struct. Devel. 35:57-68; Suh, S.-O., J. V. McHugh, D. Pollock, and M. Blackwell. 2005. Mycolog. Res. 109:261-265). RNA from beetles have been analyzed with LBNL's Phylochip (Brodie, E. L., T. Z. DeSantis, D. C. Joyner, S. M. Baek, J. T. Larsen, G. L. Andersen, T. C. Hazen, P. M. Richardson, D. J. Herman, T. K. Tokunaga, J. M. M. Wan, and M. K. Firestone. 2006. Appl. Environ. Microbiol. 72:6288-6298) and probes are being chosen for analysis based on signal intensity relative to background.

We have demonstrated the capability of the Chip-SIP method to link phylogenetic identity and biogeochemical function. We have achieved this by incubating natural microbial communities in the presence of isotope-enriched substrates and analyzing rRNA from those communities for isotopic enrichment in a taxon-specific manner using phylogenetic microarrays. This method can be applied to all microbial systems to advance our understanding of the microorganisms involved in the sequestration of soil and marine carbon, the deconstruction of biofuel feedstocks, biodegradation of organic pollutants and bioimmobilization of radionuclides and heavy metals.

In another embodiment, the phylogenetic probes and the present methods can be used by detecting how the labeled isotope is incorporated or expressed in an organism for resource partitioning. Observing what organisms are actively consuming of a labeled substrate can provide for identifying contaminant degraders, organisms metabolizing biofuel feed stocks and soil/marine organic matter, and optimizing or monitoring biostimulation of microbes for bioremediation as further examples.

EXAMPLE 1 Applying a Chip-SIP Method to a Marine Microbial Community

To test the chip-SIP approach, we grew a single bacterial strain (Pseudomonas stutzeri) in a minimal medium with ¹³C-glucose as the sole carbon source and extracted its RNA. After fluorescent labeling, the RNA was hybridized to a microarray probe set consisting of >100 sequences targeting different regions of the P. stutzeri 16S rRNA gene. Measured isotopic enrichment of these probe spots strongly depended on the efficiency of target RNA hybridization, as quantified by fluorescence (FIGS. 1A, 1B). This correlation is the result of dilution of the target RNA isotopic signal by the background of unenriched oligonucleotide probes. Thus, if less target RNA hybridizes to the array surface, higher dilution results in a lower measured isotopic enrichment. Relative isotopic enrichment of RNA from an organism can be quantified based on the slope of the enrichment:fluorescence relationship for a single probe set. We refer to this value as the hybridization-corrected enrichment (HCE; FIG. 9).

Before applying chip-SIP to natural communities, we sought to test its sensitivity and ability to discriminate a mixture of differentially labeled bacterial taxa. Two bacterial strains, Vibrio cholerae and Bacillus cereus, were grown separately to different ¹⁵N and ¹³C isotopic enrichments, then their combined RNA was hybridized to an array consisting of probe sets targeting each organism. Both ¹³C (FIG. 2B) and ¹⁵N (FIG. 2C) enrichment can easily be distinguished for each taxon based on their respective HCEs, which were significantly different (ANCOVA; p<0.0001). By integrating the results from each organism's probe set (10-20 probes/taxon), the HCE values allow the direct comparison of isotopic incorporation between two or more taxa on a single array. Notably, we successfully detected isotopic enrichments as low as 0.5% ¹³C RNA (half of background ¹³C) and 0.1% ¹⁵N RNA (one third of background ¹⁵N), enrichment levels that traditional SIP techniques currently cannot resolve (8, 10).

EXAMPLE 2 Materials and Methods

These present example describes the materials and methods used in the Examples.

Growth of Single Strains and Incubation of Field Samples.

Strains of Pseudomonas stutzeri ATTC 11607, Vibro cholerae ATCC 14104, and Bacillus cereus D17 were grown from −80° C. frozen stock in Luria-Bertani (LB) broth at 37° C. until late log phase, and transferred into ¹²C glucose-amended M9 minimal medium until late log phase. Then, a 10 μl aliquot was inoculated into 10 ml of M9 enriched in ¹³C glucose and/or ¹⁵N ammonium and the culture was again grown until late log phase. An enrichment of 10% ¹³C indicates 10% of the glucose in the medium was 99% enriched in ¹³C, and 90% of the glucose had natural carbon (1.1% ¹³C and 98.9% ¹²C). Cells were centrifuged, washed, and frozen at −80° C. Bulk measurements (by Isotope Ratio Mass Spectrometry) showed that Pseudomonas cells grown in full ¹³C glucose were enriched between 680,000 and 900,000 permil, equivalent to 90 atm %.

For field experiments, surface water was collected at the public pier in Berkeley, Calif. USA (37°51′46.67″N, 122°19′3.23″W) and immediately brought back to the laboratory i cooler. Glass bottles (500 ml) were filled without air space and dark incubated at 14° C. For the first set of experiments, samples were simultaneously incubated with 50 μM 99 atm % ¹³C glucose and 200 μM 99 atm % ¹⁵N ammonium, and subsamples harvested after 2, 6, and 24 hrs by filtration through a 0.22 polycarbonate filter which was then immediately frozen at −80° C. Background concentrations of ammonium in San Francisco bay range from 1-14 μM (1); typically estuarine glucose concentrations are 5-100 nM (2). For the second set of experiments, water samples were incubated as described above with 8 μM mixed amino acids (99 atm % ¹³C and 99 atm % ¹⁵N labeled; Omicron), 500 μg L⁻¹ algal fatty acids (98 atm % ¹³C; Omicron), or 50 μg L⁻¹ nucleic acids (90 atm % ¹³C; RNeasy extract from ¹³C Pseudomonas stutzeri), collected by filtration after 12 hrs and frozen at −80° C. These substrate additions were designed to result in concentrations at the high end of what is typically measured in estuarine environments: 2-7 μM amino acids (3), 25 μg L⁻¹ fatty acids (4) and 10 μg L⁻¹ DNA (5).

RNA Extraction and Labeling.

RNA from pelleted cells (laboratory strains) and filters (field samples) was extracted with the Qiagen RNEasy kit according to manufacturer's instructions, with slight modifications for the field samples. Filters were incubated in 200 μL TE buffer with 5 mg mL⁻¹ lysozyme and vortexed for 10 min at RT. RLT buffer (800 μL, Qiagen) was added, vortexed, centrifuged, and the supernatant was transferred to a new tube. Ethanol (560 μl) was added, mixed gently, and the sample was applied to the provided minicolumn. The remaining manufacturer's protocol was subsequently followed. At this point, RNA samples were split: one fraction saved for fluorescent labeling (see below), the other was kept unlabeled for NanoSIMS analysis. This procedure was used because the fluorescent labeling protocol introduces background carbon (mostly ¹²C) that dilutes the ¹³C signal (data not shown). Alexafluor 546 labeling was done with the Ulysis kit (Invitrogen) for 10 min at 90° C. (2 μL RNA, 10 μL labeling buffer, 2 μL Alexafluor reagent), followed by fragmentation. All RNA (fluorescently labeled or not) was fragmented using 5× fragmentation buffer (Affymetrix) for 10 min at 90° C. before hybridization. Labeled RNA was purified using a Spin-OUT™ minicolumn (Millipore), and RNA was concentrated by ethanol precipitation to a final concentration of 500 ng μL⁻¹.

Target Taxa Selection by PhyloC Hip Analysis and De Novo Probe Design.

RNA extracts from SF Bay SIP experiment samples were treated with DNAse I and reverse-transcribed to produce cDNA using the Genechip Expression 3′ amplification one-cycle cDNA synthesis kit (Affymetrix). The cDNA was PCR amplified with bacterial and archaeal primers, fragmented, fluorescently labeled, and hybridized to the G2 PhyloChip which is described by E. L. Brodie et al., in “Application of a high-density oligonucleotide microarray approach to study bacterial population dynamics during uranium reduction and reoxidation.” Appl. Environ. Microbiol. 72, 6288 (2006) hereby incorporated by reference, and commercially available from Affymetrix (Santa Clara, Calif.) through Second Genome (San Francisco, Calif.).

Taxa (16S operating taxonomic units, OTU) considered to be present in the samples were identified based on 90% of the probes for that taxon being responsive, defined as the signal of the perfect match probe>1.3 times the signal from the mismatch probe. From approximately 1500 positively identified taxa, we chose a subset of 100 taxa commonly found in marine environments to target with chip-SIP. We also did not target OTUs previously identified from soil, sewage, and bioreactors as our goal was to characterize the activity of marine microorganisms. Using the Greengenes database (7) implemented in ARB (8), we designed 25 probes (25 bp long), to create a ‘probe set’ for each taxon (Table 1; SEQ ID NOS: 1-2805), as well as general probes for the three domains of life. Probes for single laboratory strains (Pseudomonas stutzeri, Bacillus cereus, and Vibrio cholerae) were also designed with ARB (Table 1).

Microarray Synthesis and Hybridization.

A custom conductive surface for the microarrays was used to eliminate charging during SIMS analysis. Glass slides coated with indium-tin oxide (ITO; Sigma) were treated with an alkyl phosphonate hydroxy-linker (patent pending) to provide a starting substrate for DNA synthesis. Custom-designed microarrays (spot size=17 μm) were synthesized using a photolabile deprotection strategy (9) on the LLNL Maskless Array Synthesizer (Roche Nimblegen, Madison, Wis.). Reagents for synthesis (Roche Nimblegen) were delivered through the Expedite (PerSeptive Biosystems) system. For quality control (to determine that DNA synthesis was successful), slides were hybridized with complimentary Arabidopsis calmodulin protein kinase 6 (CPK6) labeled with Cy3 (Integrated DNA Technologies), which hybridized to fiducial marks, probe spots with the complementary sequence synthesized throughout the array area. Probes targeting microbial taxa were arranged in a densely packed formation to decrease the total area analyzed by imaging secondary ion mass spectrometry (NanoSIMS). For array hybridization, RNA samples in 1× Hybridization buffer (NimbleGen) were placed in Nimblegen X4 mixer slides and incubated inside a Maui hybridization system (BioMicro® Systems) for 18 hrs at 42° C. and subsequently washed according to manufacturer's instructions (NimbleGen). Arrays with fluorescently labeled RNA were imaged with a Genepix 4000B fluorescence scanner at pmt=650 units. Arrays with non-fluorescently labeled RNA were marked with a diamond pen and also imaged with the fluorescence scanner to subsequently navigate to the analysis spots in the NanoSIMS. These spots were observable in the fluorescence image because fiducial probe spots were synthesized around the outline of the area to be analyzed by NanoSIMS. Prior to NanoSIMS analysis, samples were not metal coated to avoid further dilution of the RNA's isotope ratio or loss of material. Slides were trimmed and mounted in custom-built stainless steel holders.

NanoSIMS Analyses.

Secondary ion mass spectrometry analysis of microarrays hybridized with ¹³C and/or ¹⁵N rRNA was performed at LLNL with a Cameca NanoSIMS 50 (Cameca, Gennevilliers, France). A Cs+ primary ion beam was used to enhance the generation of negative secondary ions. Carbon and nitrogen isotopic ratios were determined by electrostatic peak switching on electron multipliers in pulse counting mode, alternately measuring ¹²C¹⁴N⁻ and ¹²C¹⁵N⁻ simultaneously for the ¹⁵N/¹⁴N ratio, and then simultaneously measuring ¹²C¹⁴N⁻ and ¹³C¹⁴N⁻ for the ¹³C/¹²C ratio. We used this peak switching strategy because the secondary ion count rate for the CN⁻ species in these samples is 5-10 times higher than any of the other carbon species (e.g., C⁻, CH⁻, C₂ ⁻), and therefore higher precision was achieved even though total analytical time was split between the two CN⁻ species at mass 27. If only one isotopic ratio was needed, peak switching was not performed. Mass resolution was set to ˜10,000 mass resolving power to minimize the contribution of isobaric interferences to the species of interest (e.g., ¹¹B¹⁶O⁻ contribution to ¹³C¹⁴N⁻< 1/100; ¹³C₂ ⁻ contribution to ¹²C¹⁴N⁻< 1/1000). Analyses were performed in imaging mode to generate digital ion images of the sample for each ion species. Analytical conditions were optimized for speed of analysis, ability to spatially resolve adjacent hybridization locations, and analytical stability. The primary beam current was 5 to 7 pA Cs⁺, which yielded a spatial resolution of 200-400 nm and a maximum count rate on the detectors of ˜300,000 cps ¹²C¹⁴N. Analysis area was 50×50 μm² with a pixel density of 256×256 with 0.5 or 1 ms/pixel dwell time. For peak switching, one scan of the analysis area was made per species set, resulting in two scans per analytical cycle. With these conditions, reproducible secondary ion ratios could be measured for a maximum of 4 cycles through the two sets of measurements before the sample was largely consumed. Data were collected for 2 to 4 cycles. Based on total counts for the analyzed cycles, we achieved precision of 2-3% for ¹³C¹⁴N and 1-4% for ¹⁵N¹²C, depending on the enrichment and hybridization intensity. A single microarray analysis of approximately 2500 probes, with an area of 0.75 mm² and the acquisition of 300 images, was carried out using the Cameca software automated chain analysis in 16 hours. Ion images were stitched together and processed to generate isotopic ratios with custom software (LIMAGE, L. Nittler, Carnegie Institution of Washington). Ion counts were corrected for detector dead time on a pixel by pixel basis. Hybridization locations were selected by hand or with the auto-ROI function, and ratios were calculated for the selected regions over all cycles to produce the location isotopic ratios. Isotopic ratios were converted to delta values using δ=[(R_(meas)/R_(standard))−1]×1000, where R_(meas) is the measured ratio and R_(standard) is the standard ratio (0.00367 for ¹⁵N/¹⁴N and 0.011237 for ¹³C/¹²C). Data were corrected for natural abundance ratios measured in unhybridized locations of the sample.

Data Analyses.

For each taxon, isotopic enrichment of individual probe spots was plotted against fluorescence and the linear regression slope was calculated with the y-intercept constrained to natural isotope abundances (zero permil for ¹⁵N data and −20 permil for ¹³C data). This calculated slope (permil/fluorescence), which we refer to as the ‘hybridization-corrected enrichment (HCE), is a metric that can be used to compare the relative incorporation of a given substrate by different taxa. It should be noted that due to the different natural concentrations of ¹³C and ¹⁵N, and more importantly, different background contributions from the microarray, HCEs for ¹⁵N substrates and ¹³C substrates are not comparable. To construct a network diagram (e.g. FIG. 8A), taxa with HCEs having standard errors not overlapping with zero and with >30 permil enrichment were included (all others were discarded) using Cytoscape software (10). For analyses of marine bacterial genomic information, genomes of marine bacterial isolates were selected in the Joint Genome Institute's Integrated Microbial Genomes (IM-G) database and word-searched for the presence of amino acid, fatty acid, and nucleoside transporters and extracellular nucleases. Results are summarized in Table S2. For phylogenetic relationships (FIG. 8B), the global 16S rRNA phylogeny in the Greengenes database (7) was opened in ARB (8) and all taxa except the targets of the array analysis were removed with the taxon pruning function.

EXAMPLE 3 Viability of Chip-SIP on a San Francisco Bay Sample

In a second set of experiments, we tested the viability of chip-SIP for a diverse natural community, using a sample from the San Francisco (SF) Bay, a eutrophic estuary. The bay water was incubated in the dark with micromolar concentrations of ¹⁵N ammonium and ¹³C glucose for 24 hrs, a timescale long enough to ensure detectable isotopic labeling of the dominant active community. We expected the most active taxa to incorporate these substrates, as they are of small molecular weight, do not require extracellular breakdown before uptake, and directly feed into central carbon and nitrogen metabolic pathways. This chip-SIP array consisted of 2500 probes targeting 100 microbial taxa selected from a PhyloChip analysis of the same sample (Table 1; 16). Based on RNA fluorescence, we positively detected 73 taxa. As in the experiments with laboratory cultures, the relationship between fluorescence and isotopic incorporation for each taxon was positive and linear for both ¹⁵N and ¹³C (e.g. FIGS. 7A, 7B for three Rhodobacteraceae probes sets), demonstrating that different members of the same bacterial families could incorporate different levels of ¹⁵N from ammonium and ¹³C from glucose. Though these substrate concentrations may have favored copiotrophs (17), we detected the model oligotroph Pelagibacter (FIGS. 10, 18). This result demonstrates that even oligotrophic organisms retained a presence and detectable biogeochemical activity in this highly eutrophic environment.

An advantage of chip-SIP's ability to detect ¹³C and ¹⁵N on the same array is its potential to uncover physiological diversity, based on the relative incorporation of two substrates incubated simultaneously. Our ability to measure taxon-specific substrate incorporation allowed us to reveal that the relationship between ammonium and glucose incorporation was linear: organisms with high ammonium incorporation (high ¹⁵N HCEs) also exhibited high glucose incorporation (high ¹³C HCEs), and vice versa (FIGS. 7C, 10). A previous experiment illustrated an analogous pattern using lower resolution bulk measurements comparing marine water samples amended with different levels of glucose and ammonium(19). The authors found that community wide C/N assimilation was constant, irrespective of the absolute amount of substrates added. Our data revealed that this pattern also occurs within the same water sample, in which different microbial populations represent physiologically distinct components of the community. We also showed that relatively broad phylogenetic clades (family level, in this case) did not correspond to substrate incorporation patterns: members of the same bacterial family were scattered throughout the HCE distribution. For example, members of the Flavobacteriaceae did not incorporate less (or more) substrate than the Rhodobacteraceae, because within each family, there were taxa with both high and low incorporation (FIG. 7C).

EXAMPLE 4 Viability of Chip-SIP on a San Francisco Bay Sample

Marine microorganisms, most of which remain uncultivated, control the release, transformation, and remineralization of ˜50 Gigatons of fixed carbon annually, resulting in biological carbon sequestration to the deep sea (P. Falkowski et al., The global carbon cycle: a test of our knowledge of earth as a system. Science 290, 291 (2000)). Identifying the microbes responsible for C cycling processes in the marine microbial loop and the factors affecting C cycling rates in marine ecosystems is a critical precursor to the development of predictive models of microbial responses to environmental perturbations (e.g., pollution, nutrient inputs or global change). Currently, the ecological niches of marine microorganisms, heterotrophic bacteria in particular, are often categorized as “copiotrophic” or “oligotrophic” depending on their predominant location, for example high-nutrient and particle-rich coasts versus low-nutrient open oceans, or warm, well-lit, productive surface waters versus the cold, dark deep (S. J. Giovannoni, U. Stingl, Molecular diversity and ecology of microbial plankton. Nature 437, 343 (2005)). The advent of 16S rRNA sequencing and environmental genomics have revolutionized marine microbial ecology by assembling a “parts list” of genetic diversity (M. S. Rappé, P. F. Kemp, S. J. Giovannoni, Phylogenetic diversity of marine coastal picoplankton 16S rRNA genes cloned from the continental shalf off Cape Hatteras, N.C. Limnol. Oceanogr. 42, 811 (1997)) and functional capability (E. F. DeLong et al., Community genomics among stratified microbial assemblages in the ocean's Interior. Science 311, 496 (2006)), but the goal of linking phylogenetic identity and in situ functional roles of uncultivated microorganisms remains largely unattained. In addition, while the comparative ‘omics strategy to gain ecosystem functional information has been fruitful, it relies on sequence comparison rather than direct measurements of biogeochemical activity. To gain a mechanistic understanding of microbial control of biogeochemical cycles in the ocean and elsewhere, it is necessary to move beyond microbial diversity or metagenomic surveys towards trait-based functional studies that directly and simultaneously measure the biogeochemical activities of hundreds of microbial taxa in their native environment.

In a third set of experiments, we compared predicted and actual substrate use of three organic substrates by a diverse natural community, an example of the type of experiment that can eventually lead to more realistic models of marine food web structure (20). In this case, we applied chip-SIP to another set of SF Bay samples incubated separately with isotopically-labeled amino acids, nucleic acids, and fatty acids. These substrates make up a significant proportion of photoautotrophic biomass (21) that provide the majority of fixed carbon substrates for the marine microbial food web.

We detected isotopic enrichment of at least one of the three added substrates in 52 out of the 81 taxa with positive RNA hybridization (FIGS. 10-13). A network diagram, based on the measured HCE values, illustrates the movement of organic matter between substrates and microbial taxa, and clearly indicates generalists that incorporated all three substrates versus specialist consumers of only one substrate (FIG. 8A). Our analysis reveals that generalists and specialists were not necessarily distinguishable based on 16S phylogeny. In other words, members of a bacterial family could be generalists while others specialists. Such an analysis, which includes quantitative information (visualized by the thickness of the lines connecting substrates to taxa), is a substantial step forward in our understanding of organic matter flow in the microbial loop.

To compare genome-predicted potential biogeochemical activity to our measured substrate incorporation data, we examined the presence of genes involved in the extracellular degradation or transport of these substrates in the sequenced genomes of marine bacterial isolates (Table 2). Table 2 is shown below:

TABLE S2 presence of identified amino acid transporters, extracellular nucleases, and nucleoside and fatty acid transport in 110 genomes of marine bacterial isolates. Word searches performed with Joint Genome Institute's Integrated Microbial Genomes (IMG) online at IMG JGI website. Amino acid Extracellular Nucleoside Fatty acid Genome transport nuclease transport transport Agreia sp. PHSC20C1 Y N N N Algoriphagus sp. PR1 Y N Y Y Aurantimonas sp. SI85-9A1 Y N N N Bacillus sp. B14905 Y N Y N Bacillus sp. NRRL B-14911 Y N Y N Bacillus sp. SG-1 Y Y Y N Beggiatoa sp. PS Y N N Y Bermanella marisrubri Y N N Y Blastopirellula marina DSM 3645 Y Y N N Caminibacter mediatlanticus TB-2 Y N N N Candidatus Blochmannia Y N N N pennsylvanicus BPEN Candidatus Pelagibacter ubique Y N N N HTCC1002 Carnobacterium sp. AT7 Y N Y Y Congregibacter litoralis KT71 Y N Y N Croceibacter atlanticus HTCC2559 Y Y Y N Cyanothece sp. CCY 0110 Y N Y N Dokdonia donghaensis MED134 Y Y Y N Erythrobacter litoralis HTCC2594 Y N Y Y Erythrobacter sp. NAP1 Y N N N Erythrobacter sp. SD-21 Y N Y N Finegoldia magna ATCC 29328 Y N N N Flavobacteria bacterium BAL38 Y N N Y Flavobacteria bacterium BBFL7 N Y N N Flavobacteriales bacterium ALC-1 Y N Y N Flavobacteriales bacterium HTCC2170 Y N Y N Fulvimarina pelagi HTCC2506 Y N N Y Hoeflea phototrophica DFL-43 Y N N Y Hydrogenivirga sp. 128-5-R1-1 Y N N N Idiomarina baltica OS145 Y Y N Y Janibacter sp. HTCC2649 Y Y N N Kordia algicida OT-1 Y Y N Y Labrenzia aggregata IAM 12614 Y N N N Leeuwenhoekiella blandensis MED217 Y N Y N Lentisphaera araneosa HTCC2155 Y N N Y Limnobacter sp. MED105 Y N N Y Loktanella vestfoldensis SKA53 Y N N N Lyngbya sp. PCC 8106 Y Y Y N marine gamma proteobacterium Y Y Y Y HTCC2080 marine gamma proteobacterium Y N N N HTCC2143 marine gamma proteobacterium Y N N N HTCC2148 marine gamma proteobacterium Y N N N HTCC2207 Marinobacter algicola DG893 Y Y N Y Marinobacter sp. ELB17 Y N N Y Marinomonas sp. MED121 Y Y N N Mariprofundus ferrooxydans PV-1 Y N N Y Methylophilales bacterium HTCC2181 N N N N Microscilla marina ATCC 23134 Y Y Y N Moritella sp. PE36 Y Y Y Y Neptuniibacter caesariensis Y N N N Nisaea sp. BAL199 Y N N Y Nitrobacter sp. Nb-311A Y N N N Nitrococcus mobilis Nb-231 Y N N N Nodularia spumigena CCY9414 Y N N N Oceanibulbus indolifex HEL-45 Y N Y Y Oceanicaulis alexandrii HTCC2633 Y N N N Oceanicola batsensis HTCC2597 Y Y N N Oceanicola granulosus HTCC2516 Y Y Y N Parvularcula bermudensis HTCC2503 Y Y Y N Pedobacter sp. BAL39 Y N N Y Pelotomaculum thermopropionicum SI Y N N N Phaeobacter gallaeciensis 2.10 Y N N N Phaeobacter gallaeciensis BS107 Y N N N Photobacterium angustum S14 Y Y Y Y Photobacterium profundum 3TCK Y Y Y Y Photobacterium sp. SKA34 Y Y Y Y Planctomyces maris DSM 8797 Y Y N N Plesiocystis pacifica SIR-1 Y N Y Y Polaribacter irgensii 23-P Y Y Y N Polaribacter sp. MED152 Y Y Y N Prochlorococcus marinus AS9601 N N N N Prochlorococcus marinus MIT 9211 Y N N N Prochlorococcus marinus MIT 9301 N N N N Prochlorococcus marinus MIT 9303 Y N N N Prochlorococcus marinus MIT 9515 Y N N N Prochlorococcus marinus NATL1A Y N N N Pseudoalteromonas sp. TW-7 Y N Y Y Pseudoalteromonas tunicata D2 Y N Y Y Psychroflexus torquis ATCC 700755 Y Y N N Psychromonas sp. CNPT3 Y N N Y Reinekea sp. MED297 Y Y N N Rhodobacterales bacterium HTCC2150 Y Y Y N Rhodobacterales bacterium HTCC2654 Y N N N Rhodobacterales sp. HTCC2255 Y N Y N Roseobacter litoralis Och 149 Y N N N Roseobacter sp. AzwK-3b Y N N N Roseobacter sp. CCS2 Y Y N N Roseobacter sp. MED193 Y N N N Roseobacter sp. SK209-2-6 Y N Y N Roseovarius nubinhibens ISM Y N N N Roseovarius sp. 217 Y Y Y Y Roseovarius sp. HTCC2601 Y N Y N Roseovarius sp. TM1035 Y N N N Sagittula stellata E-37 Y Y N N Shewanella benthica KT99 Y Y N Y Sphingomonas sp. SKA58 Y N Y Y Sulfitobacter sp. EE-36 Y N N N Sulfitobacter sp. NAS-14.1 Y N N N Synechococcus sp. BL107 Y N N N Synechococcus sp. RS9916 Y N N N Synechococcus sp. RS9917 Y N N N Synechococcus sp. WH 5701 Y N N N Synechococcus sp. WH 7805 Y N N N Ulvibacter sp. SCB49 Y Y N Y Vibrio alginolyticus 12G01 Y Y Y Y Vibrio campbellii AND4 Y N Y Y Vibrio harveyi HY01 Y N Y Y Vibrio shilonii AK1 Y Y Y Y Vibrio sp. MED222 Y Y Y Y Vibrio splendidus 12B01 Y Y Y Y Vibrionales bacterium SWAT-3 Y Y Y Y

Incorporation of leucine and other amino acids is routinely used as a proxy for bacterial production in aquatic systems (22) and metatranscriptomic evidence suggests most marine bacterial taxa incorporate amino acids directly (23). As nearly all genomes of marine bacteria (106/110) possess annotated putative amino acid transporters, we expected most of the active microbes in the SF Bay system would incorporate amino acids. Bacterial uptake of single nucleosides (e.g. thymidine) is ubiquitous and used to measure rates of growth (24), but only a few studies have examined longer nucleic acid molecules as a source of carbon or nitrogen for microbial metabolism (see ref. 25 as a recent example). Considering that half (55/110) of fully sequenced marine bacterial genomes contain at least one identified nucleoside transporter or extracellular nuclease, we expected nucleic acid incorporation could be a common phenomenon in the environment. Finally, we also chose to examine fatty acid incorporation because marine bacterial isolates commonly reveal high lipase activity (26), although only 38/110 sequenced bacterial genomes contained identified lipid transporters. In addition, comparative genomics has shown that oligotrophic marine bacterial genomes contain significantly more genes for lipid metabolism and fatty acid degradation than copiotrophic genomes (27). If oligotrophs favor fatty acid incorporation, we hypothesized that it would be less common than amino acid incorporation in our samples since a eutrophic estuary should favor copiotrophs.

In general terms, our results agree with predictions made from available marine genomic data: amino acids were the most commonly incorporated (46 taxa), followed by nucleic acids (32 taxa) and then fatty acids (18 taxa). However, the chip-SIP and genomic data did not always concur. For example, all the Vibrio genomes we examined contain putative enzymes for the utilization of the three substrates tested (Table 2), yet chip-SIP indicates the Vibrio taxa we detected incorporated only amino acids (FIG. 8A). In this case, genomic potential did not indicate activity. With a relatively high level of taxonomic detail, chip-SIP showed that over 10% of the active taxa in this sample (6 out of 52) did not incorporate amino acid-derived ¹⁵N into their RNA, even though amino acids are considered a ubiquitous substrate for marine bacteria (22, 23). Indeed, if rates of marine bacterial carbon production based on leucine incorporation are underestimates, this could have significant implications for global carbon modeling efforts. Our analyses also revealed that bacteria commonly incorporate carbon (and presumably nitrogen) from external nucleic acid sources. This complements previous work that identified nucleic acids as a source of phosphorus for marine bacteria, (28). Nucleic acids have C/N ratios lower than phytoplankton-derived organic matter and most amino acids (average C/N of RNA=2.5, POM=6.6, amino acids=3.6). This makes them an ideal resource for bacteria that have relatively high nitrogen requirements. Fatty acids, which contain no nitrogen, were less commonly incorporated than either amino acids or nucleic acids, although we did identify one taxon (uncultivated Alphaproteobacterial clade NAC1-6) that incorporated this substrate but not the others. Such measurements of taxon-specific substrate incorporation within complex communities, along with data gleaned from genomic sequencing, could clearly be useful during the selection of strategies for isolation of previously uncultured microbial taxa.

A frequently accepted, although increasingly controversial view in microbial ecology (29), maintains that 16S phylogeny is closely related to functional role. It is widely assumed that taxa that are closely related by 16S phylogeny are more likely to be functionally similar than to taxa more phylogenetically distant. This concept has been a major assumption of microbial ecology research, without which 16S diversity surveys lose their functional context. Chip-SIP allowed us to test this assumption by matching functional in situ resource use to 16S phylogenetic relationships.

As an example, we mapped substrate utilization data across a subset of the Gammaproteobacterial phylogeny (FIG. 8B) and observed taxon specific responses. For the well characterized and previously cultivated copiotrophic organisms of the genera Vibrio and Alteromonas, patterns of resource use matched 16S phylogeny quite well: all taxa incorporated amino acids, and several Alteromonas taxa incorporated nucleic acids, while no taxon incorporated fatty acids (FIG. 8B). In this case, 16S based phylogeny correlates with resource use. However, in other phylogenetic groups there is a clear decoupling between phylogeny and biogeochemical function. The three taxa identified from the Oleispira group exhibited completely different substrate incorporation patterns (FIG. 8B): one incorporated amino acids and fatty acids, the second incorporated only nucleic acids, while the third incorporated both fatty acids and nucleic acids. Based on these data, it would be impossible to predict the resource use of a different Oleispira taxon. This decoupling between phylogenetic similarity and measured substrate incorporation illustrates the limitation of using 16S phylogenetic information to predict functional resource utilization.

Based on the success of these initial experiments, chip-SIP may facilitate great strides in our understanding of the functional mechanisms that underlie patterns of microbial diversity. Using this high resolution, high-sensitivity approach, we have revealed patterns of resource utilization in an estuarine community with critical implications for our understanding of carbon cycling in marine environments. These data considerably expand upon previous studies that have identified marine bacterial resource partitioning based on seasonal and small-scale spatial habitat use (30) by adding relative rates of substrate utilization as a critical component of the bacterial niche.

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REFERENCES FOR EXAMPLE 2

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The above examples are provided to illustrate the invention but not to limit its scope. Other variants of the invention will be readily apparent to one of ordinary skill in the art and are encompassed by the appended claims. All publications, databases, and patents cited herein are hereby incorporated by reference for all purposes.

TABLE 1 list of probes specific for laboratory bacterial strains and San Francisco Bay natural community SEQUENCE_ID PROBE_SEQUENCE SEQUENCE_ID PROBE_SEQUENCE SEQUENCE_ID  PROBE SEQUENCE Pstutzeri_1 TAACCGTCCCCCCGAAGGTTAGACT Vcholerae_1 AACTTAACCACCTTCCTCCCTACTG Bcereus_1 TCCACCTCGCGGTCTTGCAGCTCTT Pstutzeri_2 GGTAACCGTCCCCCCGAAGGTTAGA Vcholerae_2 GTAGGTAACGTCAAATGATTAAGGT Bcereus_2 GCCTTTCAATTTCGAACCATGCGGT Pstutzeri_3 TGGTAACCGTCCCCCCGAAGGTTAG Vcholerae_3 TGTAGGTAACGTCAAATGATTAAGG Bcereus_3 CTCTTAATCCATTCGCTCGACTTGC Pstutzeri_4 GTAACCGTCCCCCCGAAGGTTAGAC Vcholerae_4 TAACTTAACCACCTTCCTCCCTACT Bcereus_4 CCACCTCGCGGTCTTGCAGCTCTTT Pstutzeri_5 ACTCCGTGGTAACCGTCCCCCCGAA Vcholerae_5 ACTTAACCACCTTCCTCCCTACTGA Bcereus_5 CTCTGCTCCCGAAGGAGAAGCCCTA Pstutzeri_6 CACTCCGTGGTAACCGTCCCCCCGA Vcholerae_6 TTAACTTAACCACCTTCCTCCCTAC Bcereus_6 CCGCCTTTCAATTTCGAACCATGCG Pstutzeri_7 TCACTCCGTGGTAACCGTCCCCCCG Vcholerae_7 TAAGGTATTAACTTAACCACCTTCC Bcereus_7 TCTGCTCCCGAAGGAGAAGCCCTAT Pstutzeri_8 ACCGTCCCCCCGAAGGTTAGACTAG Vcholerae_8 CTGTAGGTAACGTCAAATGATTAAG Bcereus_8 ACCTGTCACTCTGCTCCCGAAGGAG Pstutzeri_9 ATCACTCCGTGGTAACCGTCCCCCC Vcholerae_9 CTTAACCACCTTCCTCCCTACTGAA Bcereus_9 GCTCTTAATCCATTCGCTCGACTTG Pstutzeri_10 CCGTGGTAACCGTCCCCCCGAAGGT Vcholerae_10 ATTAACTTAACCACCTTCCTCCCTA Bcereus_10 CGCCTTTCAATTTCGAACCATGCGG Pstutzeri_11 CTCCGTGGTAACCGTCCCCCCGAAG Vcholerae_11 AAGGTATTAACTTAACCACCTTCCT Bcereus_11 ACTCTGCTCCCGAAGGAGAAGCCCT Pstutzeri_12 CCGTCCCCCCGAAGGTTAGACTAGC Vcholerae_12 TTAACCACCTTCCTCCCTACTGAAA Bcereus_12 GCTCCCGAAGGAGAAGCCCTATCTC Pstutzeri_13 CCACCACCCTCTGCCATACTCTAGC Vcholerae_13 CTTCTGTAGGTAACGTCAAATGATT Bcereus_13 TCACTCTGCTCCCGAAGGAGAAGCC Pstutzeri_14 TCCACCACCCTCTGCCATACTCTAG Vcholerae_14 TATTAACTTAACCACCTTCCTCCCT Bcereus_14 TCTTAATCCATTCGCTCGACTTGCA Pstutzeri_15 TTCCACCACCCTCTGCCATACTCTA Vcholerae_15 ACGACGTACTTTGTGAGATTCGCTC Bcereus_15 CTGCTCCCGAAGGAGAAGCCCTATC Pstutzeri_16 AATTCCACCACCCTCTGCCATACTC Vcholerae_16 TACGACGTACTTTGTGAGATTCGCT Bcereus_16 TAATCCATTCGCTCGACTTGCATGT Pstutzeri_17 AAATTCCACCACCCTCTGCCATACT Vcholerae_17 ACTACGACGTACTTTGTGAGATTCG Bcereus_17 CACTCTGCTCCCGAAGGAGAAGCCC Pstutzeri_18 GAAATTCCACCACCCTCTGCCATAC Vcholerae_18 CTACGACGTACTTTGTGAGATTCGC Bcereus_18 GGTCTTGCAGCTCTTTGTACCGTCC Pstutzeri_19 ATTCCACCACCCTCTGCCATACTCT Vcholerae_19 GACTACGACGTACTTTGTGAGATTC Bcereus_19 TGCTCCCGAAGGAGAAGCCCTATCT Pstutzeri_20 GGAAATTCCACCACCCTCTGCCATA Vcholerae_20 AGGTATTAACTTAACCACCTTCCTC Bcereus_20 CTTAATCCATTCGCTCGACTTGCAT Pstutzeri_21 CAGGAAATTCCACCACCCTCTGCCA Vcholerae_21 GGTATTAACTTAACCACCTTCCTCC Bcereus_21 TTAATCCATTCGCTCGACTTGCATG Pstutzeri_22 AGGAAATTCCACCACCCTCTGCCAT Vcholerae_22 GTATTAACTTAACCACCTTCCTCCC Bcereus_22 CTCCCGAAGGAGAAGCCCTATCTCT Pstutzeri_23 CAGTGTCAGTATTAGCCCAGGTGGT Vcholerae_23 CGCGGTATCGCTGCCCTCTGTATAC Bcereus_23 GTCACTCTGCTCCCGAAGGAGAAGC Pstutzeri_24 TCAGTATTAGCCCAGGTGGTCGCCT Vcholerae_24 TCGCGGTATCGCTGCCCTCTGTATA Bcereus_24 CACCTCGCGGTCTTGCAGCTCTTTG Pstutzeri_25 TCAGTGTCAGTATTAGCCCAGGTGG Vcholerae_25 CTTGTCAGTTTCAAATGCGATTCCT Bcereus_25 GTCTTGCAGCTCTTTGTACCGTCCA Pstutzeri_26 TGTCAGTATTAGCCCAGGTGGTCGC Vcholerae_26 TTGTCAGTTTCAAATGCGATTCCTA Bcereus_26 TGTCACTCTGCTCCCGAAGGAGAAG Pstutzeri_27 GTCAGTATTAGCCCAGGTGGTCGCC Vcholerae_27 GCGGTATCGCTGCCCTCTGTATACG Bcereus_27 TCCCGAAGGAGAAGCCCTATCTCTA Pstutzeri_28 CCTCAGTGTCAGTATTAGCCCAGGT Vcholerae_28 CCTGGGCATATCCGGTAGCGCAAGG Bcereus_28 CGGTCTTGCAGCTCTTTGTACCGTC Pstutzeri_29 CTCAGTGTCAGTATTAGCCCAGGTG Vcholerae_29 TCCCACCTGGGCATATCCGGTAGCG Bcereus_29 TCAAAATGTTATCCGGTATTAGCCC Pstutzeri_30 ACCTCAGTGTCAGTATTAGCCCAGG Vcholerae_30 GGCATATCCGGTAGCGCAAGGCCCG Bcereus_30 CCTGTCACTCTGCTCCCGAAGGAGA Pstutzeri_31 GTGTCAGTATTAGCCCAGGTGGTCG Vcholerae_31 ACCTGGGCATATCCGGTAGCGCAAG Bcereus_31 TTCAAAATGTTATCCGGTATTAGCC Pstutzeri_32 AGTGTCAGTATTAGCCCAGGTGGTC Vcholerae_32 CTGGGCATATCCGGTAGCGCAAGGC Bcereus_32 CACCTGTCACTCTGCTCCCGAAGGA Pstutzeri_33 CACCTCAGTGTCAGTATTAGCCCAG Vcholerae_33 CCCACCTGGGCATATCCGGTAGCGC Bcereus_33 TCTTGCAGCTCTTTGTACCGTCCAT Pstutzeri_34 GCACCTCAGTGTCAGTATTAGCCCA Vcholerae_34 TGGGCATATCCGGTAGCGCAAGGCC Bcereus_34 CTGTCACTCTGCTCCCGAAGGAGAA Pstutzeri_35 CGCACCTCAGTGTCAGTATTAGCCC Vcholerae_35 GGGCATATCCGGTAGCGCAAGGCCC Bcereus_35 GCGGTCTTGCAGCTCTTTGTACCGT Pstutzeri_36 TTCGCACCTCAGTGTCAGTATTAGC Vcholerae_36 GCATATCCGGTAGCGCAAGGCCCGA Bcereus_36 CGCGGTCTTGCAGCTCTTTGTACCG Pstutzeri_37 TCGCACCTCAGTGTCAGTATTAGCC Vcholerae_37 CCACCTGGGCATATCCGGTAGCGCA Bcereus_37 AGCTCTTAATCCATTCGCTCGACTT Pstutzeri_38 AATGCGTTAGCTGCGCCACTAAGAT Vcholerae_38 CATATCCGGTAGCGCAAGGCCCGAA Bcereus_38 ACCTCGCGGTCTTGCAGCTCTTTGT Pstutzeri_39 CACCACCCTCTGCCATACTCTAGCT Vcholerae_39 CACCTGGGCATATCCGGTAGCGCAA Bcereus_39 TCGCGGTCTTGCAGCTCTTTGTACC Pstutzeri_40 ACACAGGAAATTCCACCACCCTCTG Vcholerae_40 ATATCCGGTAGCGCAAGGCCCGAAG Bcereus_40 CTCGCGGTCTTGCAGCTCTTTGTAC Pstutzeri_41 CACAGGAAATTCCACCACCCTCTGC Vcholerae_41 TATCCGGTAGCGCAAGGCCCGAAGG Bcereus_41 TGCACCACCTGTCACTCTGCTCCCG Pstutzeri_42 ACAGGAAATTCCACCACCCTCTGCC Vcholerae_42 TCCCCTGCTTTGCTCTTGCGAGGTT Bcereus_42 ATGCACCACCTGTCACTCTGCTCCC Pstutzeri_43 GAAGTTAGCCGGTGCTTATTCTGTC Vcholerae_43 GTCCCCTGCTTTGCTCTTGCGAGGT Bcereus_43 ACCACCTGTCACTCTGCTCCCGAAG Pstutzeri_44 GAAAGTTCTCTGCATGTCAAGGCCT Vcholerae_44 CCGAAGGTCCCCTGCTTTGCTCTTG Bcereus_44 GCACCACCTGTCACTCTGCTCCCGA Pstutzeri_45 AAAGTTCTCTGCATGTCAAGGCCTG Vcholerae_45 GGTCCCCTGCTTTGCTCTTGCGAGG Bcereus_45 CACCACCTGTCACTCTGCTCCCGAA Pstutzeri_46 TCTCTGCATGTCAAGGCCTGGTAAG Vcholerae_46 GAAGGTCCCCTGCTTTGCTCTTGCG Bcereus_46 CATAAGAGCAAGCTCTTAATCCATT Pstutzeri_47 GTTCTCTGCATGTCAAGGCCTGGTA Vcholerae_47 AGGTCCCCTGCTTTGCTCTTGCGAG Bcereus_47 CCTCGCGGTCTTGCAGCTCTTTGTA Pstutzeri_48 AGTTCTCTGCATGTCAAGGCCTGGT Vcholerae_48 CGAAGGTCCCCTGCTTTGCTCTTGC Bcereus_48 CCACCTGTCACTCTGCTCCCGAAGG Pstutzeri_49 AAGTTCTCTGCATGTCAAGGCCTGG Vcholerae_49 AAGGTCCCCTGCTTTGCTCTTGCGA Bcereus_49 AAGAGCAAGCTCTTAATCCATTCGC Pstutzeri_50 CTCTGCATGTCAAGGCCTGGTAAGG Vcholerae_50 CCCCTGCTTTGCTCTTGCGAGGTTA Bcereus_50 CGAAGGAGAAGCCCTATCTCTAGGG Pstutzeri_51 TTCTCTGCATGTCAAGGCCTGGTAA Vcholerae_51 TCTAGGGCACAACCTCCAAGTAGAC Bcereus_51 AAGCTCTTAATCCATTCGCTCGACT Pstutzeri_52 CTGCATGTCAAGGCCTGGTAAGGTT Vcholerae_52 CTCTAGGGCACAACCTCCAAGTAGA Bcereus_52 TAAGAGCAAGCTCTTAATCCATTCG Pstutzeri_53 TCTGCATGTCAAGGCCTGGTAAGGT Vcholerae_53 CCTCTAGGGCACAACCTCCAAGTAG Bcereus_53 ATAAGAGCAAGCTCTTAATCCATTC Pstutzeri_54 TACTCACCCGTCCGCCGCTGAATCA Vcholerae_54 CGACGTACTTTGTGAGATTCGCTCC Bcereus_54 CCCGAAGGAGAAGCCCTATCTCTAG Pstutzeri_55 CAGCCATGCAGCACCTGTGTCAGAG Vcholerae_55 TCAGTTTCAAATGCGATTCCTAGGT Bcereus_55 CCGAAGGAGAAGCCCTATCTCTAGG Pstutzeri_56 ACAGCCATGCAGCACCTGTGTCAGA Vcholerae_56 AGTTTCAAATGCGATTCCTAGGTTG Bcereus_56 CAAGCTCTTAATCCATTCGCTCGAC Pstutzeri_57 GACAGCCATGCAGCACCTGTGTCAG Vcholerae_57 TGTCAGTTTCAAATGCGATTCCTAG Bcereus_57 AAGGAGAAGCCCTATCTCTAGGGTT Pstutzeri_58 CTGGAAAGTTCTCTGCATGTCAAGG Vcholerae_58 GTTTCAAATGCGATTCCTAGGTTGA Bcereus_58 GAAGGAGAAGCCCTATCTCTAGGGT Pstutzeri_59 TGGAAAGTTCTCTGCATGTCAAGGC Vcholerae_59 CTAGCTTGTCAGTTTCAAATGCGAT Bcereus_59 GCAAGCTCTTAATCCATTCGCTCGA Pstutzeri_60 GGAAAGTTCTCTGCATGTCAAGGCC Vcholerae_60 TCTAGCTTGTCAGTTTCAAATGCGA Bcereus_60 AGCAAGCTCTTAATCCATTCGCTCG eukaryotes_1 AACTAAGAACGGCCATGCACCACCA sphingo_1_1 CCAGCTTGCTGCCCTCTGTACCATC alpha_7_1 ACATCTCTGTTTCCGCGACCGGGAT eukaryotes_2 CACCAACTAAGAACGGCCATGCACC sphingo_1_2 CAGCTTGCTGCCCTCTGTACCATCC alpha_7_2 CATCTCTGTTTCCGCGACCGGGATG eukaryotes_3 CCAACTAAGAACGGCCATGCACCAC sphingo_1_3 GCCAGCTTGCTGCCCTCTGTACCAT alpha_7_3 AAACATCTCTGTTTCCGCGACCGGG eukaryotes_4 ACCAACTAAGAACGGCCATGCACCA sphingo_1_4 TGCCAGCTTGCTGCCCTCTGTACCA alpha_7_4 GAAACATCTCTGTTTCCGCGACCGG eukaryotes_5 CCACCAACTAAGAACGGCCATGCAC sphingo_1_5 CAGTTTACGACCCAGAGGGCTGTCT alpha_7_5 AGAAACATCTCTGTTTCCGCGACCG eukaryotes_6 TCCACCAACTAAGAACGGCCATGCA sphingo_1_6 AGCAGTTTACGACCCAGAGGGCTGT alpha_7_6 AACATCTCTGTTTCCGCGACCGGGA eukaryotes_7 CAACTAAGAACGGCCATGCACCACC sphingo_1_7 AAGCAGTTTACGACCCAGAGGGCTG alpha_7_7 ATCTCTGTTTCCGCGACCGGGATGT eukaryotes_8 CTCCACCAACTAAGAACGGCCATGC sphingo_1_8 GCAGTTTACGACCCAGAGGGCTGTC alpha_7_8 CTGCCACTGTCCACCCGAGCAAGCT eukaryotes_9 TTGGAGCTGGAATTACCGCGGCTGC sphingo_1_9 CCGCCTACCTCTAGTGTATTCAAGC alpha_7_9 CCACTGTCCACCCGAGCAAGCTCGG eukaryotes_10 TCAGGCTCCCTCTCCGGAATCGAAC sphingo_1_10 CATTCCGCCTACCTCTAGTGTATTC alpha_7_10 GCCACTGTCCACCCGAGCAAGCTCG eukaryotes_11 TCTCAGGCTCCCTCTCCGGAATCGA sphingo_1_11 TGCTGTTGCCAGCTTGCTGCCCTCT alpha_7_11 AAACCTCTAGGTAGATACCCACGCG eukaryotes_12 TATTGGAGCTGGAATTACCGCGGCT sphingo_1_12 GCTGTTGCCAGCTTGCTGCCCTCTG alpha_7_12 CCAAACCTCTAGGTAGATACCCACG eukaryotes_13 ATTGGAGCTGGAATTACCGCGGCTG sphingo_1_13 TTGCTGTTGCCAGCTTGCTGCCCTC alpha_7_13 GTCTGCCACTGTCCACCCGAGCAAG eukaryotes_14 TAAGAACGGCCATGCACCACCACCC sphingo_1_14 CACATTCCGCCTACCTCTAGTGTAT alpha_7_14 CCACCCGAGCAAGCTCGGGTTTCTC eukaryotes_15 CTAAGAACGGCCATGCACCACCACC sphingo_1_15 GTCACATTCCGCCTACCTCTAGTGT alpha_7_15 TGCCACTGTCCACCCGAGCAAGCTC eukaryotes_16 ACTAAGAACGGCCATGCACCACCAC sphingo_1_16 TCACATTCCGCCTACCTCTAGTGTA alpha_7_16 CAAACCTCTAGGTAGATACCCACGC eukaryotes_17 CTCAGGCTCCCTCTCCGGAATCGAA sphingo_1_17 GCTTTCGCTTAGCCGCTAACTGTGT alpha_7_17 TCTGCCACTGTCCACCCGAGCAAGC eukaryotes_18 CTATTGGAGCTGGAATTACCGCGGC sphingo_1_18 CGCTTTCGCTTAGCCGCTAACTGTG alpha_7_18 CGTCTGCCACTGTCCACCCGAGCAA eukaryotes_19 AAGAACGGCCATGCACCACCACCCA sphingo_1_19 TCGCTTAGCCGCTAACTGTGTATCG alpha_7_19 TCCGAACCTCTAGGTAGATTCCCAC eukaryotes_20 AGGCTCCCTCTCCGGAATCGAACCC sphingo_1_20 TTCGCTTAGCCGCTAACTGTGTATC alpha_7_20 CACCCGAGCAAGCTCGGGTTTCTCG eukaryotes_21 CAGGCTCCCTCTCCGGAATCGAACC sphingo_1_21 CTTTCGCTTAGCCGCTAACTGTGTA alpha_7_21 ACCCGAGCAAGCTCGGGTTTCTCGT eukaryotes_22 GCTATTGGAGCTGGAATTACCGCGG sphingo_1_22 CTGTTGCCAGCTTGCTGCCCTCTGT alpha_7_22 CCGTCTGCCACTGTCCACCCGAGCA eukaryotes_23 TTTCTCAGGCTCCCTCTCCGGAATC sphingo1_23 GTTGCCAGCTTGCTGCCCTCTGTAC alpha_7_23 CCGAACCTCTAGGTAGATTCCCACG eukaryotes_24 GGCTCCCTCTCCGGAATCGAACCCT sphingo_1_24 TGTTGCCAGCTTGCTGCCCTCTGTA alpha_7_24 AACCTCTAGGTAGATACCCACGCGT eukaryotes_25 CACTCCACCAACTAAGAACGGCCAT sphingo_1_25 CGCTTAGCCGCTAACTGTGTATCGC alpha_7_25 TCCACCCGAGCAAGCTCGGGTTTCT archaea_1 TTGTGGTGCTCCCCCGCCAATTCCT sphingo_2_1 TCACCGCTACACCCCTCGTTCCGCT alpha_8_1 CTGCCACTGTCCACCCGAGCAAGCT archaea_2 TGCTCCCCCGCCAATTCCTTTAAGT sphingo_2_2 GCTATCGGCGTTCTGAGGAATATCT alpha_8_2 GCCACTGTCCACCCGAGCAAGCTCG archaea_3 CGCGCCTGCTGCGCCCCGTAGGGCC sphingo_2_3 CGCTATCGGCGTTCTGAGGAATATC alpha_8_3 AAACCTCTAGGTAGATACCCACGCG archaea_4 TTTCGCGCCTGCTGCGCCCCGTAGG sphingo_2_4 TCGGCGTTCTGAGGAATATCTATGC alpha_8_4 GTCTGCCACTGTCCACCCGAGCAAG archaea_5 TCGCGCCTGCTGCGCCCCGTAGGGC sphingo_2_5 TTCACCGCTACACCCCTCGTTCCGC alpha_8_5 CCACCCGAGCAAGCTCGGGTTTCTC archaea_6 TTCGCGCCTGCTGCGCCCCGTAGGG sphingo_2_6 TTTCACCGCTACACCCCTCGTTCCG alpha_8_6 TGCCACTGTCCACCCGAGCAAGCTC archaea_7 GTGCTCCCCCGCCAATTCCTTTAAG sphingo_2_7 TCGCTTTCGCTTAGCCACTTACTGT alpha_8_7 CAAACCTCTAGGTAGATACCCACGC archaea_8 GCTCCCCCGCCAATTCCTTTAAGTT sphingo_2_8 CGGCGTTCTGAGGAATATCTATGCA alpha_8_8 TCTGCCACTGTCCACCCGAGCAAGC archaea_9 GCGCCTGCTGCGCCCCGTAGGGCCT sphingo_2_9 AACTAATGGGGCGCATGCCCATCCC alpha_8_9 ACTGTCCACCCGAGCAAGCTCGGGT archaea_10 CGCCTGCTGCGCCCCGTAGGGCCTG sphingo_2_10 CGCTTAGCCACTTACTGTATATCGC alpha_8_10 CCACTGTCCACCCGAGCAAGCTCGG archaea_11 GCCTGCTGCGCCCCGTAGGGCCTGG sphingo_2_11 ACTAATGGGGCGCATGCCCATCCCG alpha_8_11 CCAAACCTCTAGGTAGATACCCACG archaea_12 GTTTCGCGCCTGCTGCGCCCCGTAG sphingo_2_12 GCCATGCAGCACCTCGTATAGAGTC alpha_8_12 GTCCACCCGAGCAAGCTCGGGTTTC archaea_13 CTTGTGGTGCTCCCCCGCCAATTCC sphingo_2_13 AGCCATGCAGCACCTCGTATAGAGT alpha_8_13 TCCACCCGAGCAAGCTCGGGTTTCT archaea_14 GGTTTCGCGCCTGCTGCGCCCCGTA sphingo_2_14 CAGCCATGCAGCACCTCGTATAGAG alpha_8_14 CGTCTGCCACTGTCCACCCGAGCAA archaea_15 AGGTTTCGCGCCTGCTGCGCCCCGT sphingo_2_15 ACAGCCATGCAGCACCTCGTATAGA alpha_8_15 TGTCCACCCGAGCAAGCTCGGGTTT archaea_16 CCTGCTGCGCCCCGTAGGGCCTGGA sphingo_2_16 CTTACTTGTCAGCCTACGCACCCTT alpha_8_16 ACCTCTAGGTAGATACCCACGCGTT archaea_17 CCTTGTGGTGCTCCCCCGCCAATTC sphingo_2_17 ACTTACTTGTCAGCCTACGCACCCT alpha_8_17 CACCCGAGCAAGCTCGGGTTTCTCG archaea_18 CCCCTTGTGGTGCTCCCCCGCCAAT sphingo_2_18 CCACTGACTTACTTGTCAGCCTACG alpha_8_18 TAAGCCGTCTGCCACTGTCCACCCG archaea_19 ACCCCTTGTGGTGCTCCCCCGCCAA sphingo_2_19 CACTGACTTACTTGTCAGCCTACGC alpha_8_19 ACCCGAGCAAGCTCGGGTTTCTCGT archaea_20 CCCTTGTGGTGCTCCCCCGCCAATT sphingo_2_20 GACTTACTTGTCAGCCTACGCACCC alpha_8_20 CCGTCTGCCACTGTCCACCCGAGCA archaea_21 CACCCCTTGTGGTGCTCCCCCGCCA  sphingo_2_21 TGACTTACTTGTCAGCCTACGCACC alpha_8_21 AACCTCTAGGTAGATACCCACGCGT archaea_22 GTGTGTGCAAGGAGCAGGGACGTAT sphingo_2_22 CTGACTTACTTGTCAGCCTACGCAC alpha_8_22 GCCGTCTGCCACTGTCCACCCGAGC archaea_23 TGTGTGCAAGGAGCAGGGACGTATT sphingo_2_23 ACTGACTTACTTGTCAGCCTACGCA alpha_8_23 TAGATACCCACGCGTTACTAAGCCG archaea_24 CGGTGTGTGCAAGGAGCAGGGACGT sphingo_2_24 CCATGCAGCACCTCGTATAGAGTCC alpha_8_24 AAGCCGTCTGCCACTGTCCACCCGA archaea_25 GGTGTGTGCAAGGAGCAGGGACGTA sphingo_2_25 CGCTTTCGCTTAGCCACTTACTGTA alpha_8_25 GTAGATACCCACGCGTTACTAAGCC bacteria_1 CGCTCGTTGCGGGACTTAACCCAAC sphingo_3_1 AGTTTCCTCGAGCTATGCCCCAGTT alpha_9_1 TCTCCGGCGACCAAACTCCCCATGT bacteria_2 GCTCGTTGCGGGACTTAACCCAACA sphingo_3_2 CGAGTTTCCTCGAGCTATGCCCCAG alpha_9_2 CGTCTCCGGCGACCAAACTCCCCAT bacteria_3 GACTTAACCCAACATCTCACGACAC sphingo_3_3 GTTTCCTCGAGCTATGCCCCAGTTA alpha_9_3 GTCTCCGGCGACCAAACTCCCCATG bacteria_4 AACCCAACATCTCACGACACGAGCT sphingo_3_4 TTTCCTCGAGCTATGCCCCAGTTAA alpha_9_4 CTCCGGCGACCAAACTCCCCATGTC bacteria_5 ACTTAACCCAACATCTCACGACACG sphingo_3_5 GAGTTTCCTCGAGCTATGCCCCAGT alpha_9_5 GCCGTCTCCGGCGACCAAACTCCCC bacteria_6 TAACCCAACATCTCACGACACGAGC sphingo_3_6 TCGAGTTTCCTCGAGCTATGCCCCA alpha_9_6 TCCGGCGACCAAACTCCCCATGTCA bacteria_7 GGACTTAACCCAACATCTCACGACA sphingo_3_7 TTACCGAAGTAAATGCTGCCCCTCG alpha_9_7 CCGTCTCCGGCGACCAAACTCCCCA bacteria_8 CTTAACCCAACATCTCACGACACGA sphingo_3_8 GTTGCTAGCTCTACCCTAAACAGCG alpha_9_8 CGCCGTCTCCGGCGACCAAACTCCC bacteria_9 TTAACCCAACATCTCACGACACGAG sphingo_3_9 AGTTGCTAGCTCTACCCTAAACAGC alpha_9_9 CCGGCGACCAAACTCCCCATGTCAA bacteria_10 GGGACTTAACCCAACATCTCACGAC sphingo_3_10 CCATTTACCGAAGTAAATGCTGCCC alpha_9_10 ACGCCGTCTCCGGCGACCAAACTCC bacteria_11 ACTGCTGCCTCCCGTAGGAGTCTGG sphingo_3_11 CATTTACCGAAGTAAATGCTGCCCC alpha_9_11 GAACTGAAGGACGCCGTCTCCGGCG bacteria_12 CTCGTTGCGGGACTTAACCCAACAT sphingo_3_12 CGCCATTTACCGAAGTAAATGCTGC alpha_9_12 CGGCGACCAAACTCCCCATGTCAAG bacteria_13 CGGGACTTAACCCAACATCTCACGA sphingo_3_13 TTGCTAGCTCTACCCTAAACAGCGC alpha_9_13 GTCGGCAGCCTCCCTTACGGGTCGG bacteria_14 TCGTTGCGGGACTTAACCCAACATC sphingo_3_14 GCCATTTACCGAAGTAAATGCTGCC alpha_9_14 GGTCGGCAGCCTCCCTTACGGGTCG bacteria_15 CGTTGCGGGACTTAACCCAACATCT sphingo_3_15 TCCTCGAGCTATGCCCCAGTTAAAG alpha_9_15 TGGTCGGCAGCCTCCCTTACGGGTC bacteria_16 GTTGCGGGACTTAACCCAACATCTC sphingo_3_16 TTCCTCGAGCTATGCCCCAGTTAAA alpha_9_16 TCGGCAGCCTCCCTTACGGGTCGGC bacteria_17 TGCGGGACTTAACCCAACATCTCAC sphingo_3_17 CAGTTGCTAGCTCTACCCTAAACAG alpha_9_17 GTGGTCGGCAGCCTCCCTTACGGGT bacteria_18 TTGCGGGACTTAACCCAACATCTCA sphingo_3_18 TGCTAGCTCTACCCTAAACAGCGCC alpha_9_18 CGTGGTCGGCAGCCTCCCTTACGGG bacteria_19 CCCCACTGCTGCCTCCCGTAGGAGT sphingo_3_19 CCGTCAGATCCTCTCGCAAGAGTAT alpha_9_19 CGGCAGCCTCCCTTACGGGTCGGCG bacteria_20 GCGGGACTTAACCCAACATCTCACG sphingo_3_20 CTCGAGCTATGCCCCAGTTAAAGGT alpha_9_20 CGCACCTCAGCGTCAGATCCGGACC bacteria_21 GCGCTCGTTGCGGGACTTAACCCAA sphingo_3_21 CCTCGAGCTATGCCCCAGTTAAAGG alpha_9_21 AATCTTTCCCCCTCAGGGCTTATCC bacteria_22 TCCCCACTGCTGCCTCCCGTAGGAG sphingo_3_22 CCAGTTGCTAGCTCTACCCTAAACA alpha_9_22 CGAACTGAAGGACGCCGTCTCCGGC bacteria_23 ATTCCCCACTGCTGCCTCCCGTAGG sphingo_3_23 TCTCTCTGGATGTCACTCGCATTCT alpha_9_23 TACCCTCTTCCGATCTCTAGCCTAG bacteria_24 TTCCCCACTGCTGCCTCCCGTAGGA sphingo_3_24 ATCTCTCTGGATGTCACTCGCATTC alpha_9_24 GGCAGCCTCCCTTACGGGTCGGCGA bacteria_25 ACCCAACATCTCACGACACGAGCTG sphingo_3_25 CTCTCTGGATGTCACTCGCATTCTA alpha_9_25 GGCGACCAAACTCCCCATGTCAAGG rhodobacter_1 TCCCCAGGCGGAATGCTTAATCCGT caldithrix_1_1 ACTCCTCAGAGCTTCATCGCCCACG alpha_10_1 CGCACCTGAGCGTCAGATCTAGTCC rhodobacter_2 CTCCCCAGGCGGAATGCTTAATCCG caldithrix_1_2 CTCCTCAGAGCTTCATCGCCCACGC alpha_10_2 TCGCACCTGAGCGTCAGATCTAGTC rhodobacter_3 ACTCCCCAGGCGGAATGCTTAATCC caldithrix_1_3 AACAGGGCTTTACACTCCTCAGAGC alpha_10_3 CGTGCGCCACTCTCCAGTTCCCGAA rhodobacter_4 CCCCAGGCGGAATGCTTAATCCGTT caldithrix_1_4 CACTCCTCAGAGCTTCATCGCCCAC alpha_10_4 CCGTGCGCCACTCTCCAGTTCCCGA rhodobacter_5 CACCGCGTCATGCTGTTACGCGATT caldithrix_1_5 ACAGGGCTTTACACTCCTCAGAGCT alpha_10_5 CCCGTGCGCCACTCTCCAGTTCCCG rhodobacter_6 TCACCGCGTCATGCTGTTACGCGAT caldithrix_1_6 ACACTCCTCAGAGCTTCATCGCCCA alpha_10_6 CTGAGCGTCAGATCTAGTCCAGGTG rhodobacter_7 ATTCACCGCGTCATGCTGTTACGCG caldithrix_1_7 CAGGGCTTTACACTCCTCAGAGCTT alpha_10_7 TTCGCACCTGAGCGTCAGATCTAGT rhodobacter_8 TAGCCCAACCCGTAAGGGCCATGAG caldithrix_1_8 TCCTCAGAGCTTCATCGCCCACGCG alpha_10_8 CCAACCGTTATCCCCCACTAAGAGG rhodobacter_9 TACTCCCCAGGCGGAATGCTTAATC caldithrix_1_9 TACACTCCTCAGAGCTTCATCGCCC alpha_10_9 TCCAACCGTTATCCCCCACTAAGAG rhodobacter_10 AGCCCAACCCGTAAGGGCCATGAGG caldithrix_1_10 CTTCTGGCACTCCCGACTTTCATGG alpha_10_10 GCACCTGAGCGTCAGATCTAGTCCA rhodobacter_11 GCCCAACCCGTAAGGGCCATGAGGA caldithrix_1_11 TTACACTCCTCAGAGCTTCATCGCC alpha_10_11 CCTGAGCGTCAGATCTAGTCCAGGT rhodobacter_12 AACGTATTCACCGCGTCATGCTGTT caldithrix_1_12 CCTCAGAGCTTCATCGCCCACGCGG alpha_10_12 GTTAGCCCACCGTCTTCGGGTAAAA rhodobacter_13 TTCACCGCGTCATGCTGTTACGCGA caldithrix_1_13 CCTAACAGGGCTTTACACTCCTCAG alpha_10_13 CCACTAAGAGGTAGGTCCCCACGCG rhodobacter_14 ACCGCGTCATGCTGTTACGCGATTA caldithrix_1_14 AGGGCTTTACACTCCTCAGAGCTTC alpha_10_14 TGAGCGTCAGATCTAGTCCAGGTGG rhodobacter_15 GCGGAATGCTTAATCCGTTAGGTGT caldithrix_1_15 TTCTGGCACTCCCGACTTTCATGGC alpha_10_15 ATCCCCCACTAAGAGGTAGGTCCCC rhodobacter_16 CCAACCCGTAAGGGCCATGAGGACT caldithrix_1_16 TCTGGCACTCCCGACTTTCATGGCG alpha_10_16 GCTTTCACCCCTGACTGGCAAGACC rhodobacter_17 CCCAGGCGGAATGCTTAATCCGTTA caldithrix_1_17 CTCAGAGCTTCATCGCCCACGCGGC alpha_10_17 CAACCGTTATCCCCCACTAAGAGGT rhodobacter_18 CCCAACCCGTAAGGGCCATGAGGAC caldithrix_1_18 GGGCTTTACACTCCTCAGAGCTTCA alpha_10_18 GCGTCACCGAAATCGAAATCCCGAC rhodobacter_19 AATTCCACTCACCTCTCTCGAACTC caldithrix_1_19 CTCCTAACAGGGCTTTACACTCCTC alpha_10_19 TGCGTCACCGAAATCGAAATCCCGA rhodobacter_20 GAATTCCACTCACCTCTCTCGAACT caldithrix_1_20 CTGGCACTCCCGACTTTCATGGCGT alpha_10_20 CGTCACCGAAATCGAAATCCCGACA rhodobacter_21 TATTCACCGCGTCATGCTGTTACGC caldithrix_1_21 TCAGAGCTTCATCGCCCACGCGGCG alpha_l0_21 CTGCGTCACCGAAATCGAAATCCCG rhodobacter_22 ACGTATTCACCGCGTCATGCTGTTA caldithrix_1_22 ACCTCTACAGCAGTCCCGAAGGAAG alpha_10_22 TTTCGCACCTGAGCGTCAGATCTAG rhodobacter_23 GAACGTATTCACCGCGTCATGCTGT caldithrix_1_23 CCCTCCTAACAGGGTTTTACACTCC alpha_10_23 CTTTCACCCCTGACTGGCAAGACCG rhodobacter_24 GGAATTCCACTCACCTCTCTCGAAC caldithrix_1_24 GGTCGAAACCTCCAACACCTAGTGC alpha_10_24 CTAAAAGGTTAGCCCACCGTCTTCG rhodobacter_25 GTAGCCCAACCCGTAAGGGCCATGA caldithrix_1_25 GTCGAAACCTCCAACACCTAGTGCC alpha_10_25 CCCACTAAGAGGTAGGTCCCCACGC margrpA_1 ACGAAGTTAGCCGGTGCTTTCTTGT chloroflexi_1_1 TCTCCGAGGAGTCGTTCCAGTTTCC alpha_12_1 CCGTGCGCCACTCTATAAATAGCGT margrpA_2 CACGAAGTTAGCCGGTGCTTTCTTG chloroflexi_1_2 CTCCGAGGAGTCGTTCCAGTTTCCC alpha_12_2 CCCGTGCGCCACTCTATAAATAGCG margrpA_3 GTTACTCACCCGTTCGCCAGTTTAC chloroflexi_1_3 ACGAATGGGTTTGACACCACCCACA alpha_12_3 CCAACCGTTATCCCGCAGAAAAAGG margrpA_4 TAAGGGACATACTGACTTGACATCA chloroflexi_1_4 CGAATGGGTTTGACACCACCCACAC alpha_12_4 CCCGCAGAAAAAGGCAGGTTCCCAC margrpA_5 ATAAGGGACATACTGACTTGACATC chloroflexi_1_5 CTCTCCGAGGAGTCGTTCCAGTTTC alpha_12_5 ACCGTTATCCCGCAGAAAAAGGCAG margrpA_6 AAGGGACATACTGACTTGACATCAT chloroflexi_1_6 TCCGAGGAGTCGTTCCAGTTTCCCT alpha_12_6 CAACCGTTATCCCGCAGAAAAACGC margrpA_7 TTACTCACCCGTTCGCCAGTTTACT chloroflexi_1_7 GAATGGGTTTGACACCACCCACACC alpha_12_7 CGTTCCAAACCGTTATCCCGCAGAA margrpA_8 CGTTACTCACCCGTTCGCCAGTTTA chloroflexi_1_8 GCTCTCCGAGGAGTCGTTCCAGTTT alpha_12_8 CCGCAGAAAAAGGCAGGTTCCCACG margrpA_9 GCGTTACTCACCCGTTCGCCAGTTT chloroflexi_1_9 CCGAGGAGTCGTTCCAGTTTCCCTT alpha_12_9 CGCAGAAAAAGGCAGGTTCCCACGC margrpA_10 CGCGTTACTCACCCGTTCGCCAGTT chloroflexi_1_10 CGCTCTCCGAGGAGTCGTTCCAGTT alpha_12_10 CCGTTATCCCGCAGAAAAAGGCAGG margrpA_11 ACATACTGACTTGACATCATCCCCA chloroflexi_1_11 AATGGGTTTGACACCACCCACACCT alpha_12_11 CGTTATCCCGCAGAAAAAGGCAGGT margrpA_12 TACTGACTTGACATCATCCCCACCT chloroflexi_1_12 CGAGGAGTCGTTCCAGTTTCCCTTC alpha_12_12 ACCCGTGCGCCACTCTATAAATAGC margrpA_13 GGACATACTGACTTGACATCATCCC chloroflexi_1_13 AGGAGTCGTTCCAGTTTCCCTTCAC alpha_12_13 CACCCGTGCGCCACTCTATAAATAG margrpA_14 GACATACTGACTTGACATCATCCCC chloroflexi_1_14 GAGGAGTCGTTCCAGTTTCCCTTCA alpha_12_14 TCCCGCAGAAAAAGGCAGGTTCCCA margrpA_15 ATACTGACTTGACATCATCCCCACC chloroflexi_1_15 CGCTTTGCGACATGAGCGTCAGGTT alpha_12_15 GCAGAAAAAGGCAGGTTCCCACGCG margrpA_16 CATACTGACTTGACATCATCCCCAC chloroflexi_1_16 TGAGCGTCAGGTTCAATGCCAGGGT alpha_12_16 GGAAACCAAACTCCCCATGTCAAGG margrpA_17 AGGGACATACTGACTTGACATCATC chloroflexi_1_17 ACGCTTTGCGACATGAGCGTCAGGT alpha_12_17 CCTCCTGCAAGCAGGTTAGCTCACC margrpA_18 GGGACATACTGACTTGACATCATCC chloroflexi_1_18 TCCCCACGCTTTGCGACATGAGCGT alpha_12_18 TTTCGCGCCTCAGCGTCAAAATCGG margrpA_19 ACGCGTTACTCACCCGTTCGCCAGT chloroflexi_1_19 TCAGGTTCAATGCCAGGGTACCGCT alpha_12_19 TTCGCGCCTCAGCGTCAAAATCGGA margrpA_20 GCACGAAGTTAGCCGGTGCTTTCTT chloroflexi_1_20 ATCATCTCGGCCTTCACGTTCGACT alpha_12_20 ACTCCCCATGTCAAGGACTGGTAAG margrpA_21 GGCACGAAGTTAGCCGGTGCTTTCT chloroflexi_1_21 TGCGACATGAGCGTCAGGTTCAATG alpha_12_21 GCCTCCTGCAAGCAGGTTAGCTCAC margrpA_22 TGGCACGAAGTTAGCCGGTGCTTTC chloroflexi_1_22 ATGAGCGTCAGGTTCAATGCCAGGG alpha_12_22 CAGAAAAAGGCAGGTTCCCACGCGT margrpA_23 ACTGACITGACATCATCCCCACCTT chloroflexi_1_23 CACGCTTTGCGACATGAGCGTCAGG alpha_12_23 TCCGGCGGACCTTTCCCCCGTAGGG margrpA_24 CTGGCACGAAGTTAGCCGGTGCTTT chloroflexi_1_24 CATGAGCGTCAGGTTCAATGCCAGG alpha_12_24 CCCCTCTTTCTCCGGCGGACCTTTC margrpA_25 ACGATTACTAGCGATTCCTGCTTCA chloroflexi_1_25 GTAATCATCTCGGCCTTCACGTTCG alpha_12_25 CCCCTCTTTCTCCGGCGGACCTTTC vibrionaceae_1 TATCCCCCACATCAGGGCAATTTCC chloroflexi_2_1 GGTGACTCCCCTTTCAGGTTGCTAC alpha_13_1 TCTAACTGTTCAAGCAGCCTGCGAG vibrionaceae_2 CGACATTACTCGCTGGCAAACAAGG chloroflexi_2_2 AGGTGACTCCCCTTTCAGGTTGCTA alpha_13_2 CTAACTGTTCAAGCAGCCTGCGAGC vibrionaceae_3 CCGACATTACTCGCTGGCAAACAAG chloroflexi_2_3 CCCTCCCCATTAAGCGGGGAGATTT alpha_13_3 TAACTGTTCAAGCAGCCTGCGAGCC vibrionaceae_4 CCCCACATCAGGGCAATTTCCTAGG chloroflexi_2_4 GCAAGCTTGGCTCATCGGTACCGTT alpha_13_4 GTCTAACTGTTCAAGCAGCCTGCGA vibrionaceae_5 CCCCTACATCAGGGCAATTTCCTAG chloroflexi_2_5 CTCTCCCGATGTTCCAAGCAAGCTT alpha_13_5 CGCTCCTCAGCGTCAGAAAATAGCC vibrionaceae_6 CCCACATCAGGGCAATTTCCTAGGC chloroflexi_2_6 CCCCTCCCCATTAAGCGGGGAGATT alpha_13_6 GCTCCTCAGCGTCAGAAAATAGCCA vibrionaceae_7 CCACATCAGGGCAATTTCCTAGGCA chloroflexi_2_7 TTCCAAGCAAGCTTGGCTCATCGGT alpha_13_7 TCGCTCCTCAGCGTCAGAAAATAGC vibrionaceae_8 TCCCCCACATCAGGGCAATTTCCTA chloroflexi_2_8 AGCAAGCTTGGCTCATCGGTACCGT alpha_13_8 CGTCTAACTGTTCAAGCAGCCTGCG vibrionaceae_9 CCCGACATTACTCGCTGGCAAACAA chloroflexi_2_9 ACTCTCCCGATGTTCCAAGCAAGCT alpha_13_9 AACTGTTCAAGCAGCCTGCGAGCCC vibrionaceae_10 ATCCCCCACATCAGGGCAATTTCCT chloroflexi_2_10 ACCCCTCCCCATTAAGCGGGGAGAT alpha_13_10 CACGTCGAACTGTTCAAGCAGCCTG vibrionaceae_11 TGGTTATCCCCCACATCAGGGCAAT chloroflexi_2_11 TCTCCCGATGTTCCAAGCAAGCTTG alpha_13_11 ACGTCTAACTGTTCAAGCAGCCTGC vibrionaceae_12 CCCCCACATCAGGGCAATTTCCCAG chloroflexi_2_12 CTCCCGATGTTCCAAGCAAGCTTGG alpha_13_12 ACTGTTCAAGCAGCCTGCGAGCCCT vibrionaceae_13 TCCCCCACATCAGGGCAATTTCCCA chloroflexi_2_13 AATGACCCCTCCCCATTAAGCGGGG alpha_13_13 CCGGGGATTTCACGTCTAAGTCTTC vibrionaceae_14 CCCCACATCAGGGCAATTTCCCAGG chloroflexi_2_14 GAATGACCCCTCCCCATTAAGCGGG alpha_13_14 CTCCTCAGCGTCAGAAAATAGCCAG vibrionaceae_15 CCCACATCAGGGCAATTTCCCAGGC chloroflexi_2_15 GTTCCAAGCAAGCTTGGCTCATCGG alpha_13_15 TTCAAGCAGCCTGCGAGCCCTTTAC vibrionaceae_16 CACATCAGGGCAATTTCCCAGGCAT chloroflexi_2_16 CGAATGACCCCTCCCCATTAAGCGG alpha_13_16 TGTTCAAGCAGCCTGCGAGCCCTTT vibrionaceae_17 CCACATCAGGGCAATTTCCCAGGCA chloroflexi_2_17 TGTTCCAAGCAAGCTTGGCTCATCG alpha_13_17 CTGTTCAAGCAGCCTGCGAGCCCTT vibrionaceae_18 ATCCCCCACATCAGGGCAATTTCCC chloroflexi_2_18 TCGAATGACCCCTCCCCATTAAGCG alpha_13_18 GTTCAAGCAGCCTGCGAGCCCTTTA vibrionaceae_19 TCCCGACATTACTCGCTGGCAAACA chloroflexi_2_19 AAGCAAGCTTGGCTCATCGGTACCG alpha_13_19 CGGCATTGCTGGATCAGAGTTGCCT vibrionaceae_20 GGTTATCCCCCACATCAGGGCAATT chloroflexi_2_20 TGACCCCTCCCCATTAAGCGGGGAG alpha_13_20 GGCATTGCTGGATCAGAGTTGCCTC vibrionaceae_21 CGCAAGTTGGCCGCCCTCTGTATGC chloroflexi_2_21 CCACTCTCCCGATGTTCCAAGCAAG alpha_13_21 CGCGGCATTGCTGGATCAGAGTTGC vibrionaceae_22 GCAAGTTGGCCGCCCTCTGTATGCG chloroflexi_2_22 CCTCCCCATTAAGCGGGGAGATTTC alpha_13_22 GCATTGCTGGATCAGAGTTGCCTCC vibrionaceae_23 ATGGTTATCCCCCACATCAGGGCAA chloroflexi_2_23 CAAGCTTGGCTCATCGGTACCGTTC alpha_13_23 GCGGCATTGCTGGATCAGAGTTGCC vibrionaceae_24 ACTCGCTGGCAAACAAGGATAAGGG chloroflexi_2_24 CCGATGTTCCAAGCAAGCTTGGCTC alpha_13_24 CCCGGGGATTTCACGTCTAACTGTT vibrionaceae_25 CGCATCTGAGTGTCAGTATCTGTCC chloroflexi_2_25 CACTCTCCCGATGTTCCAAGCAAGC alpha_13_25 ACGCGGCATTGCTGGATCAGAGTTG alteromonadales_1 CCCACTTGGGCCAATCTAAAGGCGA chlorella_p1_1 CGCCACTCATCGCAATCTGGCAAGC delta_1_1 CCGAACTACGAACTGCTTTCTGGGA alteromonadales_2 ATCCCACTTGGGCCAATCTAAAGGC chlorella_p1_2 GCCACTCATCGCAATGTGGCAAGCC delta_1_2 TCCGAACTACGAACTGCTTTCTGGG altermaonadales_3 TCCCACTTGGGCCAATCTAAAGGCG chlorella_p1_3 CCACTCATCGCAATCTGGCAAGCCA delta_1_3 TTGCTGCGGCACAGCAGGGGTCAAT altermaonadales_4 CCACTTGGGCCAATCTAAAGGCGAG chlorella_p1_4 CACTCATCGCAATCTGGCAAGCCAA delta_1_4 GTTTGCTGCGGCACAGCAGGGGTCA altermaonadales_5 CACTTGGGCCAATCTAAAGGCGAGA chlorella_p1_5 GCAAGCCAAATTGCATGAGTACGAC delta_1_5 TTTGCTGCGGCACAGCAGGGGTCAA altermaonadales_6 ACTTGGGCCAATCTAAAGGCGAGAG chlorella_p1_6 GCCAAATTGCATGCGTACGACTTGC delta_1_6 TTGCCCAACGACTTCTGGTACAACC alteromonadales_7 CTTGGGCCAATCTAAAGGCGAGAGC chlorella_p1_7 TGGCAAGCCAAATTGCATGCGTACG delta_1_7 GGTTTGCCCAACGACTTCTGGTACA alteromonadales_8 CACCTCAAGGCATGTTCCCAAGCAT chlorella_p1_8 CTGTGTCCACTCTGGAACTTCCCCT delta_1_8 TCCCCGAAGGGTTTGCCCAACGACT alteromonadales_9 TGAGCGTCAGTGTTGACCCAGGTGG chlorella_p1_9 CCGTCCGCCACTCATCGCAATCTGG delta_1_9 CCCCGAAGGGTTTGCCCAACGACTT alteromonadales_10 CGAAGCCCCCTTTGGTCCGTAGACA chlorella_p1_10 CCGCCACTCATCGCAATCTGGCAAG delta_1_10 CCGAAGGGTTTGCCCAACGACTTCT alteromonadales_11 ACAGAACCGAGGTTCCGAGCTTCTA chlorella_p1_11 CGTCCGCCACTCATCGCAATCTGGC delta_1_11 CCCGAAGGGTTTGCCCAACGACTTC alteromonadales_12 CAGAACCGAGGTTCCGAGCTTCTAG chlorella_p1_12 CCTGTGTCCACTCTGGAACTTCCCC delta_1_12 CCCGGGCTTTCACACCTGACTTAAA alteromonadales_13 AGAACCGAGGTTCCGAGCTTCTAGT chlorella_p1_13 GTCCGCCACTCATCGCAATCTGGCA delta_1_13 GCTTCCTTCAGTGGTACCGTCAACA alteromonadales_14 GAAAAACAGAACCGAGGTTCCGAGC chlorella_p1_14 TCCGCCACTCATCGCAATCTGGCAA delta_1_14 AGGCGCCTGCATCCCCGAAGGGTTT alteromonadales_15 GAACCGAGGTTCCGAGCTTCTAGTA chlorella_p1_15 ACCTGTGTCCACTCTGGAACTTCCC delta_1_15 GGCGCCTGCATCCCCGAAGGGTTTG alteromonadales_16 CCGAGGTTCCGAGCTTCTAGTAGAC chlorella_p1_16 GGCAAGCCAAATTGCATGCGTACGA delta_1_16 GCGCCTGCATCCCCGAAGGGTTTGC alteromonadales_17 CGAGGTTCCGAGCTTCTAGTAGACA chlorella_p1_17 CTGGCAAGCCAAATTGCATGCGTAC delta_1_17 GCATCCCCGAAGGGTTTGCCCAACG alteromonadales_18 AACCGAGGTTCCGAGCTTCTAGTAG chlorella_p1_18 CCCGTCCGCCACTCATCGCAATCTG delta_1_18 ATCCCCGAAGGGTTTGCCCAACGAC alteromonadales_19 ACCGAGGTTCCGAGCTTCTAGTAGA chlorella_p1_19 CACCTGTGCCACTCTGGAACTTTCC delta_1_19 CATCCCCGAAGGGTTTGCCCAACGA alteromonadales_20 AACAGAACCGAGGTTCCGAGCTTCT chlorella_p1_20 ACCCGTCCGCCACTCATCGCAATCT delta_1_20 ACCTTAGGCGCCTGCATCCCCGAAG alteromonadales_21 AAACAGAACCGAGGTTCCGAGCTTC chlorella_p1_21 CCACCTGTGTCCACTCTGGAACTTC delta_1_21 CCTTAGGCGCCTGCATCCCCGAAGG alteromonadales_22 CCGAAGCCCCCTTTGGTCCGTAGAC chlorella_p1_22 CACCCGTCCGCCACTCATCGCAATC delta_1_22 TACCTTAGGCGCCTGCATCCCCGAA alteromonadales_23 GAAGCCCCCTTTGGTCCGTAGACAT chlorella_p1_23 TCACCCGTCCGCCACTCATCGCAAT delta_1_23 ATACCTTAGGCGCCTGCATCCCCGA alteromonadales_24 AAGCCCCCTTTGGTCCGTAGACATT chlorella_p1_24 ACCACCTGTGTCCACTCTGGAACTT delta_1_24 CTTAGGCGCCTGCATCCCCGAAGGG alteromonadales_25 CCACCTCAAGGCATGTTCCCAAGCA chlorella_p1_25 CACCACCTGTGTCCACTCTGGAACT delta_1_25 CATACCTTAGGCGCCTGCATCCCCG polaribacters_1 GCCAGATGGCTGCTCATTGTCCATA plastid_1_1 GGTCTCACGACTTGGCATCTCATTG delta_2_1 CTCCAGTCTTTCGATAGGATTCCCG polaribacters_2 TGCCAGATGGCTGCTCATTGTCCAT plastid_1_2 TCTCCCTAGGCAGGTTTTTGACCTG delta_2_2 GGCCACCCTTGATCCAAAAACCCGA polaribaclers_3 TTGCCAGATGGCTGCTCATTGTCCA plastid_1_3 CCACGTGGATTCGATACACGCAATG delta_2_3 AGGCCACCCTTGATCCAAAAACCCG polaribacters_4 CCAGATGGCTGCTCATTGTCCATAC plastid_1_4 ATGCACCACCTGTATGTGTCTGCCG delta_2_4 AAGGGCACTCCAGTCTTTCGATAGG polaribacters_5 GTTGCCAGATGGCTGCTCATTGTCC plastid_1_5 CACCACCTGTATGTGTCTGCCGAAG delta_2_5 GAGGCCACCCTTGATCCAAAAACCC polaribacters_6 TCCCTCAGCGTCAGTACATACGTAG plastid_1_6 AACACCACGTGGATTCGATACACGC delta_2_6 GAAGGGCACTCCAGTCTTTCGATAG polaribacters_7 CCCTCAGCGTCAGTACATACGTAGT plastid_1_7 ACCACCTGTATGTGTCTGCCGAAGC delta_2_7 ACCCTAGCAAGCTAGAGTGTTCTCG polaribacters_8 GTCCCTCAGCGTCAGTACATACGTA plastid_1_8 CTTCTCCCTAGGCAGGTTTTTGACC delta_2_8 AGAGGCCACCCTTGATCCAAAAACC polaribacters_9 CAGATGGCTGCTCATTGTCCATACC plastid_1_9 TGCACCACCTGTATGTGTCTGCCGA delta_2_9 AGAGGCCACCCTTGATCCAAAAACC polaribacters_10 TTCGCATAGTGGCTGCTCATTGTCC plastid_1_10 ACACCACGTGGATTCGATACACGCA delta_2_10 ACATGTAGAGGCCACCCTTGATCCA polaribacters_11 CGTCCCTCAGCGTCAGTACATACGT plastid_1_11 CCACCTGTATGTGTCTGCCGAAGCA delta_2_11 TACATGTAGAGGCCACCCTTGATCC polaribacters_12 AGACCCCCTACCTATCGTTGCCATG plastid_1_12 GCACCACCTGTATGTGTCTGCCGAA delta_2_12 CCCCGAAGGGCACTCCAGTCTTTCG polaribacters_13 CGCTTAGTCACTGAGCTAATGCCCA plastid_1_13 CACCACGTGGATTCGATACACGCAA delta_2_13 CCCTAGCAAGCTAGAGTGTTCTCGT polaribacters_14 TGTTGCCAGATGGCTGCTCATTGTC plastid_1_14 CTCACGACTTGGCATCTCATTGTCC delta_2_14 GCTTACATGTAGAGGCCACCCTTGA polaribacters_15 GATTCGCTCCTATTCGCATAGTGGC plastid_1_15 CAGGTACACGTCAGAAACTTCCTTC delta_2_15 GGGCACTCCAGTCTTTCGATAGGAT polaribacters_16 TCGTCCCTCAGCGTCAGTACATACG plastid_1_16 CTCCCTAGGCAGGTTTTTGACCTGT delta_2_16 CCGAAGGGCACTCCAGTCTTTCGAT polaribacters_17 TCGCTTAGTCACTGAGCTAATGCCC plastid_1_17 CGGTCTCACGACTTGGCATCTCATT delta_2_17 CGAAGGGCACTCCAGTCTTTCGATA polaribacters_18 TCGCATAGTGGCTGCTCATTGTCCA plastid_1_18 GACCAACTACTGATCGTCACCTTGG delta_2_18 AGGGCACTCCAGTCTTTCGATAGGA polaribacters_19 CAGACCCCCTACCTATCGTTGCCAT plastid_1_19 GCTTCTCCCTAGGCAGGTTTTTGAC delta_2_19 CCCGAAGGGCACTCCAGTCTTTCGA polaribacters_20 TTCGTCCCTCAGCGTCAGTACATAC plastid_1_20 CACCTGTATGTGTCTGCCGAAGCAC delta_2_20 CCAGTCTTTCGATAGGATTCCCGGG polaribacters_21 CTCTCTGTTGCCAGATGGCTGCTCA plastid_1_21 CTGTATGTGTCTGCCGAAGCACTTC delta_2_21 TCCAGTCTTTCGATAGGATTCCCGG polaribacters_22 GCAGATTCTATACGCGTTACGCACC plastid_1_22 CATGCACCACCTGTATGTGTCTGCC delta_2_22 GTCTTTCGATAGGATTCCCGGGATG polaribacters_23 GGCAGATTCTATACGCGTTACGCAC plastid_1_23 AGGTACACGTCAGAACTTCCTCCC delta_2_23 CTTTCGATAGGATTCCCGGGATGTC polaribacters_24 CACCTCTGACTTAATTGACCGCCTG plastid_1_24 TCGGTCTCACGACTTGGCATCTCAT delta_2_24 CAGTCTTTCGATAGGATTCCCGGGA polaribacters_25 CCTCTGACTTAATTGACCGCCTGCG plastid_1_25 CCTTCTACTTCGACTCTACTCGAGC delta_2_25 GGGCTCCCCGAAGGGCACTCCAGTC desulfovibrionales_1 CCCGAGCATGCTGATCTCGAATTAC plastid_2_1 CAGGTAACGTCAGAACTTCCTCCCT delta_31 GGCACAGAAAGGGTCAACACTTCCT desulfovibrionales_2 CACCCGAGCATGCTGATCTCGAATT plastid_2_2 AGGTAACGTCAGAACTTCCTCCCTG delta_3_2 TCGGCACAGAAAGGGTCAACACTTC desulfovibrionales_3 TCACCCGAGCATGCTGATCTCGAAT plastid_2_3 GGTAACGTCAGAACTTCCTCCCTGA delta_3_3 CGGCACAGAAAGGGTCAACACTTCC desuIfovibrionales_4 TTCACCCGAGCATGCTGATCTCGAA plastid_2_4 TCAGGTAACGTCAGAACTTCCTCCC delta_3_4 CTTCGGCACAGAAAGGGTCAACACT desulfovibrionales_5 GCACCCTCTAATTTCCTAGAGGTCC plastid_2_5 CGCGTTAGCTATAATACCGCATGGG delta_3_5 CACTTTACTCTCCCGACGAATCGGA desulfovibrionales_6 AGGGCACCCTCTAATTTCCTAGAGG plastid_2_6 AATACCGCATGGGTCGATACATGCG delta_3_6 CCACTTTACTCTCCCGACGAATCGG desulfovibrionales_7 GGGCACCCTCTAATTTCCTAGAGGT plastid_2_7 CTGTATGTACGTTCCCGAAGGTGGT delta_3_7 GCTTCGGCACAGAAAGGGTCAACAC desulfovibrionales_8 CCCTCTAATTTCCTAGAGGTCCCCT plastid_2_8 CCTGTATGTACGTTCCCGAAGGTGG delta_3_8 CTCTCCCGACGAATCGGAATTTCTC desulfovibrionales_9 ACCCTCTAATTTCCTAGAGGTCCCC plastid_2_9 TCAGCCGCGAGCTCCTCTCTAGGCA delta_3_9 CCGACGAATCGGAATTTCTCGTTCG desulfovibrionales_10 ATTTCCTAGAGGTCCCCTGGATGTC plastid_2_10 ATACCGCATGGGTCGATACATGCGA delta_3_10 GCCACTTTACTCTCCCGACGAATCG desulfovibrionales_11 AGGGTACCGTCAAATGCCTACCCTA plastid_2_11 ACCTGTATGTACGTTCCCGAAGGTG delta_3_11 AGCTTCGGCACAGAAAGGGTCAACA desulfovibrionales_12 GAGGGTACCGTCAAATGCCTACCCT plastid_2_12 GCCGCGAGCTCCTCTCTAGGCAGAA delta_3_12 ACTCTCACGAGTTCGCTACCCTTTG desulfovibrionales_13 GGGTACCGTCAAATGCCTACCCTAT plastid_2_13 GCGCCTTCCTCCAAACGGTTAGAAT delta_3_13 TCTCCCGACGAATCGGAATTTCTCG desulfovibrionales_14 TTTCCTAGAGGTCCCCTGGATGTCA plastid_2_14 AGCCGCGAGCTCCTCTCTAGGCAGA delta_3_14 TAGCTTCGGCACAGAAAGGGTCAAC desulfovibrionales_15 TTCCTAGAGGTCCCCTGGATGTCAA plastid_2_15 CAGCCGCGAGCTCCTCTCTAGGCAG delta_3_15 CTCTCACGAGTTCGCTACCCTTTGT desulfovibrionales_16 TGAGGGTACCGTCAAATGCCTACCC plastid_2_16 CACCTGTATGTACGTTCCCGAAGGT delta_3_16 GTGCTGGTTACACCCGAAGGCAATC desulfovibrionales_17 CTCTAATTTCCTAGAGGTCCCCTGG plastid_2_17 AATCAGCCGCGAGCTCCTCTCTAGG delta_3_17 CGCCACTTTACTCTCCCGACGAATC desulfovibrionales_18 CACCCTCTAATTTCCTAGAGGTCCC plastid_2_18 TAATCAGCCGCGAGCTCCTCTCTAG delta_3_18 CTCCCGACGAATCGGAATTTCTCGT desulfovibrionales_19 GGCACCCTCTAATTTCCTAGAGGTC plastid_2_19 ATCAGCCGCGAGCTCCTCTCTAGGC delta_3_19 CTTACTCTCACGAGTTCGCTACCCT desulfovibrionales_20 CCTCTAATTTCCTAGAGGTCCCCTG plastid_2_20 GGCGCCTTCCTCCAAACGGTTAGAA delta_3_20 TGTGCTGGTTACACCCGAAGGCAAT desulfovibrionales_21 CAACCGTTATCCCCGTCTTGAAGGT plastid_2_21 CCGCGAGCTCCTCTCTAGGCAGAAA delta_3_21 CTCACGAGTTCGCTACCCTTTGTAC desulfovibrionales_22 ATCAAAGGCTGTTCCACCGTTGAGC plastid_2_22 GCATGGGTCGATACATGCGACATCT delta_3_22 CTGTGCTGGTTACACCCGAAGGCAA desulfovibrionales_23 TTGCTCGTTAGCTCGCCGGCTTCGG plastid_2_23 CCGCATGGGTCGATACATGCGACAT delta_3_23 TCGCCACTTTACTCTCCCGACGAAT desulfovibrionales_24 ATTGCTCGTTAGCTCGCCGGCTTCG plastid_2_24 TACCGCATGGGTCGATACATGCGAC delta_3_24 CCTGTGCTGGTTACACCCGAAGGCA desulfovibrionales_25 CCTAGAGGTCCCCTGGATGTCAAGC plastid_2_25 ACCGCATGGGTCGATACATGCGACA delta_3_25 GCTTACTCTCACGAGTTCGCTACCC aquaficae_1 AACCAGACGCTCCACCGGTTGTGCG plastid_3_1 CACCGTCGTATATCTGACCGACGAT altero_1_1 CCCACTTGGGCCAATCTAAAGGCGA aquaficae_2 ACCAGACGCTCCACCGGTTGTGCGG plastid_3_2 TTCACCGTCGTATATCTGACCGACG altero_1_2 ATCCCACTTGGGCCAATCTAAAGGC aquaficae_3 AAACCAGACGCTCCACCGGTTGTGC plastid_3_3 TCACCGTCGTATATCTGACCGACGA altero_1_3 TCCCACTTGGGCCAATCTAAAGGCG aquaficae_4 TGCCACTGTAGCGCCTGTGTAGCCC plastid_3_4 GTAGCCGAGTTTCAGGCTACAATCC altero_1_4 CCACTTGGGCCAATCTAAAGGCGAG aquaficae_5 TAAACCAGACGCTCCACCGGTTGTG plastid_3_5 TAGCCGAGTTTCAGGCTACAATCCG altero_1_5 CACTTGGGCCAATCTAAAGGCGAGA aquaficae_6 GCCACTGTAGCGCCTGTGTAGCCCA plastid_3_6 GACCTCATCCTCACCTTCCTCCAAT altero_1_6 ACTTGGGCCAATCTAAAGGCGAGAG aquaficae_7 CCAGACGCTCCACCGGTTGTGCGGG plastid_3_7 AGCCGAGTTTCAGGCTACAATCCGA altero_1_7 CTTGGGCCAATCTAAAGGCGAGAGC aquaficae_8 CCACTGTAGCGCCTGTGTAGCCCAG plastid_3_8 GCCGAGTTTCAGGCTACAATCCGAA altero_1_8 CTGTCAGTAACGTCACAGCTAGCAG aquaficae_9 GCATAAAGGGCATACTGACCTGACG plastid_3_9 CCGAGTTTCAGGCTACAATCCGAAC altero_1_9 ACAGAACCGAGGTTCCGAGCTTCTA aquaficae_10 TTAAACCAGACGCTCCACCGGTTGT plastid_3_10 CTCCCGTAGGAGTCTGTTCCGTTCT altero_1_10 CAGAACCGAGGTTCCGAGCTTCTAG aquaficae_11 CATTGCCCACGATTCCCCACTGCTG plastid_3_11 CCTCCCGTAGGAGTCTGTTCCGTTC altero_1_11 AGAACCGAGGTTCCGAGCTTCTAGT aquaficae_12 ATTGCCCACGATTCCCCACTGCTGC plastid_3_12 TCCCGTAGGAGTCTGTTCCGTTCTA altero_1_12 GAAAAACAGAACCGAGGTTCCGAGC aquaticae_13 CCATTGCCCACGATTCCCCACTGCT plastid_3_13 CCCGTAGGAGTCTGTTCCGTTCTAA altero_1_13 GAACCGAGGTTCCGAGCTTCTAGTA aquaficae_14 GCCCATTGCCCACGATTCCCCACTG plastid_3_14 TGACCTCATCCTCACCTTCCTCCAA altero_1_14 CCGAGGTTCCGAGCTTCTAGTAGAC aquaficae_15 CCCATTGCCCACGATTCCCCACTGC plastid_3_15 CTAAAGCATTCATCCTCCACGCGGT altero_1_15 CGAGGTTCCGAGCTTCTAGTAGACA aquaficae_16 CGCCCATTGCCCACGATTCCCCACT plastid_3_16 CCTAAAGCATTCATCCTCCACGCGG altero_1_16 AACCGAGGTTCCGAGCTTCTAGTAG aquaficae_17 TGCCCACGATTCCCCACTGCTGCCC plaslid_3_17 CCCTAAAGCATTCATCCTCCACGCG altero_1_17 ACCGAGGTTCCGAGCTTCTAGTAGA aquaficae_18 ATTAAACCAGACGCTCCACCGGTTG plastid_3_18 ACCCTAAAGCATTCATCCTCCACGC altero_1_18 AACAGAACCGAGGTTCCGAGCTTCT aquaficae_19 TTGCCCACGATTCCCCACTGCTGCC plastid_3_19 ACATAAGGGGCATGCTGACTTGACC altero_1_19 AAACAGAACCGAGGTTCCGAGCTTC aquaficae_20 GCCCACGATTCCCCACTGCTGCCCC plastid_3_20 GTTCCGTTCTAAATCCCAGTGTGGC altero_1_20 CCAACTGTTGTCCCCCACCTCAAGG aquaficae_21 CAGACGCTCCACCGGTTGTGCGGGC plastid_3_21 CATAAGGGGCATGCTGACTTGACCT altero_1_21 CCGGACTACGACGCACTTTAAGTGA aquaficae_22 GGCATAAAGGGCATACTGACCTGAC plastid_3_22 GCGGTATTGCTTGGTCAAGCTTTCG altero_1_22 TGGGCCAATCTAAAGGCGAGAGCCG aquaficae_23 GCAGTTCGGAATGCCTTGCCGAAGT plastid_3_23 CGGTATTGCTTGGTCAAGCTTTCGC altero_1_23 GGGCCAATCTAAAGGCGAGAGCCGA aquaficae_24 CAGTTCGGAATGCCTTGCCGAAGTT plastid_3_24 CACGCGGTATTGCTTGGTCAAGCTT altero_1_24 TTGGGCCAATCTAAAGGCGAGAGCC aquaficae_25 CGCAGTTCGGAATGCCTTGCCGAAG plastid_3_25 CATCCTCCACGCGGTATTGCTTGGT altero_1_25 GGTTCCGAGCTTCTAGTAGACATCG bacilli_1 CACTCTGCTCCCGAAGGAGAAGCCC plastid_4_1 CTTAAGCGCCGCCCTCCGAATGGTT altero_2_1 TCTCACTTGGGCCTCTCTTTGCGCC bacilli_2 GTCACTCTGCTCCCGAAGGAGAAGC plastid_4_2 CCTTAAGCGCCGCCCTCCGAATGGT altero_2_2 CCCCTCGCAAAGGCAAGTTCCCAAG bacilli_3 CTGCTCCCGAAGGAGAAGCCCTATC plastid_4_3 TACCTTAAGCGCCGCCCTCCGAATG altero_2_3 CCCTCGCAAAGGCAAGTTCCCAAGC bacilli_4 TCACTCTGCTCCCGAAGGAGAAGCC plastid_4_4 ACCTTAAGCGCCGCCCTCCGAATGG altero_2_4 TCACTTGGGCCTCTCTTTGCGCCGG bacilli_5 TCTGCTCCCGAAGGAGAAGCCCTAT plastid_4_5 AGCCCTACCTTAAGCGCCGCCCTCC altero_2_5 CTTGGGCCTCTCTTTGCGCCGGAGC bacilli_6 TGCTCCCGAAGGAGAAGCCCTATCT plastid_4_6 TTAAGCGCCGCCCTCCGAATGGTTA altero_2_6 CGACATTCTTTAAGGGGTCCGCTCC bacilli_7 CTCTGCTCCCGAAGGAGAAGCCCTA plastid_4_7 TAAGCGCCGCCCTCCGAATGGTTAG altero_2_7 CACTTGGGCCTCTCTTTGCGCCGGA bacilli_8 GCTCCCGAAGGAGAAGCCCTATCTC plastid_4_8 TAGCCCTACCTTAAGCGCCGCCCTC altero_2_8 CTCACTTGGGCCTCTCTTTGCGCCG bacilli_9 ACTCTGCTCCCGAAGGAGAAGCCCT plaslid_4_9 CTACCTTAAGCGCCGCCCTCCGAAT altero_2_9 ACTTGGGCCTCTCTTTGCGCCGGAG bacilli_10 CCGAAGCCGCCTTTCAATTTCGAAC plastid_4_10 GCCCTACCTTAAGCGCCGCCCTCCG altero_2_10 CTACGACATTCTTTAAGGGGTCCGC bacilli_11 CGTCCGCCGCTAACTTCATAAGAGC plastid_4_11 CCCTACCTTAAGCGCCGCCCTCCGA altero_2_11 CCGGACTACGACATTCTTTAAGGGG bacilli_12 GTCCGCCGCTAACTTCATAAGAGCA plastid_4_12 CCTACCTTAAGCGCCGCCCTCCGAA altero_2_12 ATCTCACTTGGGCCTCTCTTTGCGC bacilli_13 CCGCCGCTAACTTCATAAGAGCAAG plastid_4_13 CTAGCCCTACCTTAAGCGCCGCCCT altero_2_13 CCCCCTCGCAAAGGCAAGTTCCCAA bacilli_14 AGCCGAAGCCGCCTTTCAATTTCGA plastid_4_14 ACTAGCCCTACCTTAAGCGCCGCCC altero_2_14 ACATTCTTTAAGGGGTCCGCTCCAC bacilli_15 CTCCCGAAGGAGAAGCCCTATCTCT plastid_4_15 AAGCGCCGCCCTCCGAATGGTTAGG altero_2_15 TTGGGCCTCTCTTTGCGCCGGAGCC bacilli_16 CAGCCGAAGCCGCCTTTCAATTTCG plastid_4_16 CACTAGCCCTACCTTAAGCGCCGCC altero_2_16 TCCCCCTCGCAAAGGCAAGTTCCCA bacilli_17 CTGTCACTCTGCTCCCGAAGGAGAA plastid_4_17 CGCCGCCCTCCGAATGGTTAGGCTA altero_2_17 CCTCGCAAAGGCAAGTTCCCAAGCA bacilli_18 GCCGAAGCCGCCTTTCAATTTCGAA plastid_4_18 GCGCCGCCCTCCGAATGGTTAGGCT altero_2_18 GGGTCCGCTCCACATCACTGTCTCG bacilli_19 CCCGTCCGCCGCTAACTTCATAAGA plastid_4_19 GCCGCCCTCCGAATGGTTAGGCTAA altero_2_19 ACGACATTCTTTAAGGGGTCCGCTC bacilli_20 CCGTCCGCCGCTAACTTCATAAGAG plastid_4_20 AGCGCCGCCCTCCGAATGGTTAGGC altero_2_20 CATTCTTTAAGGGGTCCGCTCCACA bacilli_21 CGCCGCTAACTTCATAAGAGCAAGC plastid_4_21 ACGAGATTAGCTAGCCTTCGCAGGT altero_2_21 GACATTCTTTAAGGGGTCCGCTCCA bacilli_22 CCCGAAGGAGAAGCCCTATCTCTAG plastid_4_22 CCGCCCTCCGAATGGTTAGGCTAAC altero_2_22 AATCTCACTTGGGCCTCTCTTTGCG bacilli_23 CGAAGGAGAAGCCCTATCTCTAGGG plastid_4_23 CGCCCTCCGAATGGTTAGGCTAACG altero_2_23 TAAGGGGTCCGCTCCACATCACTGT bacilli_24 CCGAAGGAGAAGCCCTATCTCTAGG plastid_4_24 GCCCTCCGAATGGTTAGGCTAACGA altero_2_24 ATCCCCCTCGCAAAGGCAAGTTCCC bacilli_25 TGTCACTCTGCTCCCGAAGGAGAAG plastid_4_25 TCACTAGCCCTACCTTAAGCGCCGC altero_2_25 GGTCCGCTCCACATCACTGTCTCGC crenarch_1_1 AGCCTGTACGTTGAGCGTACAGATT plastid_5_1 CTCTACCCCTACCATACTCAAGCCT colwel_1_1 TGCGCCACTCACGGATCAAGTCCAC crenarch_1_2 CCTGTACGTTGAGCGTACAGATTTA plastid_5_2 GACGTCGTCCTCCAAATGGTTAGAC colwel_1_2 CTGCGCCACTCACGGATCAAGTCCA crenarch_1_3 GCCTGTACGTTGAGCGTACAGATTT plastid_5_3 CCTTAGCGTCGTCCTCCAAATGGT colwel_1_3 GCTGCGCCACTCACGGATCAAGTCC crenarch_1_4 GAGCGTACAGATTTAACCGAAAACT plastid_5_4 ACCTTAGACGTCGTCCTCCAAATGG colwel_1_4 TAGCTGCGCCACTCACGGATCAAGT crenarch_1_5 TGAGCGTACAGATTTAACCGAAAAC plastid_5_5 CCTCTACCCCTACCATACTCAAGCC colwel_1_5 GTTAGCTGCGCCACTCACGGATCAA crenarch_1_6 CAGCCTGTACGTTGAGCGTACAGAT plastid_5_6 GCTAGTTCTCGCGAATTTGCGACTC colwel_1_6 CGTTAGCTGCGCCACTCACGGATCA crenarch_1_7 CCTTGTCACGAACCTCAAGTTCGAT plastid_5_7 CCTCTCGGCATATGGGGATTTAGCT colwel_1_7 GTGCGTTAGCTGCGCCACTCACGGA crenarch_1_8 CTTGTCACGAACCTCAAGTTCGATA plastid_5_8 GACTAACGGTGTTGGGTATGACCAG colwel_1_8 TGCGTTAGCTGCGCCACTCACGGAT crenarch_1_9 TTGTCACGAACCTCAAGTTCGATAA plastid_5_9 ACTAACGGTGTTGGGTATGACCAGC colwel_1_9 TTAGCTGCGCCACTCACGGATCAAG crenarch_1_10 CTGTACGTTGAGCGTACAGATTTAA plastid_5_10 CCAACAGTTATTCCCCTCCTAAGGG colwel_1_10 GCGTTAGCTGCGCCACTCACGGATC crenarch_1_11 GTCACGAACCTCAAGTTCGATAACG plastid_5_11 CTCTCGGCATATGGGGATTTAGCTG colwel_1_11 AGCTGCGCCACTCACGGATCAAGTC crenarch_1_12 TTCCCTTGTCACGAACCTCAAGTTC plastid_5_12 GCGCGAGCTCATCCTTAGGCAGTGT colwel_1_12 GCGGTATTGCTGCCCTCTGTACCTG crenarch_1_13 TCACGAACCTCAAGTTCGATAACGC plastid_5_13 CGCGAGCTCATCCTTAGGCAGTGTA colwel_1_13 CGCGGTATTGCTGCCCTCTGTACCT crenarch_1_14 TGTCACGAACCTCAAGTTCGATAAC plasiid_5_14 GCGAGCTCATCCTTAGGCAGTGTAA colwel_1_14 GGATCAAGTCCACGAACGGCTAGTT crenarch_1_15 CTGCAGCACTGCATTGGCCACAAGC plastid_5_15 CACCTCTCGGCATATGGGGATTTAG colwel_1_15 CGGATCAAGTCCACGAACGGCTAGT crenarch_1_16 GCAGCCTGTACGTTGAGCGTACAGA plastid_5_16 ACCTCTCGGCATATGGGGATTTAGC colwel_1_16 GCGCCACTCACGGATCAAGTCCACG crenarch_1_17 CACGAACCTCAAGTTCGATAACGCC plastid_5_17 GCAGCCTACAATCCGAACTTGGACA colwel_1_17 ACGGATCAAGTCCACGAACGGCTAG crenarch_1_18 TGTACGTTGAGCGTACAGATTTAAC plasiid_5_18 GGCGCGAGCTCATCCTTAGGCAGTG colwel_1_18 CACGGATCAAGTCCACGAACGGCTA crenarch_1_19 CGTTGAGCGTACAGATTTAACCGAA plastid_5_19 CGGCAGTCTCTCTAGAGATCCCAAT colwel_1_19 CGCCACTCACGGATCAAGTCCACGA crenarch_1_20 GTACGTTGAGCGTACAGATTTAACC plastid_5_20 ATCACCGGCAGTCTCTCTAGAGATC colwel_1_20 GCCACTCACGGATCAAGTCCACGAA crenarch_1_21 CCTGCAGCACTGCATTGGCCACAAG plastid_5_21 CACCGGCAGTCTCTCTAGAGATCCC colwel_1_21 TCACGGATCAAGTCCACGAACGGCT crenarch_1_22 GGCAGCCTGTACGTTGAGCGTACAG plastid_5_22 ACCGGCAGTCTCTCTAGAGATCCCA colwel_1_22 GATCAAGTCCACGAACGGCTAGTTG crenarch_1_23 TACGTTGAGCGTACAGATTTAACCG plastid_5_23 CCGGCAGTCTCTCTAGAGATCCCAA colwel_1_23 ACTCACGGATCAAGTCCACGAACGG crenarch_1_24 ACGTTGAGCGTACAGATTTAACCGA plastid_5_24 TTCGCCTCTCAGTGTCAGTAATGGC colwel_1_24 CACTCACGGATCAAGTCCACGAACG crenarch_1_25 CCACTCCCTAGCTCTGCAGTATTCC plastid_5_25 TCGCCTCTCAGTGTCAGTAATGGCC colwel_1_25 CTCACGGATCAAGTCCACGAACGGC acido_1_1 TGCAGCACCTCTTCTGGAGTCCCCG margrpA_1_1 GCTCCGGTACCGAAGGGGTCGAATC altero_3_1 CAACTGTTGTCCCCCACGTTTTGGC acido_1_2 GCCGGCAGTCCCCCCAAAGTCCCCG margrpA_1_2 AGCTCCGGTACCGAAGGGGTCGAAT altero_3_2 AACTGTTGTCCCCCACGTTTTGGCA acido_1_3 CCATGCAGCACCTCTTCTGGAGTCC margrpA_1_3 CACCCGATTCGGGTACTACTGACTT altero_3_3 CCCCACGTTTTGGCATATTCCCAAG acido_1_4 CATGCAGCACCTCTTCTGGAGTCCC margrpA_1_4 ACCCGATTCGGGTACTACTGACTTC altero_3_4 CCCACGTTTTGGCATATTCCCAAGC acido_1_5 GCGCCGGCAGTCCCCCCAAAGTCCC margrpA_1_5 CTCCGGTACCGAAGGGGTCGAATCC altero_3_5 TCCCCCACGTTTTGGCATATTCCCA acido_1_6 ATGCAGCACCTCTTCTGGAGTCCCC margrpA_1_6 CCACCCGATTCGGGTACTACTGACT altero_3_6 CCCCCACGTTTTGGCATATTCCCAA acido_1_7 CGCCGGCAGTCCCCCCAAAGTCCCC margrpA_1_7 GCCACCCGATTCGGGTACTACTGAC altero_3_7 CCAACTGTTGTCCCCCACGTTTTGG acido_1_8 GCAGCACCTCTTCTGGAGTCCCCGA margrpA_1_8 GGCCACCCGATTCGGGTACTACTGA altero_3_8 GTCCCCCACGTTTTGGCATATTCCC acido_1_9 CAGCACCTCITCrGGAGTCCCCGAA margrpA_1_9 TAGCTCCGGTACCGAAGGGGTCGAA altero_3_9 ACTGTTGTCCCCCACGTTTTGGCAT acido_1_10 AGCACCTCTTCTGGAGTCCCCGAAG margrpA_1_10 TCCGGTACCGAAGGGGTCGAATCCC altero_3_10 TCCAACTGTTGTCCCCCACGTTTTG acido_1_11 CCGGCAGTCCCCCCAAAGTCCCCGG margrpA_1_11 GAAGGGGTCGAATCCCCCGACACCA altero_3_11 TGTCCCCCACGTTTTGGCATATTC acido_1_12 GCAGTCCCCCCAAAGTCCCCGGCAT margrpA_1_12 AAGGGGTCGAATCCCCCGACACCAA altero_312 GCATACCATCGCTGGTTAGCAACCC acido_1_13 GCACCTCTTCTGGAGTCCCCGAAGG margrpA_1_13 CTTCCCTTACGACAGACCTTTACGC altero_313 CGCATACCATCGCTGGTTAGCAACC acido_1_14 GCCATGCAGCACCTCTTCTGGAGTC margrpA_1_14 CCCGATTCGGGTACTACTGACTTCC altero_314 TCGCATACCATCGCTGGTTAGCAAC acido_1_15 ACCTCTTCTGGAGTCCCCGAAGGGA margrpA_1_15 ACAACTGTATCCCGAAGGATCCGCT altero_315 CTGTTGTCCCCCACGTTTTGGCATA acido_1_16 CACCTCTTCTGGAGTCCCCGAAGGG margrpA_1_16 CAACTGTATCCCGAAGGATCCGCTG altero_316 CTTGGGCTAATCAAAACGCGCAAGG acido_1_17 CGGCAGTCCCCCCAAAGTCCCCGGC margrpA_1_17 AACTGTATCCCGAAGGATCCGCTGC altero_317 TCCCACTTGGGCTAATCAAAACGCG acido_1_18 CCCCGAAGGGGCCTTACCGCTCAAC margrpA_1_18 AACAACTGTATCCCGAAGGATCCGC altero_3_18 TTGGGCTAATCAAAACGCGCAAGGC acido_1_19 CCTCTTCTGGAGTCCCCGAAGGGAA margrpA_1_19 GTTAGCTCCGGTACCGAAGGGGTCG altero_3_19 CCCACTTGGGCTAATCAAAACGCGC acido_1_20 GGCAGTCCCCCCAAAGTCCCCGGCA margrpA_1_20 TTAGCTCCGGTACCGAAGGGGTCGA altero_3_20 TCACCGGCAGTCTCCCTATAGTTCC acido_1_21 AGCCATGCAGCACCTCTTCTGGAGT margrpA_1_21 GCGTTAGCTCCGGTACCGAAGGGGT altero_3_21 TGGGCTAATCAAAACGCGCAAGGCC acido_1_22 CAGCCATGCAGCACCTCTTCTGGAG margrpA_1_22 CGTTAGCTCCGGTACCGAAGGGGTC altero_3_22 CCACTTGGGCTAATCAAAACGCGCA acido_1_23 CCCCCGAAGGGGCCTCACCGCTCAA margrpA_1_23 TGCGTTAGCTCCGGTACCGAAGGGG altero_3_23 ATAGTTCCCGACATAACTCGCTGGC acido_1_24 ACAGCCATGCAGCACCTCTTCTGGA margrpA_1_24 TCCCTTACGACAGACCTTTACGCTC altero_3_24 CCATCGCTGGTTAGCAACCCTTTGT acido_1_25 CCGAAGGGGCCTTACCGCTCAACTT margrpA_1_25 ACTGTATCCCGAAGGATCCGCTGCA altero_3_25 GGGCTAATCAAAACGCGCAAGGCCC acido_2_1 GTCAACTCCCTCCACACCAAGTGTT margrpA_2_1 GCTGCCTTCGCATTTGACTTTCCTC gamma_1_1 CTAAAAGGTCAAGCCTCCCAACGGC acido_2_2 GGTCAACTCCCTCCACACCAAGTGT margrpA_2_2 GGCTGCCTTCGCATTTGACTTTCCT gamma_1_2 ACTAAAAGGTCAAGCCTCCCAACGG acido_2_3 GGGTCAACTCCCTCCACACCAAGTG margrpA_2_3 AGGCTGCCTTCGCATTTGACTTTCC gamma_1_3 GAAGAGGCCCTCTTTCCCTCTTAAG acido_2_4 TCAACTCCCTCCACACCAAGTGTTC margrpA_2_4 ACAACTGTGCTCCGAAGAGCCCGCT gamma_1_4 CACTAAAAGGTCAAGCCTCCCAACG acido_2_5 GGGGTCACCTCCCTCCACACCAAGT margrpA_2_5 TAACAACTGTGCTCCGAAGAGCCCG gamma_1_5 GCATGTATTAGGCCTGCCGCCAACG acido_2_6 AGGGGTCAACTCCCTCCACACCAAG margrpA_2_6 AACAACTGTGCTCCGAAGAGCCCGC gamma_1_6 GGCTCCTCCAATAGTGAGAGCTTTC acido_2_7 CAACTCCCTCCACACCAAGTGTTCA margrpA_2_7 GATACCATCTTCGGGTACTGCAGAC gamma_1_7 AAGAGGCCCTCTTTCCCTCTTAAGG acido_2_8 AAGGGGTCAACTCCCTCCACACCAA margrpA_2_8 TTAACAACTGTGCTCCGAAGAGCCC gamma_1_8 CAAGAAGAGGCCCTCTTTCCCTCTT acido_2_9 GAAGGGGTCAACTCCCTCCACACCA margrpA_2_9 CAACTGTGCTCCGAAGAGCCCGCTG gamma_1_9 TCAAGAAGAGGCCCTCTTTCCCTCT acido_2_10 AACTCCCTCCACACCAAGTGTTCAT margrpA_2_10 CAGAAGGCTGCCTTCGCATTTGACT gamma_1_10 TAGCTGCGCCACTAAAAGGTCAAGC acido_2_11 ACTCCCTCCACACCAAGTGTTCATC margrpA_2_11 ACCATCTTCGGGTACTGCAGACTTC gamma_1_11 CAGGCTCCTCCAATAGTGAGAGCTT acido_2_12 CTCCCTCCACACCAAGTGTTCATCG margrpA_2_12 TTGCGGTTAGGATACCATCTTCGGG gamma_1_12 CTCAGCGTCAGTATCAATCCAGGGG acido_2_13 CAGTCCCCGTAGAGTTCCCGCCATG margrpA_2_13 CTTGCGGTTAGGATACCATCTTCGG gamma_1_13 AAAGGTCAAGCCTCCCAACGGCTAG acido_2_14 TCCCCGTAGAGTTCCCGCCATGACG margrpA_2_14 CCTTGCGGTTAGGATACCATCTTCG gamma_1_14 AGAGGCCCTCTTTCCCTCTTAAGGC acido_2_15 GTCCCCGTAGAGTTCCCGCCATGAC margrpA_2_15 CCATCTTCGGGTACTGCAGACTTCC gamma_1_15 GAGGCCCTCTTTCCCTCTTAAGGCG acido_2_16 AGTCCCCGTAGAGTTCCCGCCATGA margrpA_2_16 GGATACCATCTTCGGGTACTGCAGA gamma_1_16 AGAGGCCCTCTTTCCCTCTTAAGGC acido_2_17 GCAGTCCCCGTAGAGTTCCCGCCAT margrpA_2_17 ACCTGCCTTACCTTAAACAGCTCCC gamma_1_17 CCCCCTCTATCGTACTCTAGCCTAT acido_2_18 GGCAGTCCCCGTAGAGTTCCCGCCA margrpA_2_18 CCTGCCTTACCTTAAACAGCTCCCT gamma_1_18 CCCCTCTATCGTACTCTAGCCTATC acido_2_19 CCGGCACGGAAGGGGTCAACTCCCT margrpA_2_19 CCAGAAGGCTGCCTTCGCATTTGAC gamma_1_19 TTCAAGAAGAGGCCCTCTTTCCCTC acido_2_20 ACGCGCTGGCAACTACGGGTAAGGG margrpA_2_20 TGCGGTTAGGATACCATCTTCGGGT gamma_1_20 AGGCCCTCTTTCCCTCTTAAGGCGT acido_2_21 GACGCGCTGGCAACTACGGGTAAGG margrpA_2_21 CGAAGAGCCCGCTGCATTATTTGGT gamma_1_21 GCCCTCTTTCCCTCTTAAGGCGTAT acido_2_22 TGACGCGCTGGCAACTACGGGTAAG margrpA_2_22 CCACCATGAATTCTGCGTTCCTCTC gamma_1_22 CCCTCTTTCCCTCTTAAGGCGTATG acido_2_23 AGCTCCGGCACGGAAGGGGTCAACT margrpA_2_23 CCTCCTTGCGGTTAGGATACCATCT gamma_1_23 CTCTTTCCCTCTTAAGGCGTATGCG acido_2_24 GCTCCGGCACGGAAGGGGTCAACTC margrpA_2_24 CATCTTCGGGTACTGCAGACTTCCA gamma_1_24 CCTCTTTCCCTCTTAAGGCGTATGC acido_2_25 CTCCGGCACGGAAGGGGTCAACTCC margrpA_2_25 CGGTTAGGATACCATCTTCGGGTAC gamma_1_25 GGCCCTCTTTCCCTCTTAAGGCGTA acido_3_1 CTCACGGCATTCGTCCCACTCGACA OP10_1_1 CCGCTTGCACGGGCAGTTCCGTAAG gamma_2_1 TACCTGCTAGCAACCAGGGATAGGG acido_3_2 CGAGGTCCCCACGGTGTCATGCGGT OP10_1_2 CCCGCTTGCACGGGCAGTTCCGTAA gamma_2_2 CAGCATTACCTGCTAGCAACCAGGG acido_3_3 TCACCCTCACGGCATTCGTCCCACT OP10_1_3 CGCTTGCACGGGCAGTTCCGTAAGA gamma_2_3 TTACCTGCTAGCAACCAGGGATAGG acido_3_4 AGGTCCCCACGGTGTCATGCGGTAT OP10_1_4 TCCCGCTTGCACGGGCAGTTCCGTA gamma_2_4 ACCTGCTAGCAACCAGGGATAGGGG acido_3_5 GGACCGAGGTCCCCACGGTGTCATG OP10_1_5 GGGTGCAGACAATTCAGGTGACTTG gamma_2_5 TCAGCATTACCTGCTAGCAACCAGG acido_3_6 CCGAGGTCCCCACGGTGTCATGCGG OP10_1_6 CTCCCGCTTGCACGGGCAGTTCCGT gamma_2_6 TCTCCCTGGAGTTCTCAGCATTACC acido_3_7 ACCCTCACGGCATTCGTCCCACTCG OP10_1_7 CCTCCCGCTTGCACGGGCAGTTCCG gamma_2_7 GTCTCCCTGGAGTTCTCAGCATTAC acido_3_8 ACCGAGGTCCCCACGGTGTCATGCG OP10_1_8 GCTTGCACGGGCAGTTCCGTAAGAG gamma_2_8 CAGTCTCCCTGGAGTTCTCAGCATT acido_3_9 CACCCTCACGGCATTCGTCCCACTC OP10_1_9 CGGGTGCAGACAATTCAGGTGACTT gamma_2_9 TCCCTGGAGTTCTCAGCATTACCTG acido_3_10 GACCGAGGTCCCCACGGTGTCATGC OP10_1_10 CCGTAAGAGTTCCCGACTTTACGCT gamma_2_10 CTCCCTGGAGTTCTCAGCATTACCT acido_3_11 CCTCACGGCATTCGTCCCACTCGAC OP10_1_11 GCAGACAATTCAGGTGACTTGACGG gamma_2_11 GCAGTCTCCCTGGAGTTCTCAGCAT acido_3_12 TTCACCCTCACGGCATTCGTCCCAC OP10_1_12 TCGGGTGCAGACAATTCAGGTGACT gamma_2_12 GGCAGTCTCCCTGGAGTTCTCAGCA acido_3_13 GAGGTCCCCACGGTGTCATGCGGTA OP10_1_13 CGTAAGAGTTCCCGACTTTACGCTG gamma_2_13 CCTGCTAGCAACCAGGGATAGGGGT acido_3_14 CCCTCACGGCATTCGTCCCACTCGA OP10_1_14 TTGCACGGGCAGTTCCGTAAGAGTT gamma_2_14 TGCTAGCAACCAGGGATAGGGGTTG acido_3_15 GGTCCCCACGGTGTCATGCGGTATT OP10_1_15 TCCGTAAGAGTTCCCGACTTTACGC gamma_2_15 CTGCTAGCAACCAGGGATAGGGGTT acido_3_16 GTCCCCACGGTGTCATGCGGTATTA OP10_1_16 GGCAGTTCCGTAAGAGTTCCCGACT gamma_2_16 TAGCAACCAGGGATAGGGGTTGCGC acido_3_17 GATTGTTCACCCTCACGGCATTCGT OP10_1_17 CTTGCACGGGCAGTTCCGTAAGACT gamma_2_17 AGCAACCAGGGATAGGGGTTGCGCT acido_3_18 AGGACCGAGGTCCCCACGGTGTCAT OP10_1_18 CGGGCAGTTCCGTAAGAGTTCCCGA gamma_2_18 CTCAGCATTACCTGCTAGCAACCAG acido_3_19 ATTGTTCACCTTCACGGCATTCGTC OP10_1_19 TGCACGGGCAGTTCCGTAAGAGTTC gamma_2_19 CTAGCAACCAGGGATAGGGGTTGCG acido_3_20 TTGTTCACCCTCACGGCATTCGTCC OP10_1_20 ACGGGCAGTTCCGTAAGAGTTCCCG gamma_2_20 GCTAGCAACCAGGGATAGGGGTTGC acido_3_21 TGTTCACCCTCACGGCATTCGTCCC OP10_1_21 GCACGGGCAGTTCCGTAAGAGTTCC gamma_2_21 GCATTACCTGCTAGCAACCAGGGAT acido_3_22 GGATTGTTCACCCTCACGGCATTCG OP10_1_22 CACGGGCAGTTCCGTAAGAGTTCCC gamma_2_22 AGCATTACCTGCTAGCAACCAGGGA acido_3_23 CACGGCATTCGTCCCACTCGACAGG OP10_1_23 GCAGTTCCGTAAGAGTTCCCGACTT gamma_2_23 TCGCGAGTTGGCAGCCCTCTGTACG acido_3_24 TCACGGCATTCGTCCCACTCGACAG OP10_1_24 GGGCAGTTCCGTAAGAGTTCCCGAC gamma_2_24 CTCGCGAGTTGGCAGCCCTCTGTAC acido_3_25 GCTTTGATCGCAAGGACCGAGGTCC OP10_1_25 CCCCCTTACTCCCCACACCTTAGAC gamma_2_25 CGCGAGTTGGCAGCCCTCTGTACGC actino_1_1 AAACCTAGATCCGTCATCCCACACG OP3_1_1 ATCCAAGGGTGATAGGTCCTTACGG gamma_3_1 TGCGACACCGAAGGGCAACCCCCCC actino_1_2 CAAACCTAGATCCGTCATCCCACAC OP3_1_2 TCCAAGGGTGATAGGTCCTTACGGA gamma_3_2 CTGCGACACCGAAGGGCAACCCCCC actino_1_3 CACCACCTGTATAGGGCGCTAATGC OP3_1_3 CCAAGGGTGATAGGTCCTTACGGAT gamma_3_3 GACTAGTTCCGAGTATGTCAAGGGC actino_1_4 ACCACCTGTATAGGGCGCTAATGCA OP3_1_4 TGTTCTCCCCTGCTGACAGGAGTTT gamma_3_4 GCTGCGACACCGAAGGGCAACCCCC actino_1_5 CCACCTGTATAGGGCGCTAATGCAC OP3_1_5 TTGTTCTCCCCTGCTGACAGGAGTT gamma_3_5 AACGCGCTAGCTGCGACACCGAAGG actino_1_6 CACCTGTATAGGGCGCTAATGCACA OP3_1_6 CTTGTTCTCCCCTGCTGACAGGAGT gamma_3_6 TAACGCGCTAGCTGCGACACCGAAG actino_1_7 GCACCACCTGTATAGGGCGCTAATG OP3_1_7 GTTCTCCCCTGCTGACAGGAGTTTA gamma_3_7 TTACTTAACCGCCAACGCGCGCTTT actino_1_8 AACCTAGATCCGTCATCCCACACGC OP3_1_8 CATCCAAGGGTGATAGGTCCTTACG gamma_3_8 ACGCGCTAGCTGCGACACCGAAGGG actino_1_9 TGCACCACCTGTATAGGGCGCTAAT OP3_1_9 TCGACAGGTTATCCCGAACCCTAGG gamma_3_9 TTAACGCGCTAGCTGCGACACCGAA actino_1_10 AGCCCTGAACTTTCACGACCGACTT OP3_1_10 TTCGACAGGTTATCCCGAACCCTAG gamma_3_10 CGCGCTAGCTGCGACACCGAAGGGC actino_1_11 GCCCTGAACTTTCACGACCGACTTG OP3_1_11 TTCTCCCCTGCTGACAGGAGTTTAC gamma_3_11 TACTTAACCGCCAACGCGCGCTTTA actino_1_12 GAGCCCTGAACTTTCACGACCGACT OP3_1_12 CCATCCAAGGGTGATAGGTCCTTAC gamma_3_12 AGCTGCGACACCGAAGGGCAACCCC actino_1_13 AGCGTCGATAGCGGCCCAGTGAGCT OP3_1_13 TGATAGGTCCTTACGGATCCCCATC gamma_3_13 CTTACTTAACCGCCAACGCGCGCTT actino_1_14 GCGTCGATAGCGGCCCAGTGAGCTG OP3_1_14 TCTCCCCTGCTGACAGGAGTTTACA gamma_3_14 ATCCGACTTACTTAACCGCCAACGC actino_1_15 CGTCGATAGCGGCCCAGTGAGCTGC OP3_1_15 CGGATCCCCATCTTTCCCTCATGTT gamma_3_15 CGACTTACTTAACCGCCAACGCGCG actino_1_16 CAGCGTCGATAGCGGCCCAGTGAGC OP3_1_16 TCCTTGCCGGTTAGGCAACCTACTT gamma_3_16 TCCGACTTACTTAACCGCCAACGCG actino_1_17 CCCTGAACTTTCACGACCGACTTGT OP3_1_17 AGTGCGCACCGACCGAAGTCGGTGT gamma_3_17 CTTAACGCGCTAGCTGCGACACCGA actino_1_18 TGAGCCCTGAACTTTCACGACCGAC OP3_1_18 CCAGTAATGCGCCTTCGCGACTGGT gamma_3_18 ACTTACTTAACCGCCAACGCGCGCT actino_1_19 ACCTAGATCCGTCATCCCACACGCG OP3_1_19 AGAGTGCGCACCGACCGAAGTCGGT gamma_3_19 GCGCTAGCTGCGACACCGAAGGGCA actino_1_20 CTCGGGCTATCCCAGTAACTAAGGT OP3_1_20 TCGAAAAGCACAGGACGTATCCGGT gamma_3_20 CCGACTTACTTAACCGCCAACGCGC actino_1_21 CCTCGGGCTATCCCAGTAACTAAGG OP3_1_21 CTGTGCTTCGAAAAGCACAGGACGT gamma_3_21 ACTTAACCGCCAACGCGCGCTTTAC actino_1_22 TCGATAGCGGCCCAGTGAGCTGCCT OP3_1_22 CCTTAGAGTGCGCACCGACCGAAGT gamma_3_22 CATCCGACTTACTTAACCGCCAACG actino_1_23 GTCGATAGCGGCCCAGTGAGCTGCC OP3_1_23 GCCCTCCTTGCCGGTTAGGCAACCT gamma_3_23 TCTTCACACACGCGGCATTGCTAGA actino_1_24 CGATAGCGGCCCAGTGAGCTGCCTT OP3_1_24 CTCCTTGCCGGTTAGGCAACCTACT gamma_3_24 AGAACTTAACGCGCTAGCTGCGACA actino_1_25 TCCTCGGGCTATCCCAGTAACTAAG OP3_1_25 CAGTAATGCGCCTTCGCGACTGGTG gamma_3_25 ACTTAACGCGCTAGCTGCGACACCG actino_2_1 CCGGTTTCCCCAAGTGCAAGCACTT OP9_1_1 GGGCAAGATAATGTCAAGTCCCGGT gamma_4_1 ACACCGAAAGGCAAACCCTCCCGAC actino_2_2 CAAGCACTTGGTTCGTCCCTCGACT OP9_1_2 GCTGGCACATAATTAGCCGGAGCTT gamma_4_2 GACACCGAAAGGCAAACCCTCCCGA actino_2_3 GCCGGTTTCCCCAAGTGCAAGCACT OP9_1_3 TGCTGGCACATAATTAGCCGGAGCT gamma_4_3 CACCGAAAGGCAAACCCTCCCGACA actino_2_4 GCTTCGACACGGAAATCGTGAACTG OP9_1_4 CCACTTACCAGGGTAGATTACCCAC gamma_4_4 ACCGAAAGGCAAACCCTCCCGACAT actino_2_5 TTCGCCGGTTTCCCCAAGTGCAAGC OP9_1_5 CCCCACTTACAGGGTAGATTACCCA gamma_4_5 CGACACCGAAAGGCAAACCCTCCCG actino_2_6 CGACACGGAAATCGTGAACTGATCC OP9_1_6 CCCCCACTTACAGGGTAGATTACCC gamma_4_6 CCGAAAGGCAAACCCTCCCGACATC actino_2_7 GACACGGAAATCGTGAACTGATCCC OP9_1_7 CTGCTAACCTCATCATCCCGAAGGA gamma_4_7 GCGACACCGAAAGGCAAACCCTCCC actino_2_8 ACACGGAAATCGTGAACTGATCCCC OP9_1_8 TCTGCTAACCTCATCATCCCGAAGG gamma_4_8 CGAAAGGCAAACCCTCCCGACATCT actino_2_9 CGCCGGTTTCCCCAAGTGCAAGCAC OP9_1_9 CTGCTGGCACATAATTAGCCGGAGC gamma_4_9 GCTGCGACACCGAAAGGCAAACCCT actino_2_10 ACGGAAATCGTGAACTGATCCCCAC OP9_1_10 CCACTTACAGGGTAGATTACCCACG gamma_4_10 AGCTGCGACACCGAAAGGCAAACCC actino_2_11 TCGCCGGTTTCCCCAAGTGCAAGCA OP9_1_11 GACGGGCAAGATAATGTCAAGTCCC gamma_4_11 TTGGCTAGCCATTGCTGGTTTGCAG actino_2_12 CACGGAAATCGTGAACTGATCCCCA OP9_1_12 TCCCCCACTTACAGGGTAGATTACC gamma_4_12 TGGCTAGCCATTGCTGGTTTGCAGC actino_2_13 CGGTTTCCCCAAGTGCAAGCACTTG OP9_1_13 GCAGTCTGCCTAGAGTGCACTTGTA gamma_4_13 GGATTGGCTAGCCATTGCTGGTTTG actino_2_14 AAGTGCAAGCACTTGGTTCGTCCCT OP9_1_14 GCTGCTGGCACATAATTAGCCGGAG gamma_4_14 GATTGGCTAGCCATTGCTGGTTTGC actino_2_15 GTTCGCCGGTTTCCCCAAGTGCAAG OP9_1_15 GGGTACCGTCAGGCTTAAGGGTTTA gamma_4_15 GGGATTGGCTAGCCATTGCTGGTTT actino_2_16 CGGAAATCGTGAACTGATCCCCACA OP9_1_16 CACTTACAGGGTAGATTACCCACGC gamma_4_16 GGCTAGCCATTGCTGGTTTGCAGCC actino_2_17 GCAAGCACTTGGTTCGTCCCTCGAC OP9_1_17 GGCAGTCTGCCTAGAGTGCACTTGT gamma_4_17 GAAAGGCAAACCCTCCCGACATCTA actino_2_18 CGTTCGCCGGTTTCCCCAAGTGCAA OP9_1_18 GGTTATCCCCCACTTACAGGGTAGA gamma_4_18 CTGCGACACCGAAAGGCAAACCCTC actino_2_19 AAGCACTTGGTTCGTCCCTCGACTT OP9_1_19 GAGGGTTATCCCCCACTTACAGGGT gamma_4_19 TGCGACACCGAAAGGCAAACCCTCC actino_2_20 GGTTTCCCCAAGTGCAAGCACTTGG OP9_1_20 GGGTTATCCCCCACTTACAGGGTAG gamma_4_20 AGGGATTGGCTAGCCATTGCTGGTT actino_2_21 AGTGCAAGCACTTGGTTCGTCCCTC OP9_1_21 GTCAGAGATAGACCAGAAAGCCGCC gamma_4_21 AAGGGATTGGCTAGCCATTGCTGGT actino_2_22 CAAGTGCAAGCACTTGGTTCGTCCC OP9_1_22 GGGGTACCGTCAGGCTTAAGGGTTT gamma_4_22 TAAGGGATTGGCTAGCCATTGCTGG actino_2_23 CCGTTCGCCGGTTTCCCCAAGTGCA OP9_1_23 AGGGTTATCCCCCACTTACAGGGTA gamma_4_23 TAGCTGCGACACCGAAAGGCAAACC actino_2_24 CCGTAGTTATCCCGGTGTACAGGGC OP9_1_24 CGGCAGTCTGCCTAGAGTGCACTTG gamma_4_24 TTAGCTGCGACACCGAAAGGCAAAC actino_2_25 CCTCAAGCCTTGCAGTATCGACTGC OP9_1_25 CTCCGCATTATCTGCGGCAGTCTGC gamma_4_25 GTTAGCTGCGACACCGAAAGGCAAA bacter_1_1 GTTTCCGCGACTGTCATTCCACGTT plancto_1_1 TGCAACACCTGTGCAGGTCACACCC gamma_5_1 CCACTAAGGGACAAATTCCCCCAAC bacter_1_2 TTCCGCGACTGTCATTCCACGTTCG plancto_1_2 GCAACACCTGTGCAGGTCACACCCG gamma_5_2 CGCCACTAAGGGACAAATTCCCCCA bacter_1_3 ACGTTTCCGCGACTGTCATTCCACG plancto_1_3 ATGCAACACCTGTGCAGGTCACACC gamma_5_3 GCCACTAAGGGACAAATTCCCCCAA bacter_1_4 TTTCCGCGACTGTCATTCCACGTTC plancto_1_4 AACACCTGTGCAGGTCACACCCGAA gamma_5_4 CACTAAGGGACAAATTCCCCCAACG bacter_1_5 CACGTTTCCGCGACTGTCATTCCAC plancto_1_5 CAACACCTGTGCAGGTCACACCCGA gamma_5_5 ACTAAGGGACAAATTCCCCCAACGG bacter_1_6 TCACGTTTCCGCGACTGTCATTCCA plancto_1_6 TGTGCAGGTCACACCCGAAGGTAAT gamma_5_6 CTAAGGGACAAATTCCCCCAACGGC bacter_1_7 CGTTTCCGCGACTGTCATTCCACGT plancto_1_7 GTGCAGGTCACACCCGAAGGTAATC gamma_5_7 GCGCCACTAAGGGACAAATTCCCCC bacter_1_8 TGTCATTCCACGTTCGAGCCCAGGT plancto_1_8 TGCAGGTCACACCCGAAGGTAATCA gamma_5_8 GGTACCGTCAAGACGCGCATGGATT bacter_1_9 CTGTCATTCCACGTTCGAGCCCAGG plancto_1_9 CTGTGCAGGTCACACCCGAAGGTAA gamma_5_9 AGGTACCGTCAAGACGCGCAGTTAT bacter_1_10 CCGCGACTGTCATTCCACGTTCGAG plancto_1_10 CCTGTGCAGGTCACACCCGAAGGTA gamma_5_10 TAGGTACCGTCAAGACGCGCAGTTA bacter_1_11 ACTGTCATTCCACGTTCGAGCCCAG plancto_1_11 ACACCTGTGCAGGTCACACCCGAAG gamma_5_11 TGCGCCACTAAGGGACAAATTCCCC bacter_1_12 CGCGACTGTCATTCCACGTTCGAGC plancto_1_12 ACAGAGTTAGCCAGTGCTTCCTCTC gamma_5_12 TAAGGGACAAATTCCCCCAACGGCT bacter_1_13 GCGACTGTCATTCCACGTTCGAGCC plancto_1_13 ACCTGTGCAGGTCACACCCGAAGGT gamma_5_13 CTGTAGGTACCGTCAAGACGCGCAG bacter_1_14 CGACTGTCATTCCACGTTCGAGCCC plancto_1_14 CATGCAACACCTGTGCAGGTCACAC gamma_5_14 GTAGGTACCGTCAAGACGCGCAGTT bacter_1_15 TCCGCGACTGTCATTCCACGTTCGA plancto_1_15 CACCTGTGCAGGTCACACCCGAAGG gamma_5_15 CTGCGCCACTAAGGGACAAATTCCC bacter_1_16 GACTGTCATTCCACGTTCGAGCCCA plancto_1_16 CACAGAGTTAGCCAGTGCTTCCTCT gamma_5_16 TGTAGGTACCGTCAAGACGCGCAGT bacter_1_17 ATCACGTTTCCGCGACTGTCATTCC plancto_1_17 CAGAGTTAGCCAGTGCTTCCTCTCG gamma_5_17 TCTGTAGGTACCGTCAAGACGCGCA bacter_1_18 GTCATTCCACGTTCGAGCCCAGGTA plancto_1_18 AGCCAGTGCTTCCTCTCGAGCTTAC gamma_5_18 GTCCGCCACTCGACGCCTGAAGAGC bacter_1_19 ACGGTACCATCAGCACCGATACACG plancto_1_19 GCACAGAGTTAGCCAGTGCTTCCTC gamma_5_19 GCCACTCGACGCCTGAAGAGCAAGC bacter_1_20 GTACCATCAGCACCGATACACGACC plancto_1_20 GGCCTAGCCCCTGCATGTCAAGCCT gamma_5_20 GCTGCGCCACTAAGGGACAAATTCC bacter_1_21 GGTACCATCAGCACCGATACACGAC plancto_1_21 GCAGGTCACACCCGAAGGTAATCAG gamma_5_21 CACTCGGTTCCCGAAGGCACCAAAC bacter_1_22 CGGTACCATCAGCACCGATACACGA plancto_1_22 ACCGGCCTAGCCCCTGCATGTCAAG gamma_5_22 CTTCTGTAGGTACCGTCAAGACGCG bacter_1_23 GATCACGTTTCCGCGACTGTCATTC plancto_1_23 CAGGTCACACCCGAAGGTAATCAGC gamma_5_23 CACTCGACGCCTGAAGAGCAAGCTC bacter_1_24 TACGGTACCATCAGCACCGATACAC plancto_1_24 CCGGCCTAGCCCCTGCATGTCAAGC gamma_5_24 CGCCACTCGACGCCTGAAGAGCAAG bacter_1_25 CACCGATACACGACCGGTGGTTTTT plancto_1_25 CGGCCTAGCCCCTGCATGTCAAGCC gamma_5_25 GGACAAATTCCCCCAACGGCTAGTT bacter_2_1 GGATTTCTCCGGGCTACCTTCCGGT plancto_2_1 TCTCCGAAGAGCACTCTCCCCTTTC gamma_6_1 AGCTGCGCCACCAACCTCTTGAATG bacter_2_2 CTCCGGGCTACCTTCCGGTAAAGGG plancto_2_2 TACGACCGAGAAACTGTGGGAGGTC gamma_6_2 CCAACCTCTTGAATGAGGCCGACGG bacter_2_3 CGGATTTCTCCGGGCTACCTTCCGG plancto_2_3 ACCGAGAAACTGTGGGAGGTCCCTC gamma_6_3 TGCGCCACCAACCTCTTGAATGAGG bacter_2_4 TCTCCGGGCTACCTTCCGGTAAAGG plancto_2_4 CGACCGAGAAACTGTGGGAGGTCCC gamma_6_4 GCCACCAACCTCTTGAATGAGGCCG bacter_2_5 TTCTCCGGGCTACCTTCCGGTAAAG plancto_2_5 CTCCGAAGAGCACTCTCCCCTTTCA gamma_6_5 ACCAACCTCTTGAATGAGGCCGACG bacter_2_6 TTTCTCCGGGCTACCTTCCGGTAAA plancto_2_6 GCCCGACCTTCCTCTGAGGTTTGGT gamma_6_6 CTGCGCCACCAACCTCTTGAATGAG bacter_2_7 GATTTCTCCGGGCTACCTTCCGGTA plancto_2_7 AAACTGTGGGAGGTCCCTCGATCCA gamma_6_7 CAACCTCTTGAATGAGGCCGACGGC bacter_2_8 ATTTCTCCGGGCTACCTTCCGGTAA plancto_2_8 TCCGAAGAGCACTCTCCCCTTTCAG gamma_6_8 GCGCCACCAACCTCTTGAATGAGGC bacter_2_9 CCGGATTTCTCCGGGCTACCTTCCG plancto_2_9 GACCGAGAAACTGTGGGAGGTCCCT gamma_6_9 CGCCACCAACCTCTTGAATGAGGCC bacter_2_10 TCCGGATTTCTCCGGGCTACCTTCC plancto_2_10 ACGACCGAGAAACTGTGGGAGGTCC gamma_6_10 CACCAACCTCTTGAATGAGGCCGAC bacter_2_11 TCCGGGCTACCTTCCGGTAAAGGGT plancto_2_11 GAAACTGTGGGAGGTCCCTCGATCC gamma_6_11 GCTGCGCCACCAACCTCTTGAATGA bacter_2_12 ATCCGGATTTCTCCGGGCTACCTTC plancto_2_12 CTCTCCGAAGAGCACTCTCCCCTTT gamma_6_12 CCACCAACCTCTTGAATGAGGCCGA bacter_2_13 CTTTATGGATTAGCTCCCCGTCGCT plancto_2_13 GCCTGGAGGTAGGTATCTACCTGTT gamma_6_13 TAGCTGCGCCACCAACCTCTTGAAT bacter_2_14 ACTTTATGGATTAGCTCCCCGTCGC plancto_2_14 TCCCGACGCTATTCCCAGCCTGGAG gamma_6_14 AACCTCTTGAATGAGGCCGACGGCT bacter_2_15 CCGGGCTACCTTCCGGTAAAGGGTA plancto_2_15 TTGGGCATTACCGCCAGTTTCCCGA gamma_6_15 AGAGGTCCACTTTGCCCCGAAGGGC bacter_2_16 AATCCGGATTTCTCCGGGCTACCTT plancto_2_16 CCGAGAAACTGTGGGAGGTCCCTCG gamma_6_16 GAGGTCCACTTTGCCCCGAAGGGCG bacter_2_17 GCTACCTTCCGGTAAAGGGTAGGTT plancto_2_17 TGAGCAGACCCATCTCCAGGCGCCG gamma_6_17 TCTTCAGGTAACGTCAATACGCGCG bacter_2_18 GGCTACCTTCCGGTAAAGGGTAGGT plancto_2_18 AACTGTGGGAGGTCCCTCGATCCAG gamma_6_18 TTAGCTGCGCCACCAACCTCTTGAA bacter_2_19 GGGCTACCTTCCGGTAAAGGGTAGG plancto_2_19 CCCGACCTTCCTCTGAGGTTTGGTC gamma_6_19 CAGAGGTCCACTTTGCCCCGAAGGG bacter_2_20 TAATCCGGATTTCTCCGGGCTACCT plancto_2_20 TGGGCATTACCGCCAGTTTCCCGAC gamma_6_20 AGGTCCACTTTGCCCCGAAGGGCGT bacter_2_21 CTACCTTCCGGTAAAGGGTAGGTTG plancto_2_21 CGAGAAACTGTGGGAGGTCCCTCGA gamma_6_21 ACCTCTTGAATGAGGCCGACGGCTA bacter_2_22 CGGGCTACCTTCCGGTAAAGGGTAG plancto_2_22 GAGAAACTGTGGGAGGTCCCTCGAT gamma_6_22 CGCGCGGGTATTAACCGCACGCTTT bacter_2_23 TTAATCCGGATTTCTCCGGGCTACC plancto_2_23 CAGCCTGGAGGTAGGTATCTACCTG gamma_6_23 CTTCAGGTAACGTCAATACGCGCGG bacter_2_24 TTTATGGATTAGCTCCCCGTCGCTG plancto_2_24 AGCCCGACCTTCCTCTGAGGTTTGG gamma_6_24 TCAGAGGTCCACTTTGCCCCGAAGG bacter_2_25 TACCTTCCGGTAAAGGGTAGGTTGC plancto_2_25 AATAGTGAGCAGACCCATCTCCAGG gamma_6_25 ACGCGCGGGTATTAACCGCACGCTT bacter_3_1 GGCTCCTCGCCGTATCATCGAAATT plancto_3_1 CGCAGTGCCTCAGTTAAGCTCAGGC gamma_7_1 GTCCTCCGTAGTTAGACTAGCCACT bacter_3_2 CAACCTTGCCAATCACTCCCCAGGT plancto_3_2 GCAGTGCCTCAGTTAAGCTCAGGCA gamma_7_2 CGTCCTCCGTAGTTAGACTAGCCAC bacter_3_3 CTTGCCAATCACTCCCCAGGTGGAT plancto_3_3 CAACTCTGAGGGAGTACCCTCAGAG gamma_7_3 ACCGTCCTCCGTAGTTAGACTAGCC bacter_3_4 CAGGTAAGGCTCCTCGCCGTATCAT plancto_3_4 GTCAACTCTGAGGGAGTACCCTCAG gamma_7_4 CCGTCCTCCGTAGTTAGACTAGCCA bacter_3_5 AGGCTCCTCGCCGTATCATCGAAAT plancto_3_5 TATGTTTTCCTACGCCGTTCGCCGC gamma_7_5 GACCGTCCTCCGTAGTTAGACTAGC bacter_3_6 AACCTTGCCAATCACTCCCCAGGTG plancto_3_6 GCAGAAAGAGGAAACCTCCTCCCGC gamma_7_6 TGACCGTCCTCCGTAGTTAGACTAG bacter_3_7 ACCTTGCCAATCACTCCCCAGGTGG plancto_3_7 AACTCTGAGGGAGTACCCTCAGAGA gamma_7_7 CTGCAGGTAACGTCAAGTACTCACC bacter_3_8 TCAACCTTGCCAATCACTCCCCAGG plancto_3_8 TCAACTCTGAGGGAGTACCCTCAGA gamma_7_8 TATTAGGGGTAAGCCTTCCTCCCTG bacter_3_9 GGTAAGGCTCCTCGCCGTATCATCG plancto_3_9 CTATGTTTTCCTACGCCGTTGGCCG gamma_7_9 TGCAGGTAACGTCAAGTACTCACCC bacter_3_10 TCCGCCTACCCCAACTATACTCTAG plancto_3_10 TCCTATGTTTTCCTACGCCGTTCGC gamma_7_10 GCAGGTAACGTCAAGTACTCACCCG bacter_3_11 TTCAACCTTGCCAATCACTCCCCAG plancto_3_11 CCTATGTTTTCCTACGCCGTTCGCC gamma_7_11 TTCCCCGGGTTGTCCCCCACTCATG bacter_3_12 CCCAGGTAAGGCTCCTCGCCGTATC plancto_3_12 ACTCTGAGGGAGTACCCTCAGAGAT gamma_7_12 TCCCCGGGTTGTCCCCCACTCATGG bacter_3_13 AGGTAAGGCTCCTCGCCGTATCATC plancto_3_13 ACGCAGTGCCTCAGTTAAGCTCAGG gamma_7_13 CCCCGGGTTGTCCCCCACTCATGGG bacter_3_14 CCAATCACTCCCCAGGTGGATTACC plancto_3_14 TGTCAACTCTGAGGGAGTACCCTCA gamma_7_14 TTTCCCCGGGTTGTCCCCCACTCAT bacter_3_15 CCTTGCCAATCACTCCCCAGGTGGA plancto_3_15 ATGTTTTCCTACGCCGTTCGCCGCT gamma_7_15 CCCGGGTTGTCCCCCACTCATGGGT bacter_3_16 GTAAGGCTCCTCGCCGTATCATCGA plancto_3_16 AACGCAGTGCCTCAGTTAAGCTCAG gamma_7_16 CCGGGTTGTCCCCCACTCATGGGTA bacter_3_17 CCGCCTACCCCAACTATACTCTAGA plancto_3_17 CAGTGCCTCAGTTAAGCTCAGGCAT gamma_7_17 CTCACCCGTATTAGGGGTAAGCCTT bacter_3_18 CCAGGTAAGGCTCCTCGCCGTATCA plancto_3_18 CTGTCAACTCTGAGGGAGTACCCTC gamma_7_18 ACCCGTATTAGGGGTAAGCCTTCCT bacter_3_19 AAGGCTCCTCGCCGTATCATCGAAA plancto_3_19 CTCTGAGGGAGTACCCTCAGAGATT gamma_7_19 ACTCACCCGTATTAGGGGTAAGCCT bacter_3_20 GCCAATCACTCCCCAGGTGGATTAC plancto_3_20 TCTGTCAACTCTGAGGGAGTACCCT gamma_7_20 GTCAAGTACTCACCCGTATTAGGGG bacter_3_21 TAAGGCTCCTCGCCGTATCATCGAA plancto_3_21 GGAGTACCCTCAGAGATTTCATCCC gamma_7_21 TCACCCGTATTAGGGGTAAGCCTTC bacter_3_22 GCCCAGGTAAGGCTCCTCGCCGTAT plancto_3_22 CAAACGCAGTGCCTCAGTTAAGCTC gamma_7_22 CCCGTATTAGGGGTAAGCCTTCCTC bacter_3_23 CATTCCGCCTACCCCAACTATACTC plancto_3_23 CTCTGTCAACTCTGAGGGAGTACCC gamma_7_23 GTACTCACCCGTATTAGGGGTAAGC bacter_3_24 CAATCACTCCCCAGGTGGATTACCT plancto_3_24 ACAGCAGAAAGAGGAAACCTCCTCC gamma_7_24 CACCCGTATTAGGGGTAAGCCTTCC bacter_3_25 CCGCCGGAACTTTGATCATCAAGAG plancto_3_25 CTGAGGGAGTACCCTCAGAGATTTC gamma_7_25 TACTCACCCGTATTAGGGGTAAGCC flavo_1_1 CTCAGACACCAAGGTCCAAACAGCT plancto_4_1 ACTACCTAATATCGCATCGGCCGCT gamma_8_1 CGCGAGCTCATCCATCAGCACAAGG flavo_1_2 CAGACACCAAGGTCCAAACAGCTAG plancto_4_2 CAACTACCTAATATCGCATCGGCCG gamma_8_2 TCATCCATCAGCACAAGGTCCGAAG flavo_1_3 CACTCAGACACCAAGGTCCAAACAG plancto_4_3 AACTACCTAATATCGCATCGGCCGC gamma_8_3 CTCATCCATCAGCACAAGGTCCGAA flavo_1_4 GCTTAGCCACTCAGACACCAAGGTC plancto_4_4 CCAACTACCTAATATCGCATCGGCC gamma_8_4 GCTCATCCATCAGCACAAGGTCCGA flavo_1_5 ACTCAGACACCAAGGTCCAAACAGC plancto_4_5 ACGTTCCGATGTATTCCTACCCCGT gamma_8_5 ACGCGAGCTCATCCATCAGCACAAG flavo_1_6 CTTAGCCACTCAGACACCAAGGTCC plancto_4_6 TACGTTCCGATGTATTCCTACCCCG gamma_8_6 CATCCATCAGCACAAGGTCCGAAGA flavo_1_7 TACCGTCAAGCTTGGTACACGTACC plancto_4_7 GTACGTTCCGATGTATTCCTACCCC gamma_8_7 GACGCGAGCTCATCCATCAGCACAA flavo_1_8 GTACCGTCAAGCTTGGTACACGTAC plancto_4_8 CTACCTAATATCGCATCGGCCGCTC gamma_8_8 GCGAGCTCATCCATCAGCACAAGGT flavo_1_9 GCCACTCAGACACCAAGGTCCAAAC plancto_4_9 CGTTCCGATGTATTCCTACCCCGTT gamma_8_9 TCCATCAGCACAAGGTCCGAAGATC flavo_1_10 TTAGCCACTCAGACACCAAGGTCCA plancto_4_10 GTTTCCACCCACTAATCCGTGCATG gamma_8_10 CGACGCGAGCTCATCCATCAGCACA flavo_1_11 ACCGTCAAGCTTGGTACACGTACCA plancto_4_11 TTCCACCCACTAATCCGTGCATGTC gamma_8_11 CATCAGCACAAGGTCCGAAGATCCC flavo_1_12 CCACTCAGACACCAAGGTCCAAACA plancto_4_12 TCCACCCACTAATCCGTGCATGTCA gamma_8_12 CCCTCTAATGGGCAGATTCTCACGT flavo_1_13 AGCCACTCAGACACCAAGGTCCAAA plancto_4_13 CCACCCACTAATCCGTGCATGTCAA gamma_8_13 CCGACGCGAGCTCATCCATCAGCAC flavo_1_14 TAGCCACTCAGACACCAAGGTCCAA plancto_4_14 GGCAGTAAACCTTTGGTCTCTCGAC gamma_8_14 CCCCTCTAATGGGCAGATTCTCACG flavo_1_15 CCGTCAAGCTTGGTACACGTACCAA plancto_4_15 GGTACGTTCCGATGTATTCCTACCC gamma_8_15 CCCCCTCTAATGGGCAGATTCTCAC flavo_1_16 CGCTTAGCCACTCAGACACCAAGGT plancto_4_16 TGCGAGCGTCATGAATGTTTCCACC gamma_8_16 CGAGCTCATCCATCAGCACAAGGTC flavo_1_17 TCGCTTAGCCACTCAGACACCAAGG plancto_4_17 GCGAGCGTCATGAATGTTTCCACCC gamma_8_17 CCATCAGCACAAGGTCCGAAGATCC flavo_1_(—1)8 CGTCAAGCTTGGTACACGTACCAAG plancto_4_18 GAGCGTCATGAATGTTTCCACCCAC gamma_8_18 CCTCTAATGGGCAGATTCTCACGTG flavo_1_19 CAGCTAGTAACCATCGTTTACCGGC plancto_4_19 CGAGCGTCATGAATGTTTCCACCCA gamma_8_19 CCCAGGTTATCCCCCTCTAATGGGC flavo_1_20 GCCATAGCTAGAGACTATGGGGGAT plancto_4_20 CAGTTATGCCCCAGTGAATCGCCTT gamma_8_20 TCCGACGCGAGCTCATCCATCAGCA flavo_1_21 TGCCATAGCTAGAGACTATGGGGGA plancto_4_21 TCAGTTATGCCCCAGTGAATCGCCT gamma_8_21 GAGCTCATCCATCAGCACAAGGTCC flavo_1_22 ATGCCATAGCTAGAGACTATGGGGG plancto_4_22 AGTTATGCCCCAGTGAATCGCCTTC gamma_8_22 TTCCCCAGGTTATCCCCCTCTAATG flavo_1_23 TTCGCTTAGCCACTCAGACACCAAG plancto_4_23 GTCAGTTATGCCCCAGTGAATCGCC gamma_8_23 TCCCCAGGTTATCCCCCTCTAATGG flavo_1_24 AGCTAGTAACCATCGTTTACCGGCG plancto_4_24 GTTATGCCCCAGTGAATCGCCTTCG gamma_8_24 CCCCAGGTTATCCCCCTCTAATGGG flavo_1_25 GTCAAGCTTGGTACACGTACCAAGG plancto_4_25 CTCCACTGGATGTTCCATTCACCTC gamma_8_25 ATCCCCCTCTAATGGGCAGATTCTC flavo_2_1 TACAGTACCGTCAGAGCTCTACACG alpha_1_1 CCGGCCCCTTGCGGGAAGAAAGCCA gamma_9_1 CCTGTCCATCGGTTCCCGAAGGCAC flavo_2_2 TCTTACAGTACCGTCAGAGCTCTAC alpha_1_2 CACCTGTGCACCGGCCCCTTGCGGG gamma_9_2 CTGTCCATCGGTTCCCGAAGGCACC flavo_2_3 TTACAGTACCGTCAGAGCTCTACAC alpha_1_3 GCACCTGTGCACCGGCCCCTTGCGG gamma_9_3 TGTCCATCGGTTCCCGAAGGCACCA flavo_2_4 GCATACTCATCTCTTACCGCCGAAG alpha_1_4 CTGTGCACCGGCCCCTTGCGGGAAG gamma_9_4 CAGCACCTGTCCATCGGTTCCCGAA flavo_2_5 CATACTCATCTCTTACCGCCGAAGC alpha_1_5 ACCTGTGCACCGGCCCCTTGCGGGA gamma_9_5 AGCACCTGTCCATCGGTTCCCGAAG flavo_2_6 ACAGTACCGTCAGAGCTCTACACGT alpha_1_6 CCTGTGCACCGGCCCCTTGCGGGAA gamma_9_6 ACCTGTCCATCGGTTCCCGAAGGCA flavo_2_7 CAGTACCGTCAGAGCTCTACACGTA alpha_1_7 AGCACCTGTGCACCGGCCCCTTGCG gamma_9_7 GTCCATCGGTTCCCGAAGGCACCAA flavo_2_8 CTTACAGTACCGTCAGAGCTCTACA alpha_1_8 CGGCCCCTTGCGGGAAGAAAGCCAT gamma_9_8 CACCTGTCCATCGGTTCCCGAAGGC flavo_2_9 TACTCATCTCTTACCGCCGAAGCTT alpha_1_9 GCACCGGCCCCTTGCGGGAAGAAAG gamma_9_9 CCTCCCTCTCTCGCACTCTAGCCTT flavo_2_10 ATACTCATCTCTTACCGCCGAAGCT alpha_1_10 CACCGGCCCCTTGCGGGAAGAAAGC gamma_9_10 GCACCTGTCCATCGGTTCCCGAAGG flavo_2_11 CTCATCTCTTACCGCCGAAGCTTTA alpha_1_11 ACCGGCCCCTTGCGGGAAGAAAGCC gamma_9_11 GCAGCACCTGTCCATCGGTTCCCGA flavo_2_12 CGCCCAGTGGCTGCTCTCTGTCTAT alpha_1_12 TGTGCACCGGCCCCTTGCGGGAAGA gamma_9_12 ACCTCCCTCTCTCGCACTCTAGCCT flavo_2_13 CCAGTGGCTGCTCTCTGTCTATACC alpha_1_13 GTGCACCGGCCCCTTGCGGGAAGAA gamma_9_13 CTCCCTCTCTCGCACTCTAGCCTTC flavo_2_14 CCCAGTGGCTGCTCTCTGTCTATAC alpha_1_14 TGCACCGGCCCCTTGCGGGAAGAAA gamma_9_14 TCTCTCGCACTCTAGCCTTCCAGTA flavo_2_15 TCGCCCAGTGGCTGCTCTCTGTCTA alpha_1_15 CAGCACCTGTGCACCGGCCCCTTGC gamma_9_15 TCGCACTCTAGCCTTCCAGTATCGG flavo_2_16 GCCCAGTGGCTGCTCTCTGTCTATA alpha_1_16 TTGCGGGAAGAAAGCCATCTCTGGC gamma_9_16 CTCGCACTCTAGCCTTCCAGTATCG flavo_2_17 GACTCCGATCCGAACTGTGATATAG alpha_1_17 GGCCCCTTGCGGGAAGAAAGCCATC gamma_9_17 TACCTCCCTCTCTCGCACTCTAGCC flavo_2_18 AGAACGCATACTCATCTCTTACCGC alpha_1_18 CCTTGCGGGAAGAAAGCCATCTCTG gamma_9_18 CTCTCGCACTCTAGCCTTCCAGTAT flavo_2_19 GAACGCATACTCATCTCTTACCGCC alpha_1_19 GCAGCACCTGTGCACCGGCCCCTTG gamma_9_19 CCCTCTCTCGCACTCTAGCCTTCCA flavo_2_20 CACGTAGAGCGGTTTCTTCCTGTAT alpha_1_20 TGCGGGAAGAAAGCCATCTCTGGCG gamma_9_20 TGCAGCACCTGTCCATCGGTTCCCG flavo_2_21 GTCCTGTCACACTACATTTAAGCCC alpha_1_21 AAAGCCATCTCTGGCGATCATACCG gamma_9_21 ACTCCGTGGTAATCGCCCTCCCGAA flavo_2_22 ACTCATCTCTTACCGCCGAAGCTTT alpha_1_22 GCCCCTTGCGGGAAGAAAGCCATCT gamma_9_22 TCCATCGGTTCCCGAAGGCACCAAT flavo_2_23 CCCCTATCTATCGTAGCCATGGTGT alpha_1_23 AACAGCAAGCTGCCCAACGGCTAGC gamma_9_23 TCACTCCGTGGTAATCGCCCTCCCG flavo_2_24 CCCTATCTATCGTAGCCATGGTGTG alpha_1_24 CATGCAGCACCTGTGCACCGGCCCC gamma_9_24 TCCCTCTCTCGCACTCTAGCCTTCC flavo_2_25 CCTATCTATCGTAGCCATGGTGTGC alpha_1_25 GCAAGCTGCCCAACGGCTAGCATCC gamma_9_25 CCTCTCTCGCACTCTAGCCTTCCAG flavo_3_1 CTGTCACCTAACATTTAAGCCCTGG alpha_2_1 GTGACCCAGAAAGTTGCCTTCGCAT gamma_10_1 CGCAGGCACATCCGATAGCGAGAGC flavo_3_2 CCGTCAAGCTTTCTCACGAGAAAGT alpha_2_2 GTATTCACCGCGACGCGCTGATTCG gamma_10_2 ACGCAGGCACATCCGATAGCGAGAG flavo_3_3 ACCGTCAAGCTTTCTCACGAGAAAG alpha_2_3 CGTATTCACCGCGACGCGCTGATTC gamma_10_3 GCGGCTTCGCGGCCCTCTGTACTTG flavo_3_4 CTCTGACTTATTTGTCCACCTACGG alpha_2_4 TATTCACCGCGACGCGCTGATTCGC gamma_10_4 CGGCTTCGCGGCCCTCTGTACTTGC flavo_3_5 CCTCTGACTTATTTGTCCACCTACG alpha_2_5 ACGTATTCACCGCGACGCGCTGATT gamma_10_5 GGCTTCGCGGCCCTCTGTACTTGCC flavo_3_6 GTACCGTCAAGCTTTCTCACGAGAA alpha_2_6 GGAACGTATTCACCGCGACGCGCTG gamma_10_6 CGCGGCTTCGCGGCCCTCTGTACTT flavo_3_7 GAGGCAGATTGTATACGCGATACTC alpha_2_7 CCGGGAACGTATTCACCGCGACGCG gamma_10_7 GCTTCGCGGCCCTCTGTACTTGCCA flavo_3_8 TCTATCGTAGCCTAGGTGTGCCGTT alpha_2_8 CGGGAACGTATTCACCGCGACGCGC gamma_10_8 CACTACTGGGTAGTTTCCTACGCGT flavo_3_9 CCCCTATCTATCGTAGCCTAGGTGT alpha_2_9 GGGAACGTATTCACCGCGACGCGCT gamma_10_9 CCACTACTGGGTAGTTTCCTACGCG flavo_3_10 ATCTATCGTAGCCTAGGTGTGCCGT alpha_2_10 AACGTATTCACCGCGACGCGCTGAT gamma_10_10 CCCCACTACTGGGTAGTTTCCTACG flavo_3_11 CCCTATCTATCGTAGCCTAGGTGTG alpha_2_11 GAACGTATTCACCGCGACGCGCTGA gamma_10_11 CCCACTACTGGGTAGTTTCCTACGC flavo_3_12 TATCTATCGTAGCCTAGGTGTGCCG alpha_2_12 CCCGGGAACGTATTCACCGCGACGC gamma_10_12 CCCCCACTACTGGGTAGTTTCCTAC flavo_3_13 CCTATCTATCGTAGCCTAGGTGTGC alpha_2_13 ATTCACCGCGACGCGCTGATTCGCG gamma_10_13 ACTACCGGGTAGTTTCCTACGCGTT flavo_3_14 CTATCTATCGTAGCCTAGGTGTGCC alpha_2_14 CCGCGACGCGCTGATTCGCGATTAC gamma_10_14 CACTACCGGGTAGTTTCCTACGCGT flavo_3_15 CTATCGTAGCCTAGGTGTGCCGTTA alpha_2_15 CACCGCGACGCGCTGATTCGCGATT gamma_10_15 ACCGGGTAGTTTCCTACGCGTTACT flavo_3_16 TATCGTAGCCTAGGTGTGCCGTTAC alpha_2_16 CGCGACGCGCTGATTCGCGATTACT gamma_10_16 CCACTACCGGGTAGTTTCCTACGCG flavo_3_47 CTTATTTGTCCACCTACGGACCCTT alpha_2_17 TCACCGCGACGCGCTGATTCGCGAT gamma_10_17 CCCCACTACCGGGTAGTTTCCTACG flavo_3_18 ACTTATTTGTCCACCTACGGACCCT alpha_2_18 ACCGCGACGCGCTGATTCGCGATTA gamma_10_18 CCGGGTAGTTTCCTACGCGTTACTC flavo_3_19 GACTTATTTGTCCACCTACGGACCC alpha_2_19 GCGACGCGCTGATTCGCGATTACTA gamma_10_19 CCCACTACCGGGTAGTTTCCTACGC flavo_3_20 TGACTTATTTGTCCACCTACGGACC alpha_2_20 TTCACCGCGACGCGCTGATTCGCGA gamma_10_20 TACCGGGTAGTTTCCTACGCGTTAC flavo_3_21 CTGACTTATTTGTCCACCTACGGAC alpha_2_21 TCCTCAGTGTCAGTAGTGACCCAGA gamma_10_21 CCCCCACTACCGGGTAGTTTCCTAC flavo_3_22 AGATTGTATACGCGATACTCACCCG alpha_2_22 CCCAGAAAGTTGCCTTCGCATTTGG gamma_10_22 CTACCGGGTAGTTTCCTACGCGTTA flavo_3_23 GATTGTATACGCGATACTCACCCGT alpha_2_23 AGTGCGGGCTCATCTTTCGGCGTAT gamma_10_23 CTGTTGTCCCCCACTACTGGGTAGT flavo_3_24 TCTTCGGGCTATTCCCTAGTATGAG alpha_2_24 AAGTGCGGGCTCATCTTTCGGCGTA gamma_10_24 CTAGCTAATCTCACGCAGGCACATC flavo_3_25 CTTCGGGCTATTCCCTAGTATGAGG alpha_2_25 GTGCGGGCTCATCTTTCGGCGTATA gamma_10_25 CAACTAGCTAATCTCACGCAGGCAC flavo_4_1 CAGGAGATATTCCCATACTATGGGG alpha_3_1 CACCTGTATCCAATCCACCCGAAGT gamma_11_1 GCTTTCCCCCGTAGGATATATGCGG flavo_4_2 TCAAACTCCCACACGTGGGAGTGGT alpha_3_2 ACCTGTATCCAATCCACCCGAAGTG gamma_11_2 CTTTCCCCCGTAGGATATATGCGGT flavo_4_3 CAAACTCCCACACGTGGGAGTGGTT alpha_3_3 CCTGTATCCAATCCACCCGAAGTGA gamma_11_3 TGCTTTCCCCCGTAGGATATATGCG flavo_4_4 GTCAAACTCCCACACGTGGGAGTGG alpha_3_4 GCACCTGTATCCAATCCACCCGAAG gamma_11_4 CTGCTTTCCCCCGTAGGATATATGC flavo_4_5 GGAGATATTCCCATACTATGGGGCA alpha_3_5 GGCAGTTCCTTCAAAGTTCCCACCA gamma_11_5 CCTGCTTTCCCCCGTAGGATATATG flavo_4_6 AGGAGATATTCCCATACTATGGGGC alpha_3_6 AGCACCTGTATCCAATCCACCCGAA gamma_11_6 CCCTGCTTTCCCCCGTAGGATATAT flavo_4_7 CGTCAAACTCCCACACGTGGGAGTG alpha_3_7 CGGCAGTTCCTTCAAAGTTCCCACC gamma_11_7 CTCACTCAGGCTCATCAAATAGCGC flavo_4_8 AAACTCCCACACGTGGGAGTGGTTC alpha_3_2 CAGCACCTGTATCCAATCCACCCGA gamma_11_8 CCCCTGCTTTCCCCCGTAGGATATA flavo_4_9 CTGGGCTATTCCCCTCCAAAAGGTA alpha_3_9 CCGGCAGTTCCTTCAAAGTTCCCAC gamma_11_9 GTGTCAGTATCGAGCCAGTCAGTCG flavo_4_10 CCGTCAAACTCCCACACGTGGGAGT alpha_3_10 GCAGCACCTGTATCCAATCCACCCG gamma_11_10 TCAGTGTCAGTATCGAGCCAGTCAG flavo_4_11 CTTAACCACTCAGCCCTTAATCGGG alpha_3_11 TGCAGCACCTGTATCCAATCCACCC gamma_11_11 AGTGTCAGTATCGAGCCAGTCAGTC flavo_4_12 GTTTCCCTGGGCTATTCCCCTCCAA alpha_3_12 TCACCGGCAGTTCCTTCAAAGTTCC gamma_11_12 TGTCAGTATCGAGCCAGTCAGTCGC flavo_4_13 GCTTAACCACTCAGCCCTTAATCGG alpha_3_13 CTTACAAATCCGCCTACGCTCGCTT gamma_11_13 CAGTGTCAGTATCGAGCCAGTCAGT flavo_4_14 AACTCCCACACGTGGGAGTGGTTCT alpha_3_14 ATGCAGCACCTGTATCCAATCCACC gamma_11_14 CTCAGTGTCAGTATCGAGCCAGTCA flavo_4_15 ACCGTCAAACTCCCACACGTGGGAG alpha_3_15 CGGGCCCATCCAATAGCGCATAAAG gamma_11_15 TCCCCTGCTTTCCCCCGTAGGATAT flavo_4_16 CCACACGTGGGAGTGGTTCTTCCTC alpha_3_16 GGGCCCATCCAATAGCGCATAAAGC gamma_11_16 CCCCACCAACTAGCTAATCTCACTC flavo_4_17 AGTTTCCCTGGGCTATTCCCCTCCA alpha_3_17 GCGGGCCCATCCAATAGCGCATAAA gamma_11_17 CCTCAGTGTCAGTATCGAGCCAGTC flavo_4_18 TTAACCACTCAGCCCTTAATCGGGC alpha_3_18 ACTTACAAATCCGCCTACGCTCGCT gamma_11_18 GTCCCCTGCTTTCCCCCGTAGGATA flavo_4_19 CACGTGGGAGTGGTTCTTCCTCTGT alpha_3_19 CGCGGGCCCATCCAATAGCGCATAA gamma_11_19 TCAGTATCGAGCCAGTCAGTCGCCT flavo_4_20 CACACGTGGGAGTGGTTCTTCCTCT alpha_3_20 GGCCCATCCAATAGCGCATAAAGCT gamma_11_20 GTATCGAGCCAGTCAGTCGCCTTCG flavo_4_21 ACACGTGGGAGTGGTTCTTCCTCTG alpha_3_21 CACCGGCAGTTCCTTCAAAGTTCCC gamma_11_21 AGTATCGAGCCAGTCAGTCGCCTTC flavo_4_22 CGCTTAACCACTCAGCCCTTAATCG alpha_3_22 ACCGGCAGTTCCTTCAAAGTTCCCA gamma_11_22 TATCGAGCCAGTCAGTCGCCTTCGC flavo_4_23 ACGTGGGAGTGGTTCTTCCTCTGTA alpha_3_23 AACTTACAAATCCGCCTACGCTCGC gamma_11_23 ATCGAGCCAGTCAGTCGCCTTCGCC flavo_4_24 TTTCCCTGGGCTATTCCCCTCCAAA alpha_3_24 CGCATAAAGCTTTCTCCCGAAGGAC gamma_11_24 GTCAGTATCGAGCCAGTCAGTCGCC flavo_4_25 TTCCCTGGGCTATTCCCCTCCAAAA alpha_3_25 CATGCAGCACCTGTATCCAATCCAC gamma_11_25 CAGTATCGAGCCAGTCAGTCGCCTT flavo_5_1 CGTCAACAGTTCACACGTGAACCTT roseo_1_1 CTCTGGAATCCGCGACAAGTATGTC gamma_12_1 CACTACCTGGTAGATTCCTACGCGT flavo_5_2 ACAGTACCGTCAACAGTTCACACGT roseo_1_2 TGCCCCTATAAATAGTTGGCGCACC gamma_12_2 CCACTACCTGGTAGATTCCTACGCG flavo_5_3 CCGTCAACAGTTCACACGTGAACCT roseo_1_3 CCCTATAAATAGTTGGCGCACCACC gamma_12_3 CCCACTACCTGGTAGATTCCTACGC flavo_5_4 CAGTACCGTCAACAGTTCACACGTG roseo_1_4 CCCCTATAAATAGTTGGCGCACCAC gamma_12_4 AACTGTTGTCCCCCACTACCTGGTA flavo_5_5 TACAGTACCGTCAACAGTTCACACG roseo_1_5 GCCCCTATAAATAGTTGGCGCACCA gamma_12_5 CAACTGTTGTCCCCCACTACCTGGT flavo_5_6 ACCGTCAACAGTTCACACGTGAACC roseo_1_6 CGTGGTTGGCTGCCCCTATAAATAG gamma_12_6 CCAACTGTTGTCCCCCACTACCTGG flavo_5_2 CTACAGTACCGTCAACAGTTCACAC roseo_1_7 CTGCCCCTATAAATAGTTGGCGCAC gamma_12_7 CCCCACTACCTGGTAGATTCCTACG flavo_5_2 TACCGTCAACAGTTCACACGTGAAC roseo_1_8 CCGTGGTTGGCTGCCCCTATAAATA gamma_12_8 CGGTATTGCAACCCTCTGTACGCCC flavo_5_9 AGTACCGTCAACAGTTCACACGTGA roseo_1_9 TGGCTGCCCCTATAAATAGTTGGCG gamma_12_9 ACTGTTGTCCCCCACTACCTGGTAG flavo_5_10 GTACCGTCAACAGTTCACACGTGAA roseo_1_10 GGCTGCCCCTATAAATAGTTGGCGC gamma_12_10 TCCAACTGTTGTCCCCCACTACCTG flavo_5_11 CCTACAGTACCGTCAACAGTTCACA roseo_1_11 GGAATCCGCGACAAGTATGTCAAGG gamma_12_11 CCCCCACTACCTGGTAGATTCCTAC flavo_5_12 TCCTACAGTACCGTCAACAGTTCAC roseo_1_12 GCTGCCCCTATAAATAGTTGGCGCA gamma_12_12 GCGGTATTGCAACCCTCTGTACGCC flavo_5_13 CCGAAGAAAAAGATGTTTCCACCCC roseo_1_13 ACCGTGGTTGGCTGCCCCTATAAAT gamma_12_13 GCGGTATCGCAACCCTCTGTACGTT flavo_5_14 CTCAGACCGCAATTAGTCCGAACAG roseo_1_14 CCATCTCTGGAATCCGCGACAAGTA gamma_12_14 TCTATCAGTTTGGGGTGCAGTTCCC flavo_5_15 TAGCCACTCAGACCGCAATTAGTCC roseo_1_15 ATAGTTGGCGCACCACCTTCGGGTA gamma_12_15 GTCTATCAGTTTGGGGTGCAGTTCC flavo_5_16 TTAGCCACTCAGACCGCAATTAGTC roseo_1_16 GGAATCCATCTCTGGAATCCGCGAC gamma_12_16 CTGTTGTCCCCCACTACCTGGTAGA flavo_5_17 ACTCAGACCGCAATTAGTCCGAACA roseo_1_17 TACCGTGGTTGGCTGCCCCTATAAA gamma_12_17 CTATCAGTTTGGGGTGCAGTTCCCA flavo_5_18 AGATGTTTCCACCCCTGTCAAACTG roseo_1_18 GAATCCGCGACAAGTATGTCAAGGG gamma_12_18 CTGTTGCTAACGTCACAGCTAAGGG flavo_5_19 CAGACCGCAATTAGTCCGAACAGCT roseo_1_19 TCCATCTCTGGAATCCGCGACAAGT gamma_12_19 CAGTTTGGGGTGCAGTTCCCAGGTT flavo_5_20 GCCACTCAGACCGCAATTAGTCCGA roseo_1_20 ATCCATCTCTGGAATCCGCGACAAG gamma_12_20 AGTTTGGGGTGCAGTTCCCAGGTTG flavo_5_21 CACTCAGACCGCAATTAGTCCGAAC roseo_1_21 TAGTTGGCGCACCACCTTCGGGTAG gamma_12_24 TTCCAACTGTTGTCCCCCACTACCT flavo_5_22 CTTAGCCACTCAGACCGCAATTAGT roseo_1_22 CCTACCGTGGTTGGCTGCCCCTATA gamma_12_22 TATCAGTTTGGGGTGCAGTTCCCAG flavo_5_23 AGCCACTCAGACCGCAATTAGTCCG roseo_1_23 CTACCGTGGTTGGCTGCCCCTATAA gamma_12_23 CGGTATCGCAACCCTCTGTACGTTC flavo_5_24 TCAGACCGCAATTAGTCCGAACAGC roseo_1_24 ACGTCGTCCACACCTTCCTCCGGCT gamma_12_24 CCCCACCAACTAACTAATCTCACGC flavo_5_25 ACTTTCGCTTAGCCACTCAGACCGC roseo_1_25 GACGTCGTCCACACCTTCCTCCGGC gamma_12_25 GTCAGCGACTAGCAAGCTAGTCCTG flavo_6_1 AGTGCCGGAGTTAAGCCCCTGCATT roseo_2_1 GTCACCGGGTCACCGAAGTGAAAAC gamma_13_1 CGCCACTGAAAGACATTGTCTCCCA flavo_6_2 GTGCCGGAGTTAAGCCCCTGCATTT roseo_2_2 ACCGGGTCACCGAAGTGAAAACCAG gamma_13_2 GCGCCACTGAAAGACATTGTCTCCC flavo_6_3 CAGTGCCGGAGTTAAGCCCCTGCAT roseo_2_3 CACCGGGTCACCGAAGTGAAAACCA gamma_13_3 TGCGCCACTGAAAGACATTGTCTCC flavo_6_4 TGCCGGAGTTAAGCCCCTGCATTTC roseo_2_4 TCACCGGGTCACCGAAGTGAAAACC gamma_13_4 TGTCAGTACAGATCCAGGAGGCCGC flavo_6_5 AGTTAAGCCCCTGCATTTCACCACT roseo_2_5 TGTCACCGGGTCACCGAAGTGAAAA gamma_13_5 GTGTCAGTACAGATCCAGGAGGCCG flavo_6_6 GCAGTGCCGGAGTTAAGCCCCTGCA roseo_2_6 CCGGGTCACCGAAGTGAAAACCAGA gamma_13_6 CTGCGCCACTGAAAGACATTGTCTC flavo_6_7 GTTAAGCCCCTGCATTTCACCACTG roseo_2_7 AGATCTCTCTGGCGGTCCCGGGATG gamma_13_7 CTTGGCTCCAAAAGGCACACTCTCA flavo_6_2 GGCAGTGCCGGAGTTAAGCCCCTGC roseo_2_8 ACCAGATCTCTCTGGCGGTCCCGGG gamma_13_8 GAGAGCTTCAAGAGAGGCCCTCTTT flavo_6_9 TGGCAGTGCCGGAGTTAAGCCCCTG roseo_2_9 AACCAGATCTCTCTGGCGGTCCCGG gamma_13_9 CGAGAGCTTCAAGAGAGGCCCTCTT flavo_6_10 GAGTTAAGCCCCTGCATTTCACCAC roseo_2_10 AAACCAGATCTCTCTGGCGGTCCCG gamma_13_10 GCGAGAGCTTCAAGAGAGGCCCTCT flavo_6_11 GCCGGAGTTAAGCCCCTGCATTTCA roseo_2_11 TCTCTGGCGGTCCCGGGATGTCAAG gamma_13_11 TAGCGAGAGCTTCAAGAGAGGCCCT flavo_6_12 ATGGCAGTGCCGGAGTTAAGCCCCT roseo_2_12 ATCTCTCTGGCGGTCCCGGGATGTC gamma_13_12 AGAGCTTCAAGAGAGGCCCTCTTTC flavo_6_13 TTAAGCCCCTGCATTTCACCACTGA roseo_2_13 GATCTCTCTGGCGGTCCCGGGATGT gamma_13_13 AGCGAGAGCTTCAAGAGAGGCCCTC flavo_6_14 GGAGTTAAGCCCCTGCATTTCACCA roseo_2_14 CAGATCTCTCTGGCGGTCCCGGGAT gamma_13_14 GTCAGTACAGATCCAGGAGGCCGCC flavo_6_15 CGGAGTTAAGCCCCTGCATTTCACC roseo_2_15 TCTGGCGGTCCCGGGATGTCAAGGG gamma_13_15 TCAGTACAGATCCAGGAGGCCGCCT flavo_6_16 CCCTGCATTTCACCACTGACTTATC roseo_2_16 CTCTGGCGGTCCCGGGATGTCAAGG gamma_13_16 CAGTACAGATCCAGGAGGCCGCCTT flavo_6_17 CAATGGCAGTGCCGGAGTTAAGCCC roseo_2_17 CCAGATCTCTCTGGCGGTCCCGGGA gamma_13_17 AGTACAGATCCAGGAGGCCGCCTTC flavo_6_18 TCAATGGCAGTGCCGGAGTTAAGCC roseo_2_18 TCTCTCTGGCGGTCCCGGGATGTCA gamma_13_18 GCTGCGCCACTGAAAGACATTGTCT flavo_6_19 CCTTACGGTCACCGACTTCAGGCAC roseo_2_19 CTCTCTGGCGGTCCCGGGATGTCAA gamma_13_19 GAGCTTCAAGAGAGGCCCTCTTTCT flavo_6_20 CCGGAGTTAAGCCCCTGCATTTCAC roseo_2_20 CTGGCGGTCCCGGGATGTCAAGGGT gamma_13_20 TCTTGGCTCCAAAAGGCACACTCTC flavo_6_21 AATGGCAGTGCCGGAGTTAAGCCCC roseo_2_21 ACCTGTCACCGGGTCACCGAAGTGA gamma_13_21 AGTGTCAGTACAGATCCAGGAGGCC flavo_6_22 TATCAATGGCAGTGCCGGAGTTAAG roseo_2_22 CCTGTCACCGGGTCACCGAAGTGAA gamma_13_22 GGCCCTCTTTCTCCCTTAGGAGGTA flavo_6_23 GTATCAATGGCAGTGCCGGAGTTAA roseo_2_23 CTGTCACCGGGTCACCGAAGTGAAA gamma_13_23 AGCTTCAAGAGAGGCCCTCTTTCTC flavo_6_24 CCCCTGCATTTCACCACTGACTTAT roseo_2_24 CGGGTCACCGAAGTGAAAACCAGAT gamma_13_24 AGCTGCGCCACTGAAAGACATTGTC flavo_6_25 TAAGCCCCTGCATTTCACCACTGAC roseo_2_25 AAAACCAGATCTCTCTGGCGGTCCC gamma_13_25 CGAGAGCATCAAGAGAGGCCCTCTT flavo_7_1 TCTTACAGTACCGTCACCAGACTAC roseo_3_1 GCCGCTACACCCGAAGGTGCCGCTC gamma_14_1 GGCGGTCAACTTACTACGTTAGCTG flavo_7_2 CTTACAGTACCGTCACCAGACTACA roseo_3_2 CTACACCCGAAGGTGCCGCTCGACT gamma_14_2 CCAGGCGGTCAACTTACTACGTTAG flavo_7_3 CGTCACCAGACTACACGTAGTCCTT roseo_3_3 GCTACACCCGAAGGTGCCGCTCGAC gamma_14_3 GCGGTCAACTTACTACGTTAGCTGC flavo_7_4 GTACCGTCACCAGACTACACGTAGT roseo_3_4 CCGCTACACCCGAAGGTGCCGCTCG gamma_14_4 CAGGCGGTCAACTTACTACGTTAGC flavo_7_5 CCGTCACCAGACTACACGTAGTCCT roseo_3_5 CGCTACACCCGAAGGTGCCGCTCGA gamma_14_5 CCCAGGCGGTCAACTTACTACGTTA flavo_7_6 TACCGTCACCAGACTACACGTAGTC roseo_3_6 CGCCGCTACACCCGAAGGTGCCGCT gamma_14_6 CCGAGGGCACTGCTTCATTACAAAG flavo_7_7 ACCGTCACCAGACTACACGTAGTCC roseo_3_7 CCGCCGCTACACCCGAAGGTGCCGC gamma_14_7 CGAGGGCACTGCTTCATTACAAAGC flavo_7_8 TTACAGTACCGTCACCAGACTACAC roseo_3_8 TACACCCGAAGGTGCCGCTCGACTT gamma_14_8 TCCCGAGGGCACTGCTTCATTACAA flavo_7_9 GTCACCAGACTACACGTAGTCCTTA roseo_3_9 TCCGCCGCTACACCCGAAGGTGCCG gamma_14_9 CCCGAGGGCACTGCTTCATTACAAA flavo_7_10 TACAGTACCGTCACCAGACTACACG roseo_3_10 ACACCCGAAGGTGCCGCTCGACTTG gamma_14_10 CCCCAGGCGGTCAACTTACTACGTT flavo_7_11 ACAGTACCGTCACCAGACTACACGT roseo_3_11 GTCCGCCGCTACACCCGAAGGTGCC gamma_14_11 TCCCCAGGCGGTCAACTTACTACGT flavo_7_12 AACTTTCACCCCTGACTTAACAGCC roseo_3_12 ACCCGAAGGTGCCGCTCGACTTGCA gamma_14_12 CTCCCGAGGGCACTGCTTCATTACA flavo_7_13 CAGTACCGTCACCAGACTACACGTA roseo_3_13 CACCCGAAGGTGCCGCTCGACTTGC gamma_14_13 CTCCCCAGGCGGTCAACTTACTACG flavo_7_14 CCGGTCGTCAGCAAGAGCAAGCTCC roseo_3_14 CGTCCGCCGCTACACCCGAAGGTGC gamma_14_14 GCTCCCGAGGGCACTGCTTCATTAC flavo_7_15 ACTTTCACCCCTGACTTAACAGCCC roseo_3_15 CACCTGGTCTCTTACGAGAAAACCG gamma_14_15 TCTTGGCTCCCGAGGGCACTGCTTC flavo_7_16 CCCTGACTTAACAGCCCGCCTACGG roseo_3_16 CCAGGAGTTTTGGAGGCCGTTCCAG gamma_14_16 GGCTCCCGAGGGCACTGCTTCATTA flavo_7_17 TCGCTTGGCCGCTCAGATCGAAATC roseo_3_47 ACCTGGTCTCTTACGAGAAAACCGG gamma_14_47 TATCTTGGCTCCCGAGGGCACTGCT flavo_7_18 CGCTTGGCCGCTCAGATCGAAATCC roseo_3_18 CCGGATCTCTCCGGCGGTCCAGGGA gamma_14_18 ACTCCCCAGGCGGTCAACTTACTAC flavo_7_19 TTCGCTTGGCCGCTCAGATCGAAAT roseo_3_19 CCCGAAGGTGCCGCTCGACTTGCAT gamma_14_19 ATCTTGGCTCCCGAGGGCACTGCTT flavo_7_20 TTTCGCTTGGCCGCTCAGATCGAAA roseo_3_20 ACCAGGAGTTTTGGAGGCCGTTCCA gamma_14_20 TACTACGTTAGCTGCGCCACTGAGA flavo_7_21 GCTTGGCCGCTCAGATCGAAATCCA roseo_3_21 CAGGAGTTTTGGAGGCCGTTCCAGG gamma_14_21 GTATCTTGGCTCCCGAGGGCACTGC flavo_7_22 CTTGGCCGCTCAGATCGAAATCCAA roseo_3_22 CCGAAGGTGCCGCTCGACTTGCATG gamma_14_22 CTTGGCTCCCGAGGGCACTGCTTCA flavo_7_23 TTGGCCGCTCAGATCGAAATCCAAA roseo_3_23 CCGTCCGCCGCTACACCCGAAGGTG gamma_14_23 TGGCTCCCGAGGGCACTGCTTCATT flavo_7_24 GGCTATCCCTTAGTGTAAGGCAGAT roseo_3_24 AAACCGGATCTCTCCGGCGGTCCAG gamma_14_24 ACTACGTTAGCTGCGCCACTGAGAA flavo_7_25 GGGCTATCCCTTAGTGTAAGGCAGA roseo_3_25 CCTGGTCTCTTACGAGAAAACCGGA gamma_14_25 TTGGCTCCCGAGGGCACTGCTTCAT flavo_8_1 GCCGAAATACGGTACTACGGGGCAT roseo_4_1 CGTACCATCTCTGGTAGTAGCACAG gamma_15_1 TCCGTAGAAGTCCGGGCCGTGTCTC flavo_8_2 GATGCCGAAATACGGTACTACGGGG roseo_4_2 CCATCTCTGGTAGTAGCACAGGATG gamma_15_2 CCGTAGAAGTCCGGGCCGTGTCTCA flavo_8_3 ATGCCGAAATACGGTACTACGGGGC roseo_4_3 GTACCATCTCTGGTAGTAGCACAGG gamma_15_3 CGTAGAAGTCCGGGCCGTGTCTCAG flavo_8_4 TGCCGAAATACGGTACTACGGGGCA roseo_4_4 CTGGTAGTAGCACAGGATGTCAAGG gamma_15_4 GTAGAAGTCCGGGCCGTGTCTCAGT flavo_8_5 ACCGTATAACGATGCCGAAATACGG roseo_4_5 TGGTAGTAGCACAGGATGTCAAGGG gamma_15_5 TTCCGTAGAAGTCCGGGCCGTGTCT flavo_8_6 CCGTATAACGATGCCGAAATACGGT roseo_4_6 GAAGGGAACGTACCATCTCTGGTAG gamma_15_6 CTTCCGTAGAAGTCCGGGCCGTGTC flavo_8_7 CGATGCCGAAATACGGTACTACGGG roseo_4_7 CCTTAGAGAAGGGCATATTCCCACG gamma_15_7 TAGAAGTCCGGGCCGTGTCTCAGTC flavo_8_8 CCGAAATACGGTACTACGGGGCATT roseo_4_8 GGTAGTAGCACAGGATGTCAAGGGT gamma_15_8 ACTGCTGCCTTCCGTAGAAGTCCGG flavo_8_9 ACGATGCCGAAATACGGTACTACGG roseo_4_9 GGGAACGTACCATCTCTGGTAGTAG gamma_15_9 CATGCAGTCGAGTTCCAGACTGCAA flavo_8_10 AACGATGCCGAAATACGGTACTACG roseo_4_10 GGAACGTACCATCTCTGGTAGTAGC gamma_15_10 CCTCGAGCTATCCCCCTCCATTGGG flavo_8_11 CGAAGGAAAAGTCATCTCTGACCCT roseo_4_11 CGAAGGGAACGTACCATCTCTGGTA gamma_15_11 AGAAGTCCGGGCCGTGTCTCAGTCC flavo_8_12 CGAAATACGGTACTACGGGGCATTA roseo_4_12 CCGAAGGGAACGTACCATCTCTGGT gamma_15_12 TCCTCGAGCTATCCCCCTCCATTGG flavo_8_13 CCGAAGGAAAAGTCATCTCTGACCC roseo_4_13 CGTCCCCGAAGGGAACGTACCATCT gamma_15_13 CTCGAGCTATCCCCCTCCATTGGGT flavo_8_14 GTCATCTCTGACCCTGTCAATATGC roseo_4_14 CCCCGAAGGGAACGTACCATCTCTG gamma_15_14 TCATGCAGTCGAGTTCCAGACTGCA flavo_8_15 CCCGAAGGAAAAGTCATCTCTGACC roseo_4_15 GTCCCCGAAGGGAACGTACCATCTC gamma_15_15 CCTTCCGTAGAAGTCCGGGCCGTGT flavo_8_16 TACAAGGCAGGTTCCATACGCGGTG roseo_4_16 GCGTCCCCGAAGGGAACGTACCATC gamma_15_16 GCGCCACTGGATAAATCCAACGGCT flavo_8_17 GGCTTTAACCGTATAACGATGCCGA roseo_4_17 ACTGCGTCCCCGAAGGGAACGTACC gamma_15_17 TGCGCCACTGGATAAATCCAACGGC flavo_8_18 CTGGGCTATTCCCCTGTACAAGGCA roseo_4_18 CTGCGTCCCCGAAGGGAACGTACCA gamma_15_18 TTCCTCGAGCTATCCCCCTCCATTG flavo_8_19 GAAGGAAAAGTCATCTCTGACCCTG roseo_4_19 CCCGAAGGGAACGTACCATCTCTGG gamma_15_19 GTTCCAGACTGCAATTCGGACTACG flavo_8_20 GCCCGAAGGAAAAGTCATCTCTGAC roseo_4_20 TGCGTCCCCGAAGGGAACGTACCAT gamma_15_20 CCAGCTCGCGCTTTGGCAACCGTTT flavo_8_21 GTACAAGGCAGGTTCCATACGCGGT roseo_4_21 CTTAGAGAAGGGCATATTCCCACGC gamma_15_21 TCGAGCTATCCCCCTCCATTGGGTA flavo_8_22 TGTACAAGGCAGGTTCCATACGCGG roseo_4_22 GAAGGGCGCGCTCGACTTGCATGTA gamma_15_22 GCTGCGCCACTGGATAAATCCAACG flavo_8_23 CCTGGGCTATTCCCCTGTACAAGGC roseo_4_23 CACTGCGTCCCCGAAGGGAACGTAC gamma_15_23 CGCCACTGGATAAATCCAACGGCTA flavo_8_24 ACAAGGCAGGTTCCATACGCGGTGC roseo_4_24 TCACTGCGTCCCCGAAGGGAACGTA gamma_15_24 CTGCGCCACTGGATAAATCCAACGG flavo_8_25 GGCAGGTTCCATACGCGGTGCGCAC roseo_4_25 TCCCCGAAGGGAACGTACCATCTCT gamma_15_25 TTTCCTCGAGCTATCCCCCTCCATT flavo_9_1 ATTCCGCCTACTTCAATACAACTCA roseo_5_1 GTCACTATGTCCCGAAGGAAAGCCT gamma_16_1 TTTAAGGGTTTGGCTCCAGCTCGCG flavo_9_2 TTCCGCCTACTTCAATACAACTCAA roseo_5_2 CCGAAGGAAAGCCTGATCTCTCAGG gamma_16_2 TTTTAAGGGTTTGGCTCCAGCTCGC flavo_9_3 TATTCCGCCTACTTCAATACAACTC roseo_5_3 TGTCACTATGTCCCGAAGGAAAGCC gamma_16_3 TTAAGGGTTTGGCTCCAGCTCGCGC flavo_9_4 TCCGCCTACTTCAATACAACTCAAG roseo_5_4 TCCCGAAGGAAAGCCTGATCTCTCA gamma_16_4 GTTTTAAGGGTTTGGCTCCAGCTCG flavo_9_5 CATATTCCGCCTACTTCAATACAAC roseo_5_5 TCACTATGTCCCGAAGGAAAGCCTG gamma_16_5 CACGCGGTATACCTGGATCAGGGTT flavo_9_6 CCGCCTACTTCAATACAACTCAAGA roseo_5_6 CCCGAAGGAAAGCCTGATCTCTCAG gamma_16_6 ACACGCGGTATACCTGGATCAGGGT flavo_9_7 CGCCTACTTCAATACAACTCAAGAT roseo_5_7 CTGTCACTATGTCCCGAAGGAAAGC gamma_16_7 CTTCCTCCGGGTTTCACCCGGCAGT flavo_9_8 GAACTCAAGGTCCCGAACAGCTAGT roseo_5_8 GTCCCGAAGGAAAGCCTGATCTCTC gamma_16_8 TCCTCCGGGTTTCACCCGGCAGTCT flavo_9_9 TCAGAACTCAAGGTCCCGAACAGCT roseo_5_9 GCCTGATCTCTCAGGTTGTCATAGG gamma_16_9 CTTCACACACGCGGTATACCTGGAT flavo_9_10 ACTCAAGGTCCCGAACAGCTAGTAT roseo_5_10 TGACTGACTAATCCGCCTACGTACG gamma_16_10 CACACGCGGTATACCTGGATCAGGG flavo_9_11 GATGCCTATCAATAATACCATGAGG roseo_5_11 CTGACTGACTAATCCGCCTACGTAC gamma_16_11 ACACACGCGGTATACCTGGATCAGG flavo_9_12 AGAACTCAAGGTCCCGAACAGCTAG roseo_5_12 CGAAGGAAAGCCTGATCTCTCAGGT gamma_16_12 CACACACGCGGTATACCTGGATCAG flavo_9_13 CTCAAGGTCCCGAACAGCTAGTATC roseo_5_13 CACTATGTCCCGAAGGAAAGCCTGA gamma_16_13 CCTTCCTCCGGGTTTCACCCGGCAG flavo_9_14 AACTCAAGGTCCCGAACAGCTAGTA roseo_5_14 GCACCTGTCACTATGTCCCGAAGGA gamma_16_14 TTCCTCCGGGTTTCACCCGGCAGTC flavo_9_15 CAGAACTCAAGGTCCCGAACAGCTA roseo_5_15 CCTGTCACTATGTCCCGAAGGAAAG gamma_16_15 CCTCCGGGTTTCACCCGGCAGTCTC flavo_9_16 CTCAGAACTCAAGGTCCCGAACAGC roseo_5_16 CTATGTCCCGAAGGAAAGCCTGATC gamma_16_16 TTCACACACGCGGTATACCTGGATC flavo_9_17 TCAAGGTCCCGAACAGCTAGTATCC roseo_5_17 ATGTCCCGAAGGAAAGCCTGATCTC gamma_16_17 CGCCTTCCTCCGGGTTTCACCCGGC flavo_9_18 GCTCAGAACTCAAGGTCCCGAACAG roseo_5_18 AGCACCTGTCACTATGTCCCGAAGG gamma_16_18 CTCCGGGTTTCACCCGGCAGTCTCC flavo_9_19 CTACATATTCCGCCTACTTCAATAC roseo_5_19 CAGCACCTGTCACTATGTCCCGAAG gamma_16_19 GCGGTATACCTGGATCAGGGTTGCC flavo_9_20 GCCTACTTCAATACAACTCAAGATG roseo_5_20 CCTCCGAAGAGGTTAGCGCACGGCC gamma_16_20 CGGTATACCTGGATCAGGGTTGCCC flavo_9_21 TACACGTAAGGCTTATTCTTCCTGT roseo_5_21 TCCGCTGCCTCCTCCGAAGAGGTTA gamma_16_21 GGTATACCTGGATCAGGGTTGCCCC flavo_9_22 CACGTAAGGCTTATTCTTCCTGTAT roseo_5_22 CCGCTGCCTCCTCCGAAGAGGTTAG gamma_16_22 TCTTCACACACGCGGTATACCTGGA flavo_9_23 ACACGTAAGGCTTATTCTTCCTGTA roseo_5_23 TGTCCCGAAGGAAAGCCTGATCTCT gamma_16_23 TCACACACGCGGTATACCTGGATCA flavo_9_24 CTTAGCCGCTCAGAACTCAaGGTCC roseo_5_24 CACCTGTCACTATGTCCCGAAGGAA gamma_16_24 GCCTTCCTCCGGGTTTCACCCGGCA flavo_9_25 CGCTCAGAACTCAAGGTCCCGAACA roseo_5_25 GCAGCACCTGTCACTATGTCCCGAA gamma_16_25 CGCGGTATACCTGGATCAGGGTTGC flavo_10_1 CGCTTAGCCACTCATCTAACCAATG roseo_6_1 CGATAAAACCTAGTCTCCTAGGCGG gamma_17_1 GGCTCCTCCAATAGTGACCGGTCCG flavo_10_2 CTTTCGCTTAGCCACTCATCTAACC roseo_6_2 CCGAGGCTATTCCGAAGCAAAAGGT gamma_17_2 AGGCTCCTCCAATAGTGACCGGTCC flavo_10_3 ACACGTCGGAGTGTTTCTTCCTGTA roseo_6_3 CCCGAGGCTATTCCGAAGCAAAAGG gamma_17_3 CAGGCTCCTCCAATAGTGACCGGTC flavo_10_4 CCCGTGCGCCACTCGTCATCTGGTG roseo_6_4 AAAACCTAGTCTCCTAGGCGGTCAG gamma_17_4 CATGTATTAGGCCTGCCGCCAACGT flavo_10_5 ACCCGTGCGCCACTCGTCATCTGGT roseo_6_5 AAACCTAGTCTCCTAGGCGGTCAGA gamma_17_5 GCTCCTCCAATAGTGACCGGTCCGA flavo_10_6 CACCCGTGCGCCACTCGTCATCTGG roseo_6_6 TCCCGAGGCTATTCCGAAGCAAAAG gamma_17_6 GCAGGCTCCTCCAATAGTGACCGGT flavo_10_7 TACAACCCGTAGGGCTTTCATCCTG roseo_6_7 CTAGTCTCCTAGGCGGTCAGAGGAT gamma_17_7 CGCCTGAGAGCAAGCTCCCATCGTT flavo_10_8 ACAACCCGTAGGGCTTTCATCCTGC roseo_6_8 AACCTAGTCTCCTAGGCGGTCAGAG gamma_17_8 ACGCCTGAGAGCAAGCTCCCATCGT flavo_10_9 AACCCGTAGGGCTTTCATCCTGCAC roseo_6_9 CCTAGTCTCCTAGGCGGTCAGAGGA gamma_17_9 GCCTGAGAGCAAGCTCCCATCGTTT flavo_10_10 CAGTTTACAACCCGTAGGGCTTTCA roseo_6_10 TAGTCTCCTAGGCGGTCAGAGGATG gamma_17_10 GACGCCTGAGAGCAAGCTCCCATCG flavo_10_11 CAACCCGTAGGGCTTTCATCCTGCA roseo_6_11 CCTCTCAAACCAGCTACTGATCGCA gamma_17_11 AATCCTACGCAGGCTCCTCCAATAG flavo_10_12 TTACAACCCGTAGGGCTTTCATCCT roseo_6_12 TCCTCTCAAACCAGCTACTGATCGC gamma_17_12 GCATGTATTAGGCCTGCCGCCAACG flavo_10_13 AGCAGTTTACAACCCGTAGGGCTTT roseo_6_13 CTCTCAAACCAGCTACTGATCGCAG gamma_17_13 CTAATCCTACGCAGGCTCCTCCAAT flavo_10_14 GCAGTTTACAACCCGTAGGGCTTTC roseo_6_14 CTCAAACCAGCTACTGATCGCAGAC gamma_17_14 GCTAATCCTACGCAGGCTCCTCCAA flavo_10_15 AAGCAGTTTACAACCCGTAGGGCTT roseo_6_15 CAGCTACTGATCGCAGACTTGGTAG gamma_17_15 CGACGCCTGAGAGCAAGCTCCCATC flavo_10_16 CACGTCGGAGTGTTTCTTCCTGTAT roseo_6_16 CCAGCTACTGATCGCAGACTTGGTA gamma_17_16 CCTGAGAGCAAGCTCCCATCGTTTC flavo_10_17 TGCGCCACTCGTCATCTGGTGCAAG roseo_6_17 CCATGCAGCACCTGTCACTCTGTAT gamma_17_17 CTCCTCCAATAGTGACCGGTCCGAA flavo_10_18 CCGTGCGCCACTCGTCATCTGGTGC roseo_6_18 CATGCAGCACCTGTCACTCTGTATC gamma_17_18 ATCCTACGCAGGCTCCTCCAATAGT flavo_10_19 GCGCCACTCGTCATCTGGTGCAAGC roseo_6_19 AACCAGCTACTGATCGCAGACTTGG gamma_17_19 CGCAGGCTCCTCCAATAGTGACCGG flavo_10_20 CGTGCGCCACTCGTCATCTGGTGCA roseo_6_20 ACCAGCTACTGATCGCAGACTTGGT gamma_17_20 AGCTAATCCTACGCAGGCTCCTCCA flavo_10_21 GTGCGCCACTCGTCATCTGGTGCAA roseo_6_21 GCCATGCAGCACCTGTCACTCTGTA gamma_17_21 TCGACGCCTGAGAGCAAGCTCCCAT flavo_10_22 GTTTACAACCCGTAGGGCTTTCATC roseo_6_22 AGTTTCCCGAGGCTATTCCGAAGCA gamma_17_22 CTGAGAGCAAGCTCCCATCGTTTCC flavo_10_23 TTTACAACCCGTAGGGCTTTCATCC roseo_6_23 GTTTCCCGAGGCTATTCCGAAGCAA gamma_17_23 TGTATTAGGCCTGCCGCCAACGTTC flavo_10_24 GCACCCGTGCGCCACTCGTCATCTG roseo_6_24 GGCGGTCAGAGGATGTCAAGGGTTG gamma_17_24 TGCATGTATTAGGCCTGCCGCCAAC flavo_10_25 GCGAAGTGGCTGCTCTCTGTACCGG roseo_6_25 AGGCGGTCAGAGGATGTCAAGGGTT gamma_17_25 CGCCACCGGTATTCCTCAGAATATC flavo_11_1 GTACAAGTACTTTATGCTGCCCCTC alpha_4_1 CGACAGGCATGCCTGCCAACAACTA gamma_19_1 GAGGTTGCGACCCTTTGTCCTTCCC flavo_11_2 CCGCCGGAGCTTTTCTTAAAAACTC alpha_4_2 CCGACAGGCATGCCTGCCAACAACT gamma_19_2 GCGAGGTTGCGACCCTTTGTCCTTC flavo_11_3 CGGTCGCCATCAAAGTACAAGTACT alpha_4_3 ACCGACAGGCATGCCTGCCAACAAC gamma_19_3 CGAAACCTTTCAAGAAGAGGGCTCC flavo_11_4 CCGGTCGCCATCAAAGTACAAGTAC alpha_4_4 GACAGGCATGCCTGCCAACAACTAG gamma_19_4 AAAGTGGTGAGCGCCCAGATAAGCT flavo_11_5 CGTCCCTCAGCGTCAGTTAATTGTT alpha_4_5 CCGTCTGCCACTATATCGTTCGACT gamma_19_5 TGAGCGCCCAGATAAGCTACCCACT flavo_11_6 TACAAGTACTTTATGCTGCCCCTCG alpha_4_6 CACCGACAGGCATGCCTGCCAACAA gamma_19_6 CAAAGTGGTGAGCGCCCAGATAAGC flavo_11_7 CACGCGGCATCGCTGGATCAGAGTT alpha_4_7 CCCGTCTGCCACTATATCGTTCGAC gamma_19_7 GTGGTGAGCGCCCAGATAAGCTACC flavo_11_8 TCGTCCCTCAGCGTCAGTTAATTGT alpha_4_8 CAGGCATGCCTGCCAACAACTAGCT gamma_19_8 AGTGGTGAGCGCCCAGATAAGCTAC flavo_11_9 TCACGCGGCATCGCTGGATCAGAGT alpha_4_9 ACAGGCATGCCTGCCAACAACTAGC gamma_19_9 GTGAGCGCCCAGATAAGCTACCCAC flavo_11_10 TGCCAGTATCAAAGGCAGTTCTACC alpha_4_10 TCACCGACAGGCATGCCTGCCAACA gamma_19_10 GGTGAGCGCCCAGATAAGCTACCCA flavo_11_11 ACAAGTACTTTATGCTGCCCCTCGA alpha_4_11 GCATGCCTGCCAACAACTAGCTCTC gamma_19_11 TGGTGAGCGCCCAGATAAGCTACCC flavo_11_12 GTACATCGAACAGCTAGTGACCATC alpha_4_12 GGCATGCCTGCCAACAACTAGCTCT gamma_19_12 AAGTGGTGAGCGCCCAGATAAGCTA flavo_11_13 GCCAGTATCAAAGGCAGTTCTACCG alpha_4_13 CACCCGTCTGCCACTATATCGTTCG gamma_19_13 CGCCCAGATAAGCTACCCACTTCTT flavo_11_14 TTCGTCCCTCAGCGTCAGTTAATTG alpha_4_14 ACCCGTCTGCCACTATATCGTTCGA gamma_19_14 GCGCCCAGATAAGCTACCCACTTCT flavo_11_15 CAAGTACTTTATGCTGCCCCTCGAC alpha_4_15 GTCACCGACAGGCATGCCTGCCAAC gamma_19_15 GCGAAACCTTTCAAGAAGAGGGCTC flavo_11_16 CGCCGGTCGCCATCAAAGTACAAGT alpha_4_16 AGGCATGCCTGCCAACAACTAGCTC gamma_19_16 AGCGCCCAGATAAGCTACCCACTTC flavo_11_17 TCGCCGGTCGCCATCAAAGTACAAG alpha_4_17 CTCACCCGTCTGCCACTATATCGTT gamma_19_17 ACAAAGTGGTGAGCGCCCAGATAAG flavo_11_18 GCCGGTCGCCATCAAAGTACAAGTA alpha_4_18 TCACCCGTCTGCCACTATATCGTTC gamma_19_18 CACAAAGTGGTGAGCGCCCAGATAA flavo_11_19 TTCGCCGGTCGCCATCAAAGTACAA alpha_4_19 CATGCCTGCCAACAACTAGCTCTCA gamma_19_19 CGAGGTTGCGACCTTTGTCCTTCC flavo_11_20 CGTTCGCCGGTCGCCATCAAAGTAC alpha_4_20 CCTGCCAACAACTAGCTCTCATCGT gamma_19_20 GAGCGCCCAGATAAGCTACCCACTT flavo_11_21 GTTCGCCGGTCGCCATCAAAGTACA alpha_4_21 CGTCACCGACAGGCATGCCTGCCAA gamma_19_21 CGCGAGGTTGCGACCCTTTGTCCTT flavo_11_22 TACCTATCGGAGCTTAGGTGAGCCG alpha_4_22 CTCGGTATTCCGCTAACCTCTCCTG gamma_19_22 GACGCCTAAGAGCAAGCTCTTATCG flavo_11_23 TATCGGAGCTTAGGTGAGCCGTTAC alpha_4_23 ACTCACCCGTCTGCCACTATATCGT gamma_19_23 TCACAAAGTGGTGAGCGCCCAGATA flavo_11_24 CCCTGACTTAACAAACAGCCTGCGG alpha_4_24 GCGTCACCGACAGGCATGCCTGCCA gamma_19_24 GCAGGCTCATCTGATAGCGAAACCT flavo_11_25 ACCGTTGAGCGGTAGGATTTCACCC alpha_4_25 TACTCACCCGTCTGCCACTATATCG gamma_19_25 CGACGCCTAAGAGCAAGCTCTTATC flavo_12_1 CGTCTTCCTGCACGCTGCATGGCTG wolbach_1_1 GCCAGGACTTCTTCTGTGAGTACCG gamma_20_1 CCACTAAGGGACAAATTCCCCCAAC flavo_12_2 CCGTCTTCCTGCACGCTGCATGGCT wolbach_1_2 AGCCAGGACTTCTTCTGTGAGTACC gamma_20_2 CGCCACTAAGGGACAAATTCCCCCA flavo_12_3 GTCTTCCTGCACGCTGCATGGCTGG wolbach_1_3 CCAGGACTTCTTCTGTGAGTACCGT gamma_20_3 GCCACTAAGGGACAAATTCCCCCAA flavo_12_4 CTTCCTGCACGCTGCATGGCTGGAT wolbach_1_4 CGGAGTTAGCCAGGACTTCTTCTGT gamma_20_4 CACTAAGGGACAAATTCCCCCAACG flavo_12_5 TTCCTGCACGCTGCATGGCTGGATC wolbach_1_5 CCGGCCGAACCGACCCTATCCCTTC gamma_20_5 ACTAAGGGACAAATTCCCCCAACGG flavo_12_6 GCCGTCTTCCTGCACGCTGCATGGC wolbach_1_6 ACGGAGTTAGCCAGGACTTCTTCTG gamma_20_6 CTAAGGGACAAATTCCCCCAACGGC flavo_12_7 TCTTCCTGCACGCTGCATGGCrGGA wolbach_1_7 GGAGTTAGCCAGGACTTCTTCTGTG gamma_20_7 GCGCCACTAAGGGACAAATTCCCCC flavo_12_8 CACGCTGCATGGCTGGATCAGAGTT wolbach_1_8 CAGGACTTCTTCTGTGAGTACCGTC gamma_20_8 GGTACCGTCAAGACGCGCAGTTATT flavo_12_9 GGCCGTCTTCCTGCACGCTGCATGG wolbach_1_9 GGCACGGAGTTAGCCAGGACTTCTT gamma_20_9 AGGTACCGTCAAGACGCGCAGTTAT flavo_12_10 TGCCCACCTTTTACCACCGGAGTTT wolbach_1_10 CACGGAGTTAGCCAGGACTTCTTCT gamma_20_10 TAGGTACCGTCAAGACGCGCAGTTA flavo_12_11 ATGCCCACCTTTTACCACCGGAGTT wolbach_1_11 TGGCACGGAGTTAGCCAGGACTTCT gamma_20_11 TGCGCCACTAAGGGACAAATTCCCC flavo_12_12 CACACGTGGACAGATTTCTTCCTGT wolbach_1_12 GCACGGAGTTAGCCAGGACTTCTTC gamma_20_12 TAAGGGACAAATTCCCCCAACGGCT flavo_12_13 GAAGACTCGCTCTTCCTCGCGGAGT wolbach_1_13 CGCCTCAGCGTCAGATTTGAACCAG gamma_20_13 CTGTAGGTACCGTCAAGACGCGCAG flavo_12_14 CATGCCCACCTTTTACCACCGGAGT wolbach_1_14 GCGCCTCAGCGTCAGATTTGAACCA gamma_20_14 GTAGGTACCGTCAAGACGCGCAGTT flavo_12_15 CCGGCTTTGAAGACTCGCTCTTCCT wolbach_1_15 CTGGCACGGAGTTAGCCAGGACTTC gamma_20_15 CTGCGCCACTAAGGGACAAATTCCC flavo_12_16 CCACACGTGGACAGATTTCTTCCTG wolbach_1_16 CTGCTGGCACGGAGTTAGCCAGGAC gamma_20_16 TGTAGGTACCGTCAAGACGCGCAGT flavo_12_17 TTTGAAGACTCGCTCTTCCTCGCGG wolbach_1_17 GCTGGCACGGAGTTAGCCAGGACTT gamma_20_17 TCTGTAGGTACCGTCAAGACGCGCA flavo_12_18 GGCTTTGAAGACTCGCTCTTCCTCG wolbach_1_18 TGCTGGCACGGAGTTAGCCAGGACT gamma_20_18 GCTGCGCCACTAAGGGACAAATTCC flavo_12_19 CTTTGAAGACTCGCTCTTCCTCGCG wolbach_1_19 CGCGCCTCAGCGTCAGATTTGAACC gamma_20_19 CTTCTGTAGGTACCGTCAAGACGCG flavo_12_20 TGAAGACTCGCTCTTCCTCGCGGAG wolbach_1_20 GCCTTCGCGCCTCAGCGTCAGATTT gamma_20_20 TCTTCTGTAGGTACCGTCAAGACGC flavo_12_21 GACCGGCTTTGAAGACTCGCTCTTC wolbach_1_21 GCCTCAGCGTCAGATTTGAACCAGA gamma_20_21 GGACAAATTCCCCCAACGGCTAGTT flavo_12_22 CGGCTTTGAAGACTCGCTCTTCCTC wolbach_1_22 TCGCGCCTCAGCGTCAGATTTGAAC gamma_20_22 GACAAATTCCCCCAACGGCTAGTTG flavo_12_23 GCTTTGAAGACTCGCTCTTCCTCGC wolbach_1_23 CATGCAACACCTGTGTGAAACCCGG gamma_20_23 AGCTGCGCCACTAAGGGACAAATTC flavo_12_24 ACCGGCTTTGAAGACTCGCTCTTCC wolbach_1_24 GACTTTGCAGCCCATTGTAGCCACC gamma_20_24 CGTTACGCACCCGTCCGCCACTCGA flavo_12_25 TCGTACAGTACCGTCAACTACCCAC wolbach_1_25 CGACTTTGCAGCCCATTGTAGCCAC gamma_20_25 TCGCGTTAGCTGCGCCACTAAGGGA flavo_13_1 CGCCGGTCGTCAGCATAGCAAGCTA rickett_1_1 TCTCTGCGATCCGCGACCACCATGT gamma_21_1 TCGTCAGCGCAGAGCAAGCTCCGCC flavo_13_2 AGGTCGCTCCTCACGGTAACGAACT rickett_1_2 ATCTCTGCGATCCGCGACCACCATG gamma_21_2 CTCGTCAGCGCAGAGCAAGCTCCGC flavo_13_3 GGTCGCTCCTCACGGTAACGAACTT rickett_1_3 GTCAGTTGTAGCCCAGATGACCGCC gamma_21_3 ACTCGTCAGCGCAGAGCAAGCTCCG flavo_13_4 TAGGTCGCTCCTCACGGTAACGAAC rickett_1_4 CAGTTGTAGCCCAGATGACCGCCTT gamma_21_4 AGCAAGCTCCGCCTGTTACCGTTCG flavo_13_5 AGGACGCATAGTCATCTTGTACCCA rickett_1_5 TCAGTTGTAGCCCAGATGACCGCCT gamma_21_5 GTCAGCGCAGAGCAAGCTCCGCCTG flavo_13_6 CCTCACGGTAACGAACITCAGGCAC rickett_1_6 CGTCAGTTGTAGCCCAGATGACCGC gamma_21_6 GAGCAAGCTCCGCCTGTTACCGTTC flavo_13_7 TCGCCCAGTGGCTGCTCATTGTCCA rickett_1_7 GTTGTAGCCCAGATGACCGCCTTCG gamma_21_7 CAAGCTCCGCCTGTTACCGTTCGAC flavo_13_8 CGTTCGCCGGTCGTCAGCATAGCAA rickett_1_8 AGTTGTAGCCCAGATGACCGCCTTC gamma_21_8 GCTCCGCCTGTTACCGTTCGACTTG flavo_13_9 GTCGCTCCTCACGGTAACGAACTTC rickett_1_9 CATCTCTGCGATCCGCGACCACCAT gamma_21_9 CTGGGCTTTCACATCCGACTGACCG flavo_13_10 GTCGCCCAGTGGCTGCTCATTGTCC rickett_1_10 GCGTCAGTTGTAGCCCAGATGACCG gamma_21_10 CTTTTGCAAGCCACTCCCATGGTGT flavo_13_11 TAGGACGCATAGTCATCTTGTACCC rickett_1_11 AGCATCTCTGCGATCCGCGACCACC gamma_21_11 TCTTTTGCAAGCCACTCCCATGGTG flavo_13_12 ACCAGTATCAAAGGCAGTTCCATCG rickett_1_12 GCATCTCTGCGATCCGCGACCACCA gamma_21_12 CTTCTTTTGCAAGCCACTCCCATGG flavo_13_13 TCCTCACGGTAACGAACTTCAGGCA rickett_1_13 TTGTAGCCCAGATGACCGCCTTCGC gamma_21_13 TTTTGCAAGCCACTCCCATGGTGTG flavo_13_14 CTAGGTCGCTCCTCACGGTAACGAA rickett_1_14 AGCGTCAGTTGTAGCCCAGATGACC gamma_21_14 TTTGCAAGCCACTCCCATGGTGTGA flavo_13_15 CTCCTCACGGTAACGAACTTCAGGC rickett_1_15 CCACTAACTAATTGGAGCAAGCCCC gamma_21_15 CCTCAGCGTCAGTATTGCTCCAGAA flavo_13_16 CCGTTCGCCGGTCGTCAGCATAGCA rickett_1_16 GCCACTAACTAATTGGAGCAAGCCC gamma_21_16 GGGCTTTCACATCCGACTGACCGTG flavo_13_17 GTTCGCCGGTCGTCAGCATAGCAAG rickett_1_17 CAAGCCCCAATTAGTCCGTTCGACT gamma_21_17 CTTTCACATCCGACTGACCGTGCCG flavo_13_18 CTCACGGTAACGAACTTCAGGCACT rickett_1_18 CCGTCTTGCTTCCCTCTGTAAACAC gamma_21_18 GGCTTTCACATCCGACTGACCGTGC flavo_13_19 TCGCrCCTCACGGTAACGAACTTCA rickett_1_19 CCGTCTGCCACTAACTAATTGGAGC gamma_21_19 CACTCGTCAGCGCAGAGCAAGCTCC flavo_13_20 GGTCGCCCAGTGGCTGCTCATTGTC rickett_1_20 CTCTGCGATCCGCGACCACCATGTC gamma_21_20 GCTTTCACATCCGACTGACCGTGCC flavo_13_21 CGGCATAGCTGGTTCAGAGTTGCCT rickett_1_21 GCAAGCCCCAATTAGTCCGTTCGAC gamma_21_21 TCAGCGCAGAGCAAGCTCCGCCTGT flavo_13_22 GGCATAGCTGGTTCAGAGTTGCCTC rickett_1_22 AGCAAGCCCCAATTAGTCCGTTCGA gamma_21_22 CGTCAGCGCAGAGCAAGCTCCGCCT flavo_13_23 CGCGGCATAGCTGGTTCAGAGTTGC rickett_1_23 TGTAGCCCAGATGACCGCCTTCGCC gamma_21_23 AGAGCAAGCTCCGCCTGTTACCGTT flavo_13_24 GCGGCATAGCTGGTTCAGAGTTGCC rickett_1_24 GAGCAAGCCCCAATTAGTCCGTTCG gamma_21_24 AGCTCCGCCTGGTACCGTTCGACTT flavo_13_25 GCATAGCTGGTTCAGAGTTGCCTCC rickett_1_25 GAAGAAAAGCATCTCTGCGATCCGC gamma_21_25 CAGAGCAAGCTCCGCCTGTTACCGT flavo_14_1 GTGCAAGCACTCCTGTTACCCCTCG alpha_5_1 ACCAAAGCCCTGTGGGCCCTAGCAG verru_1_1 CCCCGAGATTTCACACCTCACACAT flavo_14_2 AGTGCAAGCACTCCTGTTACCCCTC alpha_5_2 CACCAAAGCCCTGTGGGCCCTAGCA verru_1_2 CCCGAGATTTCACACCTCACACATC flavo_14_3 GCAAGCACTCCTGTTACCCCTCGAC alpha_5_3 CCAAAGCCCTGTGGGCCCTAGCAGC verru_1_3 TCACACCTCACACATCTATCCGCCT flavo_14_4 TGCAAGCACTCCTGTTACCCCTCGA alpha_5_4 ACCCTATGGTAGATCCCCACGCGTT verru_1_4 CACCTCACACATCTATCCGCCTACG flavo_14_5 CAAGCACTCCTGTTACCCCTCGACT alpha_5_5 CACCCTATGGTAGATCCCCACGCGT verru_1_5 TTCACACCTCACACATCTATCCGCC flavo_14_6 AAGCACTCCTGTTACCCCTCGACTT alpha_5_6 GCACCCTATGGTAGATCCCCACGCG verru_1_6 ACACCTCACACATCTATCCGCCTAC flavo_14_7 AGCACTCCTGTTACCCCTCGACTTG alpha_5_7 CCGCACCCTATGGTAGATCCCCACG verru_1_7 CACACCTCACACATCTATCCGCCTA flavo_14_8 GCACTCCTGTTACCCCTCGACTTGC alpha_5_8 CGCACCCTATGGTAGATCCCCACGC verru_1_8 GCCCCGAGATTTCACACCTCACACA flavo_14_9 TGCTACACGTAGCAGTGTTTCTTCC alpha_5_9 TATTCCGCACCCTATGGTAGATCCC verru_1_9 ACCTCACACATCTATCCGCCTACGC flavo_14_10 CCCGTGCGCCGGTCGTCAGCGAGTG alpha_5_10 ATTCCGCACCCTATGGTAGATCCCC verru_1_10 AGCCCCGAGATTTCACACCTCACAC flavo_14_11 TCGTCAGCGAGTGCAAGCACTCCTG alpha_5_11 TCCGCACCCTATGGTAGATCCCCAC verru_1_11 CTCCCGAAGGATAGCTCACGTACTT flavo_14_12 TGCGCCGGTCGTCAGCGAGTGCAAG alpha_5_12 CGCACCAGCTTCGGGTTGATCCAAC verru_1_12 CTGCCTCCCGAAGGATAGCTCACGT flavo_14_13 CGGTCGTCAGCGAGTGCAAGCACTC alpha_5_13 TTCCGCACCCTATGGTAGATCCCCA verru_1_13 GGCTATGAACCTCCTTGTTGCTCCT flavo_14_14 CCGTGCGCCGGTCGTCAGCGAGTGC alpha_5_14 CCACCAAAGCCCTGTGGGCCCTAGC verru_1_14 CCTCCCGAAGGATAGCTCACGTACT flavo_14_15 GCGCCGGTCGTCAGCGAGTGCAAGC alpha_5_15 CCCTATGGTAGATCCCCACGCGTTA verru_1_15 CCCGAAGGATAGCTCACGTACTTCG flavo_14_16 GGTCGTCAGCGAGTGCAAGCACTCC alpha_5_16 CCTATGGTAGATCCCCACGCGTTAC verru_1_16 TCCCGAAGGATAGCTCACGTACTTC flavo_14_17 GCCGGTCGTCAGCGAGTGCAAGCAC alpha_5_17 GCGCACCAGCTTCGGGTTGATCCAA verru_1_17 GAGGCTATGAACCTCCTTGTTGCTC flavo_14_18 GTCAGCGAGTGCAAGCACTCCTGTT alpha_5_18 GCACCAGCTTCGGGTTGATCCAACT verru_1_18 GACGCTGCCTCCCGAAGGATAGCTC flavo_14_19 CCGGTCGTCAGCGAGTGCAAGCACT alpha_5_19 AGCGCACCAGCTTCGGGTTGATCCA verru_1_19 AGGCTATGAACCTCCTTGTTGCTCC flavo_14_20 TCAGCGAGTGCAAGCACTCCTGTTA alpha_5_20 CTATGGTAGATCCCCACGCGTTACG verru_1_20 GCCTCCCGAAGGATAGCTCACGTAC flavo_14_21 CGTGCGCCGGTCGTCAGCGAGTGCA alpha_5_21 GCCACCAAAGCCCTGTGGGCCCTAG verru_1_21 CGCTGCCTCCCGAAGGATAGCTCAC flavo_14_22 CGCCGGTCGTCAGCGAGTGCAAGCA alpha_5_22 CACCAGCTTCGGGTTGATCCAACTC verru_1_22 TGCCTCCCGAAGGATAGCTCACGTA flavo_14_23 GTGCGCCGGTCGTCAGCGAGTGCAA alpha_5_23 TAGCGCACCAGCTTCGGGTTGATCC verru_1_23 ACGCTGCCTCCCGAAGGATAGCTCA flavo_14_24 CGTCAGCGAGTGCAAGCACTCCTGT alpha_5_24 CAAAGCCCTGTGGGCCCTAGCAGCT verru_1_24 GCTGCCTCCCGAAGGATAGCTCACG flavo_14_25 GTCGTCAGCGAGTGCAAGCACTCCT alpha_5_25 CGCCACCAAAGCCCTGTGGGCCCTA verru_1_25 AGGACGCTGCCTCCCGAAGGATAGC flavo_15_1 GGCGTACTCCCCAGGTGCATCACTT alpha_6_1 GCGCCACTAACCCCGAAGCTTCGTT verru_2_1 CGTCGCATGTTCACACTTTCGTGCA flavo_15_2 CTCCCCAGGTGCATCACTTAATACT alpha_6_2 CTTCTTGCGAGTAGCTGCCCACTGT verru_2_2 CTACCCTAACTTTCGTCCATGAGCG flavo_15_3 GCGTACTCCCCAGGTGCATCACTTA alpha_6_3 CCCAGCTTGTTGGGCCATGAGGACT verru_2_3 ACCCTAACTTTCGTCCATGAGCGTC flavo_15_4 CGGCGTACTCCCCAGGTGCATCACT alpha_6_4 ATCTTCTTGCGAGTAGCTGCCCACT verru_2_4 GCGTCGCATGTTCACACTTTCGTGC flavo_15_5 ACTCCCCAGGTGCATCACTTAATAC alpha_6_5 TCTTCTTGCGAGTAGCTGCCCACTG verru_2_5 CAAGTGTTCCCTTCTCCCCTCCAGT flavo_15_6 CGTACTCCCCAGGTGCATCACTTAA alpha_6_6 TAGCCCAGCTTGTTGGGCCATGAGG verru_2_6 TACACCAAGTGTTCCCTTCTCCCCT flavo_15_7 CCGGCGTACTCCCCAGGTGCATCAC alpha_6_7 GCCACTAACCCCGAAGCTTCGTTCG verru_2_7 CCAAGTGTTCCCTTCTCCCCTCCAG flavo_15_8 GTACTCCCCAGGTGCATCACTTAAT alpha_6_8 GTAGCCCAGCTTGTTGGGCCATGAG verru_2_8 ACACCAAGTGTTCCCTTCTCCCCTC flavo_15_9 GCCGGCGTACTCCCCAGGTGCATCA alpha_6_9 CGCCACTAACCCCGAAGCTTCGTTC verru_2_9 CGCTACACCAAGTGTTCCCTTCTCC flavo_15_10 GAAGAGAAGGCCTGTTTCCAAGCCG alpha_6_10 TTCTTGCGAGTAGCTGCCCACTGTC verru_2_10 CACCAAGTGTTCCCTTCTCCCCTCC flavo_15_11 CAACAGCGAGTGATGATCGTTTACG alpha_6_11 TAGCATCTTCTTGCGAGTAGCTGCC verru_2_11 GCTACACCAAGTGTTCCCTTCTCCC flavo_15_12 GCATGCCCATCTCATACCGAAAAAC alpha_6_12 AGCATCTTCTTGCGAGTAGCTGCCC verru_2_12 CTACACCAAGTGTTCCCTTCTCCCC flavo_15_13 TTGTAATCTGCTCCGAAGAGAAGGC alpha_6_13 GCCCAGCTTGTTGGGCCATGAGGAC verru_2_13 AGTGTTCCCTTCTCCCCTCCAGTAC flavo_15_14 CGCCGGTCGTCAGCAAAAGCAAGCT alpha_6_14 CACTAACCCCGAAGCTTCGTTCGAC verru_2_14 AAGTGTTCCCTTCTCCCCTCCAGTA flavo_15_15 AAGAGAAGGCCTGTTTCCAAGCCGG alpha_6_15 CATCTTCTTGCGAGTAGCTGCCCAC verru_2_15 ACCAAGTGTTCCCTTCTCCCCTCCA flavo_15_16 GCCGGTCGTCAGCAAAAGCAAGCTT alpha_6_16 TGTAGCCCAGCTTGTTGGGCCATGA verru_2_16 GCTACCCTAACTTTCGTCCATGAGC flavo_15_17 TGCCGGCGTACTCCCCAGGTGCATC alpha_6_17 AGCCCAGCTTGTTGGGCCATGAGGA verru_2_17 GTTCCCTTCTCCCCTCCAGTACTCT flavo_15_18 GCGCCGGTCGTCAGCAAAAGCAAGC alpha_6_18 CCACTAACCCCGAAGCTTCGTTCGA verru_2_18 GTGTTCCCTTCTCCCCTCCAGTACT flavo_15_19 CGAAGAGAAGGCCTGTTTCCAAGCC alpha_6_19 GCATCTTCTTGCGAGTAGCTGCCCA verru_2_19 TGTTCCCTTCTCCCCTCCAGTACTC flavo_15_20 CCAACAGCGAGTGATGATCGTTTAC alpha_6_20 GTGTAGCCCAGCTTGTTGGGCCATG verru_2_20 CCGCTACACCAAGTGTTCCCTTCTC flavo_15_21 GGAGTATTAATCCCCGTTTCCAGGG alpha_6_21 TGCGCCACTAACCCCGAAGCTTCGT verru_2_21 TTCCCTTCTCCCCTCCAGTACTCTA flavo_15_22 TGGAGTATTAATCCCCGTTTCCAGG alpha_6_22 CTCAAGCACCAAGTGCCCGAACAGC verru_2_22 GGCGTCGCATGTTCACACTTTCGTG flavo_15_23 TCCCCGTTTCCAGGGGCTATCCTCC alpha_6_23 CCAGCTTGTTGGGCCATGAGGACTT verru_2_23 CGCTACCCTAACTTTCGTCCATGAG flavo_15_24 TGCGCCGGTCGTCAGCAAAAGCAAG alpha_6_24 ACTAACCCCGAAGCTTCGTTCGACT verru_2_24 CCCTAACTTTCGTCCATGAGCGTCA flavo_15_25 AACAGCGAGTGATGATCGTTTACGG alpha_6_25 TCTTGCGAGTAGCTGCCCACTGTCA verru_2_25 ACCGCTACACCAAGTGTTCCCTTCT 

What is claimed is:
 1. A method for determination of stable isotope incorporation in a community of organisms comprising the steps of: a) supplying said community of organisms with at least two substrates simultaneously for a defined period of time, wherein each of the at least two substrates is labeled with a different stable isotope; b) extracting RNA from the organisms; c) fragmenting said RNA to provide fragmented RNA; d) labeling a fraction of the fragmented RNA with a detectable label to provide labeled fragmented RNA; e) hybridizing the labeled fragmented RNA to a set of oligonucleotide probes, wherein the set of oligonucleotide probes is an array of oligonucleotide probes attached to a substrate; f) detecting hybridization signal strength of labeled fragmented RNA hybridized to the oligonucleotide probes to determine the community organism composition; g) identifying a responsive set of oligonucleotide probes based on the hybridization signal strength in step f); h) hybridizing a fraction of unlabeled fragmented RNA to a second array of oligonucleotide probes, wherein the second array comprises the responsive set of oligonucleotide probes attached to a conductive substrate; i) detecting the unlabeled fragmented RNA hybridized to the responsive set of probes to determine the stable isotope incorporation into the community of organisms using imaging mass spectrometry or spectroscopy.
 2. The method of claim 1, wherein said organism is a bacterium, archaea, virus, fungus, plant, arthropod, nematode, or other eukaryote.
 3. The method of claim 2, wherein said organism is a bacterium.
 4. The method of claim 1, wherein the stable isotopes are selected from the group consisting of ³H, ¹³C, ¹⁵N, and ¹⁸O.
 5. The method of claim 1, wherein in step b) the extracting step is carried out by physical or chemical cell lysis followed by affinity column purification.
 6. The method of claim 1, wherein in step c) the fragmenting step is carried out by using enzymes, chemicals, or heat, or a combination of these.
 7. The method of claim 1, wherein in step d) the RNA is labeled with a fluorescent molecule or a non-fluorescent molecule.
 8. The method of claim 1, wherein steps c) and d) are carried out concurrently.
 9. The method of claim 1, wherein step e) further comprises the steps of adding said labeled fragmented RNA to a hybridization solution and contacting said hybridization solution with the array of oligonucleotide probes.
 10. The method of claim 1, wherein the set of oligonucleotide probes comprises 16S rRNA phylogenetic oligonucleotide probes.
 11. The method of claim 10, wherein said set of 16S rRNA phylogenetic probes further comprises probes from the 16S rRNA gene, 23S rRNA gene, 5S rRNA gene, 5.8S rRNA gene, 12S rRNA gene, 18S rRNA gene, 28S rRNA gene, gyrB gene, rpoB gene, fusA gene, recA gene, cox1 gene, nif13 gene, or a combination thereof.
 12. The method of claim 7, wherein the RNA is labeled with a fluorescent molecule.
 13. The method of claim 7, wherein the RNA is labeled with a non-fluorescent molecule.
 14. The method of claim 1, wherein in step f) the hybridized labeled RNA is imaged with a fluorescence scanner and fluorescence intensity is measured for each probe feature.
 15. The method of claim 1, wherein in step f) the detection of hybridization signal strength provides a determination of genes present in the community of organisms.
 16. The method of claim 1, wherein in step f) the detection of hybridization signal strength is used for normalization of the isotope signals detected in step i).
 17. The method of claim 1, wherein in step i) the hybridized unlabeled fragmented RNA are imaged with a secondary ion mass spectrometer and isotope ratios are measured for each probe feature.
 18. The method of claim 1, wherein in step i) the hybridized unlabeled fragmented RNA are imaged with a nano-scale secondary ion mass spectrometer device and isotope ratios are measured for each probe feature.
 19. The method of claim 1, wherein in step e) the substrate is a solid planar substrate, a microarray slide, spheres or beads, wherein the substrate is comprised of silicon, glass, metals, semiconductor materials, polymers or plastics.
 20. The method of claim 18, wherein in step h) the responsive probe substrate is a solid planar substrate, a microarray slide, spheres or beads, wherein the responsive probe substrate is comprised of silicon, glass, metals, semiconductor materials, polymers or plastics.
 21. The method of claim 20, wherein the responsive probe substrate is a solid planar substrate coated with indium tin oxide (ITO).
 22. The method of claim 1, wherein in step e) the set of oligonucleotide probes is identified and selected from unique sequence regions of the fragmented RNA.
 23. A method for determination of stable isotope incorporation in a community of organisms comprising the steps of: a) supplying said community of organisms with at least two substrates simultaneously for a defined period of time, wherein each of the at least two substrates is labeled with a different stable isotope; b) extracting RNA from the organisms; c) fragmenting said RNA to provide fragmented RNA, generating cDNAs from a fraction of said fragmented RNA and sequencing said cDNAs; d) designing a first set of oligonucleotide probes based on unique sequence regions of said sequenced cDNAs generated from the fragmented RNA; e) labeling a fraction of the fragmented RNA with a detectable label to provide labeled fragmented RNA; f) hybridizing the labeled fragmented RNA to the first set of oligonucleotide probes, wherein the first set of oligonucleotide probes is an array of oligonucleotide probes attached to a substrate, and detecting hybridization signal strength of the labeled fragmented RNA hybridized to the first set of oligonucleotide probes to determine the community organism composition; g) identifying a responsive set of oligonucleotide probes based on the hybridization signal strength in step f); h) hybridizing a fraction of unlabeled fragmented RNA to a second array of oligonucleotide probes, wherein the second array comprises the responsive set of oligonucleotide probes attached to a conductive substrate; i) detecting the unlabeled fragmented RNA hybridized to the responsive set of probes to determine the stable isotope incorporation into the community of organisms using imaging mass spectrometry or spectroscopy.
 24. The method of claim 23, wherein said organism is a bacterium, archaea, virus, fungus, plant, arthropod, nematode, or other eukaryote.
 25. The method of claim 24, wherein said organism is a bacterium.
 26. The method of claim 23, wherein the stable isotopes are selected from the group consisting of ³H, ¹³C, ¹⁵N, and ¹⁸O.
 27. The method of claim 23, wherein in step b) the extracting step is carried out by physical or chemical cell lysis followed by affinity column purification.
 28. The method of claim 23, wherein in step c) the fragmenting step is carried out by using enzymes, chemicals, or heat, or a combination of these.
 29. The method of claim 23, wherein in step e) the RNA is labeled with a fluorescent molecule or a non-fluorescent molecule.
 30. The method of claim 23, wherein steps c) and e) are carried out concurrently.
 31. The method of claim 23, wherein step f) further comprises the steps of adding said labeled fragmented RNA to a hybridization solution and contacting said hybridization solution with the array of oligonucleotide probes.
 32. The method of claim 23, wherein the set of oligonucleotide probes comprises 16S rRNA phylogenetic oligonucleotide probes.
 33. The method of claim 32, wherein said set of 16S rRNA phylogenetic probes further comprises probes from the 16S rRNA gene, 23S rRNA gene, 5S rRNA gene, 5.8S rRNA gene, 12S rRNA gene, 18S rRNA gene, 28S rRNA gene, gyrB gene, rpoB gene, fusA gene, recA gene, cox1 gene, nif13 gene, or a combination thereof.
 34. The method of claim 29, wherein the RNA is labeled with a fluorescent molecule.
 35. The method of claim 29, wherein the RNA is labeled with a non-fluorescent molecule.
 36. The method of claim 23, wherein in step f) the hybridized labeled RNA is imaged with a fluorescence scanner and fluorescence intensity is measured for each probe feature.
 37. The method of claim 23, wherein in step f) the detection of hybridization signal strength provides a determination of genes present in the community of organisms.
 38. The method of claim 23, wherein in step f) the detection of hybridization signal strength is used for normalization of isotope signals detected in step i).
 39. The method of claim 23, wherein in step i) the hybridized unlabeled fragmented RNA are imaged with a secondary ion mass spectrometer and isotope ratios are measured for each probe feature.
 40. The method of claim 23, wherein in step i) the hybridized unlabeled fragmented RNA are imaged with a nano-scale secondary ion mass spectrometer device and isotope ratios are measured for each probe feature.
 41. The method of claim 23, wherein in step f) the substrate is a solid planar substrate, a microarray slide, spheres or beads, wherein the substrate is comprised of silicon, glass, metals, semiconductor materials, polymers or plastics.
 42. The method of claim 23, wherein in step h) the responsive probe substrate is a solid planar substrate, a microarray slide, spheres or beads, wherein the responsive probe substrate is comprised of silicon, glass, metals, semiconductor materials, polymers or plastics.
 43. The method of claim 25, wherein the responsive probe substrate is a solid planar substrate coated with indium tin oxide (ITO). 